Abstract:
OBJECTIVE:The aim of this study was to explore the effects of micro ribonucleic acid (miR)-506 and phosphatidylinositol 3-hydroxy kinase (PI3K)/protein kinase B (AKT) pathway on myocardial ischemia-reperfusion injury (MIRI) in rats. MATERIALS AND METHODS:A total of 90 healthy rats weighing 260-300 g were selected as research subjects, and divided into three groups, including: Control group (n=30), IR group (n=30), and miRNA treatment group (IR + miR-506 group, n=30). The model was successfully established via threading the coronary artery. The structural differences in myocardial tissues were observed via hematoxylin-eosin (HE) staining in each group. The mRNA expressions of miR-506 and PI3K in myocardial tissues were detected using fluorescence quantitative Polymerase Chain Reaction (qPCR). Meanwhile, AKT protein phosphorylation activity in myocardial tissues was detected as well. The apoptosis of myocardial tissues was detected via terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) staining. In addition, superoxide dismutase (SOD) activity and malondialdehyde (MDA) content in myocardial tissues were compared in each group. RESULTS:In Control group, no structural abnormalities were found in myocardial tissues, and no inflammatory cells were observed. In IR group, myocardial tissues were arranged disorderly, and inflammatory cell infiltration was found. In IR + miR-506 group, myocardial tissue lesions were milder than those in the IR group. qPCR results indicated that the mRNA expressions of miR-506 and PI3K in myocardial tissues were statistically different among groups (p<0.05), with the lowest in the IR group. The expression of miR-506 was evidently higher in IR + miR-506 group than that in the Control group (p<0.05). However, the mRNA expression of PI3K was significantly higher in the Control group than IR + miR-506 group (p<0.05). There was a significant positive correlation between the expressions of miR-506 and PI3K in each group (p<0.05). The phosphorylation activity of AKT protein in IR + miR-506 group was markedly higher than the other two groups (p<0.05). In addition, TUNEL staining demonstrated that the apoptosis rate in Control group, IR group and IR + miR-506 group was only 1.3%, 20.3%, and 9.8%, respectively. SOD activity was remarkably stronger in the Control group (62.7 U/mg pro) than the other two groups (p<0.05). In addition, MDA content was remarkably higher in IR group (0.747 nmol/mg pro) than that in the other two groups (p<0.05). CONCLUSIONS:MiR-506 is associated with myocardial injury in rats, which can alleviate myocardial injury through the PI3K/AKT signaling pathway.
journal_name
Eur Rev Med Pharmacol Scijournal_title
European review for medical and pharmacological sciencesauthors
Zhang M,Wang JY,Li L,Li GMdoi
10.26355/eurrev_202012_24193subject
Has Abstractpub_date
2020-12-01 00:00:00pages
12896-12903issue
24eissn
1128-3602issn
2284-0729journal_volume
24pub_type
杂志文章abstract:OBJECTIVE:Lung cancer is the leading cause of cancer-related mortality. Over 80% of all lung cancer cases are non-small-cell lung cancer (NSCLC) and approximately 5% of NSCLC patients are positive for anaplastic lymphoma kinase (ALK) gene rearrangement or fusion with echinoderm microtubule-associated protein-like 4 (EM...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章,meta分析,评审
doi:
更新日期:2017-08-01 00:00:00
abstract::Fluoxetine drops and marketed fluoxetine capsules had quite the same Cmax (50.25 +/- 4.43 vs 47.55 +/- 5.29 ng/ml), but significantly different AUC0-t (717.27 +/- 71.29 vs 644.91 +/- 78.91 ng/ml/h). Furthermore the drops were characterised by a very early Tmax (4.61 +/- 0.85 hours) with a highly significant difference...
journal_title:European review for medical and pharmacological sciences
pub_type: 临床试验,杂志文章
doi:
更新日期:2000-01-01 00:00:00
abstract::Pathogenesis of cyclic vomiting syndrome (CVS) is still uncertain. Aim of our study has been to verify the role of gastric emptying time in children affected by CVS. We studied 9 children with CVS who underwent measurement of gastric emptying time by real-time ultrasonography after administration of a mixed, solid-liq...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2004-11-01 00:00:00
abstract:OBJECTIVE:Lung cancer is one of the most common malignancies worldwide, the morbidity and mortality of which have been on rising in recent years. Moreover, lncRNAs have been implicated in the development of various cancers, as well as cancer treatment and prognosis. In this study, long non-coding RNA (lncRNA) MEG3, an ...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_201802_14384
更新日期:2018-02-01 00:00:00
abstract:OBJECTIVE:Osteoarthritis (OA) is one of the most common chronic joint diseases, caused by lesions in articular cartilage and synovial membranes. Synovitis is a major characteristic of OA, due to the proliferation of synoviocytes. Long noncoding RNAs (lncRNAs) have implicated to play an important role in many different ...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_201808_15705
更新日期:2018-08-01 00:00:00
abstract:OBJECTIVE:Emerging evidence has suggested that dysregulation of miR-874-3p may be involved in tumor development and progression in various types of cancers. However, its expression and biological function in esophageal squamous cell carcinoma (ESCC) remains unclear. The aim of this study was to explore the roles of miR...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_201811_16261
更新日期:2018-11-01 00:00:00
abstract::In 2015 bladder cancer was the fourth most frequent malignancy and the eighth cause of death for cancer. At diagnosis, about 30% of bladder cancer (BC) patients present a muscle-invasive bladder cancer (MIBC) and 5% a metastatic bladder carcinoma (MBC). For fit MBC patients, combination chemotherapy (CC) is the standa...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_202011_23795
更新日期:2020-11-01 00:00:00
abstract::Forty-year-old man was admitted to emergency department with complains of chest pain and dispnea after exposure the pepper gas that sprayed to environment during a social event. Physical examination and electrocardiogram was revealed acute myocardial infarction. ...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2012-03-01 00:00:00
abstract:OBJECTIVE:To investigate the correlations of inflammatory factors, interleukin-6 (IL-6), IL-10, IL-13 and tumor necrosis factor-α (TNF-α), and composition of bacterial flora in the peritoneal fluid with infertility in endometriosis patients. PATIENTS AND METHODS:A total of 55 patients diagnosed with endometriosis and ...
journal_title:European review for medical and pharmacological sciences
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doi:10.26355/eurrev_201805_14899
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abstract:OBJECTIVE:Patients with severe burns often develop acute lung injury (ALI), systemic inflammatory response syndrome (SIRS) often complicates with ALI. Sivelestat sodium hydrate is an effective drug against ALI. However, the mechanisms of this beneficial effect are still poorly understood. In the current study, we evalu...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2016-01-01 00:00:00
abstract:OBJECTIVE:Aberrant activation of (Wingless and mouse homolog Int-1) Wnt/β-catenin signaling pathways closely involved in the occurrence and progression of several types of human malignancies. This research was undertaken to elucidate the important role of (Wingless and mouse homolog Int-1) in lung cancer. PATIENTS AND...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_201803_14474
更新日期:2018-03-01 00:00:00
abstract:OBJECTIVE:The awareness of audio-vestibular side effects of drugs, such as hearing loss, tinnitus, dizziness and vertigo, has widely increased in the recent years. The present guide represents an update of the previous documents published by the authors in 2005 and 2011 on drug-induced ototoxicity and vestibulotoxicity...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_202008_22477
更新日期:2020-08-01 00:00:00
abstract:BACKGROUND:Coronary artery bypass grafting (CABG) is a revascularization technique that has been reported as having satisfactory survival rates in Hemodialysis (HD) dependent patients. AIM:The aim of this study was to determine morbidity and mortality of isolated CABG in HD-dependent patients and compare with non- ren...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2013-10-01 00:00:00
abstract::This article describes the management of acute asthma exacerbation in the Emergency Department (ED). An asthma exacerbation can be defined as clinical worsening of disease or an asymptomatic decrease in peak flows. Acute exacerbations of asthma may represent reactions to airway irritants or failures of chronic treatme...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章,评审
doi:
更新日期:2010-03-01 00:00:00
abstract:OBJECTIVE:This study aims to explore whether ZEB2-AS1 can promote the development of osteosarcoma by affecting the proliferation, invasion, and apoptosis of osteosarcoma cells. PATIENTS AND METHODS:Quantitative Real Time-Polymerase Chain Reaction (qRT-PCR) was performed to detect the ZEB2-AS1 expression in osteosarcom...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_202006_21637
更新日期:2020-06-01 00:00:00
abstract::Hepatic fibrosis and cirrhosis represent the main consequence of chronic liver diseases of different origin. Therefore, the clinical assessment of disease severity is a must in the management of patients with chronic liver damage. Although liver biopsy may be associated with sampling error, interobserver variability a...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章,评审
doi:
更新日期:2004-01-01 00:00:00
abstract:OBJECTIVE:The aim of this study was to investigate the influences of propofol on myocardial ischemia/reperfusion injury in rats through the Janus kinase/signal transducers and the activators of transcription (JAK/STAT) signaling pathway. MATERIALS AND METHODS:A total of 48 Sprague-Dawley (SD) rats were randomly divide...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_201907_18456
更新日期:2019-07-01 00:00:00
abstract:OBJECTIVE:The use of gonadotropin-releasing hormone agonist for ovulation triggering has become an intriguing topic in the last few years. As long as adequate luteal phase support is provided, it may be a valuable alternative to standard hCG triggering, associated with a significant reduction in OHSS incidence. Several...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2017-02-01 00:00:00
abstract::Sarcoidosis is a granulomatous disease of unknown origin, with pulmonary findings in more than 90% of patients. Extrapulmonary involvement is common and all organs can be involved (especially lymph nodes, eyes, joints, central nervous system) but it is rare to find an isolated extrapulmonary disease (less than 10% of ...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章,评审
doi:
更新日期:2009-03-01 00:00:00
abstract::Kaposi's sarcoma (KS) is a multicentric angioproliferative cancer of endothelial origin typically occurring in the context of immunodeficiency, i.e. coinfection with Human Immonodeficiency Virus (HIV) or transplantation. The incidence of KS has dramatically decreased in both US and Europe in the Highly Active Antiretr...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章,评审
doi:
更新日期:2013-09-01 00:00:00
abstract:OBJECTIVE:The aim of this study was to investigate the expression of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) in juvenile rats with nephrotic syndrome, and to explore its effects on inflammatory changes and renal injury. MATERIALS AND METHODS:24 Sprague-Dawley (SD) rats were randomly divi...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_201905_17831
更新日期:2019-05-01 00:00:00
abstract:OBJECTIVE:Ulcerative colitis (UC) is a kind of chronic inflammatory bowel diseases that seriously endangers human health. The pathogenesis of UC is closely related to the intestinal immune response. Cytokines exert a key role in the regulation of intestinal inflammatory and immune responses. Abnormalities in the functi...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_201808_15633
更新日期:2018-08-01 00:00:00
abstract:OBJECTIVE:Coronary artery ectasia (CAE) is defined as abnormal dilatation of coronary arteries. Inflammation is thought to be important in the pathogenesis of CAE. Red blood cell distribution width (RDW) is also an inflammatory marker. In this study, we examined the association between RDW levels and CAE severity. PAT...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2016-04-01 00:00:00
abstract:OBJECTIVE:The purpose of this study was to explore the influences of micro ribonucleic acid (miR)-708 on cerebral ischemia-reperfusion injury by regulating a disintegrin and metalloprotease 17 (ADAM17) in a targeted manner. MATERIALS AND METHODS:The rat model of middle cerebral artery occlusion (MCAO) was established,...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_202011_23835
更新日期:2020-11-01 00:00:00
abstract:OBJECTIVE:MicroRNA-610 (miR-610) functions as a tumor suppressor in various types of cancers. However, whether miR-610 acted as a functional miRNA in oral squamous cell carcinoma (OSCC) is still largely unknown. The current study was designed to explore the expression pattern and function of miR-610 in OSCC and to inve...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_201901_16764
更新日期:2019-01-01 00:00:00
abstract::Allergic bronchopulmonary aspergillosis (ABPA) occurs in 7-9% of patients with cystic fibrosis (CF) and causes a worsening of lung function and respiratory symptoms. Standard treatment of ABPA consists of oral steroids; however, higher corticosteroid therapy associated to antifungal agent (itraconazole) long-term trea...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2014-07-01 00:00:00
abstract:OBJECTIVE:Our goal was to evaluate the long-term clinical outcome of ankle arthrodesis, obtained by an extramedullary internal fixation with or without bone-grafting in the treatment of ankle' septic arthritis. PATIENTS AND METHODS:All patients treated with arthrodesis by extramedullary internal fixation for septic ar...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_201904_17483
更新日期:2019-04-01 00:00:00
abstract:BACKGROUND:Circadian rhythms tend to change as animals age; however, the molecular mechanisms underlying these are not yet fully understood. OBJECTIVE:To investigate whether the DNA methylation of clock genes changes with age and contributes to circadian dysfunction in aged animals. METHODS:We examined the methylatio...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2013-07-01 00:00:00
abstract:OBJECTIVE:The aim of this study was to investigate the effect of microRNA-214-5p (miR-214-5p) on spinal cord injury (SCI) and to explore the mechanism of action in pathophysiological relevance. MATERIALS AND METHODS:The model of SCI was successfully established in rats aged 6-8 weeks. The levels of the locomotor funct...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_201911_19536
更新日期:2019-11-01 00:00:00
abstract:OBJECTIVE:Coronary artery disease (CAD) is a life-threatening disease and is caused by various factors, with genetic variation being an important risk factor. The association between X-ray repair cross-complementing group 1 (XRCC1) polymorphisms and CAD has been extensively studied with conflicting results. We performe...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章,meta分析
doi:
更新日期:2017-04-01 00:00:00