Abstract:
OBJECTIVE:To explore possible mechanism of ERBB2 gene expression silencing mediating mitogen-activated protein kinase 1/mitogen-activated protein kinase 3 (MAPK1/MAPK3) signaling pathway on proliferation, migration, and invasion of ovarian cancer cells. PATIENTS AND METHODS:A total of 240 cancer specimens were collected in patients with epithelial ovarian cancer intraoperatively in our hospital from January 2015 to January 2018. Expressions of ERBB2, MAPK1, and MAPK3 in tissues were detected by immunohistochemistry. Following the culture of ovarian cancer cell lines, target cell line with high expression of ERBB2 was screened by qRT-PCR. Cell grouping was performed with four groups after transfection, including Blank group, negative control (NC) group, ERBB2 shRNA group, and ERBB2 overexpression group (shorted as ERBB2 group). The expression levels of ERBB2, MAPK1, MAPK3, vascular endothelial growth factor (VEGF), metalloproteases-2 (MMP-2), and tissue inhibitor of metalloproteases-2 (TIMP-2) were detected by qRT-PCR in different transfection groups, followed by the detection of protein expressions with Western blot. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was used to test the proliferation activity of each group after transfection, while transwell assay and scratch test explored cell invasion and migration in each group, respectively. RESULTS:Immunohistochemistry showed that the positive rates of ERBB2, MAPK1, and MAPK3 in ovarian cancer tissues were significantly increased than those in adjacent normal epithelial tissues. In the cell experiment, ERBB2 gene was highly expressed in SKOV3 ovarian cancer cell line. There was no significant difference in each index between Blank group and NC group (p > 0.05). Compared with Blank group and NC group, the expression levels of ERBB2, MAPK1, MAPK3, VEGF, and MMP-2 in ERBB2 shRNA group decreased significantly, TIMP-2 increased markedly, and proliferation, invasion, and migration abilities of cells decreased markedly after transfection, showing statistically significant differences (All p < 0.05). By contrast, the expression levels of ERBB2, MAPK1, MAPK3, VEGF, and MMP-2 increased remarkably in ERBB2 group, while TIMP-2 decreased significantly, and cell proliferation, invasion, and migration ability increased evidently after transfection, with statistically significant differences (All p < 0.05). CONCLUSIONS:Silencing ERBB2 gene expression may inhibit the activation of MAPK1/MAPK3 signaling pathway and thus suppress the proliferation, invasion, and migration of ovarian cancer cells. Overexpression of ERBB2 gene can reverse those trends, which in turn support the role of ERBB2 gene expression silencing in molecular targeted therapy of ovarian cancer.
journal_name
Eur Rev Med Pharmacol Scijournal_title
European review for medical and pharmacological sciencesauthors
Yu TT,Wang CY,Tong Rdoi
10.26355/eurrev_202005_21309subject
Has Abstractpub_date
2020-05-01 00:00:00pages
5267-5280issue
10eissn
1128-3602issn
2284-0729journal_volume
24pub_type
杂志文章abstract:OBJECTIVE:The aim of this study was to investigate the effect of long non-coding ribonucleic acid (lncRNA) growth arrest specific 5 (GAS5) on acute myocardial infarction (AMI) model rats and to explore its regulatory mechanism. MATERIALS AND METHODS:The rat model of AMI was established by subcutaneous injection of iso...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_201910_19173
更新日期:2019-10-01 00:00:00
abstract:OBJECTIVE:Alcoholic hepatitis (AH) is a type of alcoholic liver disorder caused by overconsumption of alcohol. The involvement of several transcription factors (TF), as the main regulators of disease related gene expression has been documented previously. However, despite the importance of analysis of gene regulatory n...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2015-06-01 00:00:00
abstract:AIM:The efficacy of dietary inclusion of various parts of mangosteen (Garcinia mangostana L.) extract on growth and hematological parameters of African catfish (Clarias gariepinus) fingerlings were investigated. MATERIAL AND METHODS:Ninety six fingerlings were randomly distributed into each of ten 20L plastic aquaria....
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2010-07-01 00:00:00
abstract:OBJECTIVE:Long non-coding RNA (lncRNA) LINC00467 was found to be upregulated in glioma tissues by analyzing The Cancer Genome Atlas (TCGA) database. This study aims to uncover the biological role of LINC00467 in influencing the progression of glioma and to provide novel directions for clinical treatment of glioma. PAT...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_202001_20057
更新日期:2020-01-01 00:00:00
abstract:OBJECTIVE:Perlecan, which is also called heparan sulfate proteoglycan 2 (HSPG2), is a protein encoded by the HSPG2 gene that maps to 1p36.12 in the human genome. In this study, we assessed the independent prognostic value of HSPG2 in terms of overall survival (OS) and recurrence-free survival (RFS) in patients with LGG...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_201810_16154
更新日期:2018-10-01 00:00:00
abstract:OBJECTIVES:The purpose of the study was to investigate the clinical implantation protocol of custom-made artificial semi-knee joint based on computer-aided design so as to improve the limb salvage efficiency. MATERIALS AND METHODS:The custom-made artificial semi-knee joint was designed and manufactured based on rapid ...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2014-01-01 00:00:00
abstract:OBJECTIVE:To explore the correlation between angiotensin II type 1 receptor (AT1R) gene polymorphism and epilepsy secondary to cerebral infarction and its significance for the diagnosis of this disease. PATIENTS AND METHODS:A total of 200 patients with epilepsy secondary to cerebral infarction were enrolled from our h...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_202006_21677
更新日期:2020-06-01 00:00:00
abstract:OBJECTIVE:On December 8, 2019, many cases of pneumonia with unknown etiology were first reported in Wuhan, China, subsequently identified as a novel coronavirus infection aroused worldwide concern. As the outbreak is ongoing, more and more researchers focused interest on the COVID-19. Therefore, we retrospectively anal...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_202003_20712
更新日期:2020-03-01 00:00:00
abstract:OBJECTIVE:This study aims to clarify the influences of CHCHD2 and MMP2 on docetaxel resistance in breast cancer. PATIENTS AND METHODS:Differential levels of CHCHD2 in breast cancer and para-tumor tissues were detected. The relationship between CHCHD2 and prognosis in breast cancer patients was analyzed. After generati...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_202006_21541
更新日期:2020-06-01 00:00:00
abstract:OBJECTIVE:Atherosclerosis is featured as artery wall thickness as a result of invasion and accumulation of white blood cells and proliferation of intimal smooth muscle cells. Endothelial dysfunction has been linked to a variety of vascular diseases, including atherosclerosis. MicroRNAs play essential roles during the a...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2016-07-01 00:00:00
abstract:BACKGROUND/AIMS:The risk of wound contamination in high risk of infection patients after abdominal operations is well recognised. Preincisional intraparietal injection of antibiotics is used for the prophylaxis of postoperative surgical infections. Whether topically injected antibiotics remain primarily in the surgical...
journal_title:European review for medical and pharmacological sciences
pub_type: 临床试验,杂志文章
doi:
更新日期:1998-05-01 00:00:00
abstract:OBJECTIVE:To study the effects of chronic virus-mediated micro ribonucleic acid miR-27a on proliferation and migration capacities of liver cancer cells. PATIENTS AND METHODS:A total of 60 patients with primary liver cancer from January 2015 to December 2016 were selected as observation group, 60 patients with chronic ...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_201809_15809
更新日期:2018-09-01 00:00:00
abstract::Antifungal agents have greatly contributed to the improvement of public health. Nevertheless, antifungal resistant pathogens have increased during the past decade, becoming a serious concern. Candida albicans has been the most extensively studied pathogen in antifungal resistance because of their morbidity and mortali...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章,评审
doi:
更新日期:2004-03-01 00:00:00
abstract:OBJECTIVE:To investigate whether SENP3 protects H9C2 cells from apoptosis triggered by H/R through the signal transducer and activator of transcription 3 (STAT3) pathway. MATERIALS AND METHODS:Male C57BL mice were cultured and mouse models of myocardial I/RI were established. At the same time, cardiomyoblast H9C2 cell...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_201805_14975
更新日期:2018-05-01 00:00:00
abstract:OBJECTIVE:Colorectal cancer (CRC) is a common human malignancy and is the second leading cause of cancer deaths worldwide with a dismal prognosis. Previous investigations have shown that miR-340 can modulate the metabolism of CRC cells. The aim of this report is to study the role of miR-340 in the development and progr...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2017-06-01 00:00:00
abstract:UNLABELLED:BACKGROUND, OBJECTIVES: Pancreatic cancer ranks fourth for cancer mortality for men and women in the United States. This is a particularly devastating cancer since the case-fatality proportion approaches 90% within 12 months following diagnosis. Therefore, understanding the etiology and identifying the risk ...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章,评审
doi:
更新日期:2012-02-01 00:00:00
abstract:OBJECTIVE:The aim of this study was to investigate the expression characteristics of LINC01857 in gastric cancer (GCa), and to further study whether it could promote GCa development by modulating microRNA-200b. PATIENTS AND METHODS:Quantitative Real Time-Polymerase Chain Reaction (qRT-PCR) was used to examine LINC0185...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_202004_20827
更新日期:2020-04-01 00:00:00
abstract:OBJECTIVE:The aim of this qualitative review is to provide an update on the current understanding of the genetic determinants of lipedema and to develop a genetic test to differentiate lipedema from other diagnoses. MATERIALS AND METHODS:An electronic search was conducted in MEDLINE, PubMed, and Scopus for articles pu...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章,评审
doi:10.26355/eurrev_201907_18292
更新日期:2019-07-01 00:00:00
abstract:OBJECTIVE:To explore the clinical efficacy of aneurysm embolization and cerebrospinal fluid (CSF) replacement in the treatment of aneurysmal subarachnoid hemorrhage (SAH). PATIENTS AND METHODS:Seventy-nine patients with grade III-IV aneurysmal subarachnoid hemorrhage, who were treated in the hospital from Jan. 2012 to...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2015-01-01 00:00:00
abstract::Mucoepidermoid carcinoma (MEC) of the skin is an extremely rare neoplasm but is common in the major and minor salivary glands accounting of approximately 30% of all malignant tumors arising from these glands. Cutaneous involvement should be carefully assessed to exclude the possibility of metastases from distant sites...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2012-10-01 00:00:00
abstract:OBJECTIVE:Cervical cancer is a common malignant tumor in women with increased incidence and younger onset age. As a curable tumor, timely diagnosis and early intervention are critical. Based on the golden standard of cervical tissues pathology examination, we investigated the value of free body of reduced iron protopor...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2017-05-01 00:00:00
abstract:OBJECTIVE:To study the protective effect of glutamine on the intestinal tissues of septic rats by regulating the nuclear factor-κB (NF-κB) pathway. MATERIALS AND METHODS:A total of 30 rats were divided into the Sham group, Model group, and Glutamine group using a random number table. The changes in the intestinal tiss...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_201908_18646
更新日期:2019-08-01 00:00:00
abstract:OBJECTIVE:To study the safety and efficacy of endoscopic mucosal resection (EMR) combined with chemotherapy in patients with early gastric cancer. PATIENTS AND METHODS:Continuously, 112 patients of early gastric cancer (IA-IB) were chosen to receive the treatment of EMR. The 112 patients were divided into two groups, ...
journal_title:European review for medical and pharmacological sciences
pub_type: 临床试验,杂志文章
doi:
更新日期:2016-06-01 00:00:00
abstract:OBJECTIVE:The incidence and death rate of lung cancer has been rising year by year. Non-small cell lung cancer (NSCLC) seriously affects people's health and quality of life. This study was designed to explore the functional role of long-chain non-coding RNA (LncRNA) MIR503HG in the development of NSCLC. PATIENTS AND M...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_201912_19785
更新日期:2019-12-01 00:00:00
abstract:OBJECTIVE:Melatonin (MT) is a hormone mainly produced by the pineal gland. It may be involved in the regulation of nociception, the mechanisms of which remain unclear. In the present study, electrophysiological effects of MT on neurons were studied. MATERIALS AND METHODS:The cultured neurons were isolated from Sprague...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_201805_15086
更新日期:2018-05-01 00:00:00
abstract:OBJECTIVE:The X-linked inhibitor of apoptosis protein (XIAP) is associated with the development of various tumors. The abnormal miR-142 expression is associated with the onset of lung cancer. Bioinformatics analysis revealed a targeted relationship between miR-142 and XIAP. This report investigated whether miR-142 play...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_201909_18852
更新日期:2019-09-01 00:00:00
abstract:OBJECTIVE:After MI pathological LV remodeling is one of the major causes of death. We previously showed the NO mediated beneficial effects of nebivolol in rat MI model, in this study we aimed to evaluate the NOS related mechanisms in this phenomenon. MATERIALS AND METHODS:Rats were divided into four groups: sham opera...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2015-12-01 00:00:00
abstract:OBJECTIVE:Over-proliferation of mesangial cells is the major pathological change of mesangial proliferative glomerulonephritis (MPGN). PTEN-PI3K/AKT pathway plays a role in regulating proliferation of mesangial cells. Anti-thymocyte serum nephritis (ATSN) is a widely used animal model for studying MPGN. This study esta...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2017-08-01 00:00:00
abstract:BACKGROUND:Bisphosphonate-Related Osteonecrosis of the Jaw (BRONJ) are the result of the assumption of such drugs. The most widely used molecules are pamidronate and zoledronic acid, which are pyrophosphate analogues and are usually given to patient with bone remodelling diseases. International literature reports showe...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2012-11-01 00:00:00
abstract:AIM:We aimed to determine the relationship between blood lactate, carboxy-hemoglobin (COHb) levels and the severity of clinical findings in patients with CO poisoning. PATIENTS AND METHODS:Patients over 18 years old and of both gender who were admitted to Emergency Department with the diagnosis of CO poisoning between...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章,随机对照试验
doi:
更新日期:2014-01-01 00:00:00