Abstract:
:This study demonstrates the removal of virus particles from B95-8 host cells that maintain normal activity under optimal osmotic pressure. After infecting B95-8 cells with Epstein-Barr virus (EBV) particles, the cells were treated with isosmotic solution [0.90% NaCl (330 mOsm/kg H(2)O)], hyposmotic solutions [0.36% NaCl (115 mOsm/kg H(2)O) and 0.27% NaCl (93 mOsm/kg H(2)O)] and distilled water. The pumping levels of virus particles were observed by inverse phase contrast microscopy and transmission electron microscopy (TEM). After treatment with the hyposmotic solutions, the following results were observed: firstly, after culturing for 24 and 48 h, the B95-8 cells in the hyposmotic solutions grew as well as the cells cultured in the isosmotic solution. Secondly, the virus particles in the B95-8 host cells overflowed onto the surface of the cells, while the organelle structures remained intact. This phenomenon was repeated in the removal of human immunodeficiency virus (HIV) from leukomonocytes. By optimizing the osmotic pressure, the activity of the B95-8 host cells was retained and the EBV particles were transported from the cells onto the cell surface.
journal_name
Exp Ther Medjournal_title
Experimental and therapeutic medicineauthors
Pan M,Shen J,Cai Jdoi
10.3892/etm.2013.907subject
Has Abstractpub_date
2013-03-01 00:00:00pages
718-722issue
3eissn
1792-0981issn
1792-1015pii
etm-05-03-0718journal_volume
5pub_type
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journal_title:Experimental and therapeutic medicine
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journal_title:Experimental and therapeutic medicine
pub_type: 杂志文章
doi:10.3892/etm.2014.1697
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abstract::The aim of this study was to investigate whether resolvin E1 (RvE1) protects against hepatic fibrosis in a murine model of liver fibrosis induced by Schistosoma japonicum infection. A total of 30 pathogen-free Kunming mice were randomly and equally divided into three groups: Control (uninfected, untreated), model (inf...
journal_title:Experimental and therapeutic medicine
pub_type: 杂志文章
doi:10.3892/etm.2014.1641
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journal_title:Experimental and therapeutic medicine
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abstract::Currently available antiepileptic drugs are effective; however, frequently associated with adverse effects that limit their therapeutic value. Compounds that target the molecular events underlying epilepsy, with minor or no adverse effects, would be of clinical value. Matrix metalloproteinase-9 (MMP-9) and the brain-d...
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doi:10.3892/etm.2019.7282
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abstract::Neuregulin-1 (NRG-1) is a cardioactive growth factor released from endothelial cells. However, the effect of NRG-1 on ventricular remodeling in diabetic cardiomyopathy (DCM) remains unclear. The aim of the present study was to investigate the pathophysiological role of NRG-1 in a rat model of DCM. Rat cardiac microvas...
journal_title:Experimental and therapeutic medicine
pub_type: 杂志文章
doi:10.3892/etm.2013.1273
更新日期:2013-11-01 00:00:00
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journal_title:Experimental and therapeutic medicine
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doi:10.3892/etm.2016.3119
更新日期:2016-05-01 00:00:00
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pub_type: 杂志文章
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abstract::[This corrects the article DOI: 10.3892/etm.2019.8114.]. ...
journal_title:Experimental and therapeutic medicine
pub_type: 已发布勘误
doi:10.3892/etm.2020.9581
更新日期:2021-02-01 00:00:00