Abstract:
OBJECTIVE:Over-proliferation of mesangial cells is the major pathological change of mesangial proliferative glomerulonephritis (MPGN). PTEN-PI3K/AKT pathway plays a role in regulating proliferation of mesangial cells. Anti-thymocyte serum nephritis (ATSN) is a widely used animal model for studying MPGN. This study established ATSN model, on which the role of PTEN-PI3K/AKT signal pathway in MPGN pathogenesis was investigated. MATERIALS AND METHODS:ASTN rat model was established in parallel with control group. Protein expressions of PTEN, p-AKT, PCNA, Cyclin D1 and Bcl-2 were quantified, along with glomerular mesangial cell (GMC) counting. Rat mesangial cell (RMC) was treated with 0 or 10 ng/mL IL-6, followed by flow cytometry analysis for apoptosis, cycle and PCNA expression. Expressions of PTEN, p-AKT, PCNA, Cyclin D1 and Bcl-2 were measured. RMC was treated with pSicoR-PTEN and/or LY294002, followed by the treatment of 10 ng/mL IL-6 for 48 h. Cell apoptosis, cycle, PCNA expression and protein expression were measured. RESULTS:Lower PTEN expression was found in renal cortex of ATSN rats, along with increasing levels of p-AKT, PCNA, Cyclin D1, Bcl-2, and higher GMCs, compared to that in control rats. IL-6 treatment increased protein expression in RMC, facilitated cell proliferation and cycle progression and suppressed apoptosis. Over-expression of PTEN and/or LY294002 remarkably decreased protein expression in RMC, inhibited the effect of IL-6 on proliferation, and induced cell apoptosis and cycle arrest. CONCLUSIONS:The down-regulation of PTEN played a role in enhancing PI3K/AKT pathway activity, facilitating GMC proliferation and MPGN pathogenesis.
journal_name
Eur Rev Med Pharmacol Scijournal_title
European review for medical and pharmacological sciencesauthors
Gao HY,Han CXsubject
Has Abstractpub_date
2017-08-01 00:00:00pages
3634-3641issue
16eissn
1128-3602issn
2284-0729journal_volume
21pub_type
杂志文章abstract:OBJECTIVE:The aim of this study was to investigate the relationship between micro ribonucleic acid (miR)-152-3p and chronic myeloid leukemia (CML), and to explore the underlying mechanism. PATIENTS AND METHODS:The expression level of miR-152-3p in the bone marrow of 40 CML patients and 30 normal controls was detected ...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_201812_16646
更新日期:2018-12-01 00:00:00
abstract:OBJECTIVE:DJ-1 is a negative regulator of PTEN and plays a role in tumorigenesis. Abnormal miR-203 expression is associated with pancreatic cancer. Bioinformatics analysis showed a targeted relationship between miR-203 and DJ-1 3'-UTR. This study investigated whether miR-203 regulates DJ-1 expression and its role in pa...
journal_title:European review for medical and pharmacological sciences
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journal_title:European review for medical and pharmacological sciences
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abstract:OBJECTIVE:This review paper aims to summarize the current state of knowledge on the role of the pneumologist in the diagnosis and respiratory treatment of children affected by obstructive Sleep Disordered Breathing (SDB). MATERIALS AND METHODS:A literature review has been performed on the following topics: obstructive...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章,评审
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abstract:OBJECTIVE:Lung cancer is one of the most common malignancies worldwide, the morbidity and mortality of which have been on rising in recent years. Moreover, lncRNAs have been implicated in the development of various cancers, as well as cancer treatment and prognosis. In this study, long non-coding RNA (lncRNA) MEG3, an ...
journal_title:European review for medical and pharmacological sciences
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doi:10.26355/eurrev_201802_14384
更新日期:2018-02-01 00:00:00
abstract::Baker's asthma, like other forms of occupational asthma is probably the most serious manifestation of occupational allergy among bakery workers. It is caused by immunologic sensitization and subsequent allergic reactions in the airways to occupational specific airborne allergens. Skin Prick Tests (SPTs) play an import...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章,评审
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doi:10.26355/eurrev_202011_23835
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journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2015-12-01 00:00:00
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journal_title:European review for medical and pharmacological sciences
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journal_title:European review for medical and pharmacological sciences
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journal_title:European review for medical and pharmacological sciences
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更新日期:2003-09-01 00:00:00
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journal_title:European review for medical and pharmacological sciences
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更新日期:2017-10-01 00:00:00
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journal_title:European review for medical and pharmacological sciences
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更新日期:2018-12-01 00:00:00
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journal_title:European review for medical and pharmacological sciences
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journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2017-05-01 00:00:00
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journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2012-10-01 00:00:00
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journal_title:European review for medical and pharmacological sciences
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pub_type: 杂志文章,随机对照试验
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更新日期:2017-10-01 00:00:00
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journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
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