Abstract:
OBJECTIVE:To study the effects of chronic virus-mediated micro ribonucleic acid miR-27a on proliferation and migration capacities of liver cancer cells. PATIENTS AND METHODS:A total of 60 patients with primary liver cancer from January 2015 to December 2016 were selected as observation group, 60 patients with chronic liver disease were selected as control group, and another 60 healthy subjects who received physical examination during the same period were selected as healthy group. All patients received serum miRNA detection. The different expressions of serum miRNAs in healthy people, patients with chronic liver disease, and patients with liver cancer were analyzed. The correlations of miR-27a with growth and proliferation of liver cancer MCC-7721 cells were studied. RESULTS:The levels of miR-27a and miR-664b in subjects in control group and healthy group were significantly lower than those in observation group, but the expression levels of miR-30a were statistically elevated (p<0.05). The expression of serum miR-27a in liver cancer patients with higher tumor-node-metastasis (TNM) staging (stage III-IV, number of tumors >1, tumor size >5 cm, and vascular invasion) was significantly higher than those in patients with lower TNM staging (stage I-II, number of tumors =1, tumor size ≤5 cm, and no vascular invasion) (p<0.05). At 48 h and 72 h after transfection, the proliferation of liver cancer MCC-7721 cells was significantly enhanced compared to that in non-transfection group (p<0.05). The level of miR-27a was significantly upregulated in cisplatin-resistant liver cancer A549/CDDP cells compared with that in parental A549 cells. MiR-27a regulated epithelial-mesenchymal transition (EMT) and cisplatin resistance in vitro, while modulated the in vivo response of liver cancer cells to cisplatin. Further studies identified the Raf kinase inhibitor protein (RKIP) as a direct and functional target of miR-27a. The knockdown of RKIP by RNAi showed a similar effect to ectopic miR-27a expression, whereas the over-expression of RKIP weakened the function of miR-27a in liver cancer cells. CONCLUSIONS:MiR-27a participated in the proliferation and migration capacities of liver cancer cells and influenced the effect of cisplatin via targeting RKIP. The high expression of miR-27a is closely related to the malignant degree of liver cancer, which provides guidance for the diagnosis, targeted therapy, and prognostic evaluation of liver cancer.
journal_name
Eur Rev Med Pharmacol Scijournal_title
European review for medical and pharmacological sciencesauthors
Li W,Yu ZX,Ma BFdoi
10.26355/eurrev_201809_15809subject
Has Abstractpub_date
2018-09-01 00:00:00pages
5490-5498issue
17eissn
1128-3602issn
2284-0729journal_volume
22pub_type
杂志文章abstract:OBJECTIVE:To evaluate the recurrent ischemic events and risk factors in patients with symptomatic intracranial artery stenosis. PATIENTS AND METHODS:Patients with acute cerebral infarction or transient ischemia attach (TIA) and intracranial arterial stenosis confirmed through CTA examination, were enrolled from the De...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2015-01-01 00:00:00
abstract::Surgery, chemotherapy and radiotherapy have been the main pillars of cancer treatment. Some of the recent improvements in survival of other cancers can be attributed to novel treatment therapies. Such therapies mostly target specific molecules involved in cancer progression and metastasis. Development and clinical int...
journal_title:European review for medical and pharmacological sciences
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abstract::Cryptorchidism is the most frequent defect of the male urogenital tract at birth. It represents a risk factor for primitive testiculopathy associated with long-term complications (infertility, testicular neoplasia, and hormonal changes). An only consensus exists: "children with bilateral cryptorchidism who are not tre...
journal_title:European review for medical and pharmacological sciences
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doi:
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abstract:BACKGROUND:Traumatic brain injury (TBI) is a serious neurodisorder commonly caused by sports related events or violence. It is the leading cause of disability in people under 40. AIM:In order to elucidate the molecular mechanism of the secondary injury after TBI. MATERIALS AND METHODS:In this study, we downloaded gen...
journal_title:European review for medical and pharmacological sciences
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doi:
更新日期:2014-01-01 00:00:00
abstract:OBJECTIVE:Increasing evidence indicates that dysregulation of miRNAs is involved in tumor progression and development. We aimed to determine potential values of miR-629 as a serum diagnostic and prognostic biomarker in pancreatic cancer (PC). PATIENTS AND METHODS:MiR-629 expression levels in PC tissues and serum were ...
journal_title:European review for medical and pharmacological sciences
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doi:10.26355/eurrev_201808_15715
更新日期:2018-08-01 00:00:00
abstract:OBJECTIVE:We investigated whether DHEA supplementation had an impact on ovarian reserve parameters and pregnancy rates in patients with poor ovarian response (POR) and primary ovarian insufficiency (POI). PATIENTS AND METHODS:A total of 34 people, 6 patients with POI and 28 patients with POR, were included in the stud...
journal_title:European review for medical and pharmacological sciences
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doi:10.26355/eurrev_202009_22856
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abstract:OBJECTIVE:The aim of this work was to explore whether lncRNA-MEG3 could serve as a serum biomarker for diagnosing chronic hepatitis B (CHB) and improve the early diagnostic and treatment efficacies. PATIENTS AND METHODS:Serum level of lncRNA-MEG3 in CHB patients and healthy controls was detected by quantitative Real T...
journal_title:European review for medical and pharmacological sciences
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doi:10.26355/eurrev_201905_17943
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abstract:OBJECTIVE:The aim of this study was to evaluate the influence of long non-coding ribonucleic acid (lncRNA) antisense non-coding RNA in the INK4 locus (ANRIL) on neuronal apoptosis in rats with cerebral infarction (CI), and to further explore the underlying mechanism of lncRNA ANRIL in the occurrence and development of ...
journal_title:European review for medical and pharmacological sciences
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doi:10.26355/eurrev_201911_19577
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journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章,评审
doi:
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journal_title:European review for medical and pharmacological sciences
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doi:10.26355/eurrev_201912_19774
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journal_title:European review for medical and pharmacological sciences
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doi:
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journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2010-06-01 00:00:00
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journal_title:European review for medical and pharmacological sciences
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journal_title:European review for medical and pharmacological sciences
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doi:
更新日期:2015-04-01 00:00:00
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journal_title:European review for medical and pharmacological sciences
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doi:10.26355/eurrev_201905_17821
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journal_title:European review for medical and pharmacological sciences
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journal_title:European review for medical and pharmacological sciences
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journal_title:European review for medical and pharmacological sciences
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doi:
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journal_title:European review for medical and pharmacological sciences
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journal_title:European review for medical and pharmacological sciences
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journal_title:European review for medical and pharmacological sciences
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journal_title:European review for medical and pharmacological sciences
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doi:
更新日期:2017-10-01 00:00:00
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journal_title:European review for medical and pharmacological sciences
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journal_title:European review for medical and pharmacological sciences
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