Abstract:
:The aim of this study was to observe the effects of different types of fluid resuscitation on hepatic mitochondria and apoptosis in hemorrhagic shock, and the corresponding mechanisms. Forty rats were divided into five groups: Sham surgery (Sham group), shock (Shock group), Ringer's lactate resuscitation (RL group), hydroxyethyl starch resuscitation (HES group) and autologous blood resuscitation (BL group). A model of hemorrhagic shock was successfully induced in the latter four groups. The recovery objective was to maintain the mean arterial pressure (MAP) of the rats at 80 mmHg. Two hours after the end of the recovery experiment, fresh liver samples were examined in order to observe the changes in the morphology and mitochondrial membrane potential (ΔΨm). In addition, the levels of succinate dehydrogenase (SDH) activity were assessed, and a terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay was conducted to evaluate the level of apoptosis in the liver cells. In the Shock, RL, HES and BL groups, mitochondrial ultrastructural damage in the liver cells, significant reductions in liver cell function, liver ΔΨm and SDH activity, and the apoptosis of hepatocytes were more apparent compared with those in the Sham group. In the BL group, compared with the RL and HES groups, the injuries to the mitochondrial ultrastructure and liver cell function were significantly reduced, the hepatic ΔΨm and SDH activity were significantly increased and the hepatocyte apoptosis index (AI) was significantly reduced (P<0.05). In conclusion, in a rat model of hemorrhagic shock, different methods of fluid resuscitation may improve the liver cells with regard to mitochondrial ultrastructure and function, the stability of liver ΔΨm, the activity of SDH and the inhibition of liver cell apoptosis. The results indicate that infusion with autologous blood followed by RL solution is a preferable method of fluid resuscitation compared with HES.
journal_name
Exp Ther Medjournal_title
Experimental and therapeutic medicineauthors
Cheng Q,Yang G,Ma J,Li J,Shan Qdoi
10.3892/etm.2013.1447subject
Has Abstractpub_date
2014-02-01 00:00:00pages
335-342issue
2eissn
1792-0981issn
1792-1015pii
etm-07-02-0335journal_volume
7pub_type
杂志文章abstract::The radial force of esophageal stents may not completely change during extraction and therefore, the procedure of stent removal may cause tissue damage. The present study reports the manufacture of 2 novel detachable stents, which were designed to reduce tissue damage through their capacity to be taken or fall apart p...
journal_title:Experimental and therapeutic medicine
pub_type: 杂志文章
doi:10.3892/etm.2019.8190
更新日期:2020-01-01 00:00:00
abstract::Achondroplasia (ACH) is a hereditary dwarfism caused by the disturbed proliferation and differentiation of growth plate chondrocytes, followed by impaired endochondral bone growth. ACH is caused by mutations in the gene encoding the transmembrane receptor, fibroblast growth factor receptor 3 (FGFR3). In total, >90% of...
journal_title:Experimental and therapeutic medicine
pub_type: 杂志文章
doi:10.3892/etm.2015.2324
更新日期:2015-05-01 00:00:00
abstract::Lung cancer is among the most common causes of cancer-related mortality. It has a high mortality rate and resistance to chemotherapy due to its high metastatic potential. Naringenin, a bioactive compound identified in several fruits, displays anti-inflammatory and antitumor effects. Furthermore, naringenin mitigates t...
journal_title:Experimental and therapeutic medicine
pub_type: 杂志文章
doi:10.3892/etm.2016.3994
更新日期:2017-02-01 00:00:00
abstract::Esthesioneuroblastoma (ENB), also known as olfactory neuroblastoma, is a rare malignant tumor that accounts for 3% of all tumors of the nasal cavity. The incidence of ENB is 0.4 cases per million in the general population, and the most common symptoms are nasal obstruction and epistaxis. Previous studies have indicate...
journal_title:Experimental and therapeutic medicine
pub_type: 杂志文章
doi:10.3892/etm.2016.3732
更新日期:2016-11-01 00:00:00
abstract::The present study aimed to investigate the expression of complement component 1, q subcomponent-binding protein (C1QBP) in colon cancer cells, and identify proteins that interact with C1QBP. Total proteins were extracted from both the tumor and normal tissues of 22 patients with colon cancer and analyzed using liquid ...
journal_title:Experimental and therapeutic medicine
pub_type: 杂志文章
doi:10.3892/etm.2017.4249
更新日期:2017-05-01 00:00:00
abstract::Intactable epilepsy (IE) is relatively common in pediatric epilepsy. The resistance mechanism of IE has been previously investigated. Multidrug-resistant associated protein 1 (MRP1) and MRP2 are associated with drug transport. The aim of the present study was to investigate the expression of MRP1 and MRP2 in periphera...
journal_title:Experimental and therapeutic medicine
pub_type: 杂志文章
doi:10.3892/etm.2015.2746
更新日期:2015-11-01 00:00:00
abstract::Immunomodulatory or immunosuppressive properties of bone marrow-derived mesenchymal stem cells (BM-MSCs) facilitate the treatment of acute respiratory distress syndrome and acute lung injury (ALI). Dysregulated miRNA (miRNA or miR) expression associated with the effects of BM-MSCs was assessed in a rat model of lipopo...
journal_title:Experimental and therapeutic medicine
pub_type: 杂志文章
doi:10.3892/etm.2018.6118
更新日期:2018-06-01 00:00:00
abstract::To investigate the pharmacological mechanism of Guizhi Fuling Wan (GFW) in the treatment of uterine fibroids, a network pharmacology approach was used. Information on GFW compounds was collected from traditional Chinese medicine (TCM) databases, and input into PharmMapper to identify the compound targets. Genes associ...
journal_title:Experimental and therapeutic medicine
pub_type: 杂志文章
doi:10.3892/etm.2017.5170
更新日期:2017-11-01 00:00:00
abstract::Intrahepatic cholangiocarcinoma (ICC) is a cancer type with high malignancy and a current lack of biomarkers to predict recurrence. In the present study, to identify potential biomarkers, five ICC datasets from the Gene Expression Omnibus database were analyzed to construct initial datasets by using a robust rank aggr...
journal_title:Experimental and therapeutic medicine
pub_type: 杂志文章
doi:10.3892/etm.2020.9178
更新日期:2020-11-01 00:00:00
abstract::The aim of the present study was to evaluate the diagnostic value of ultrasound (US) elastography in differentiating between benign and malignant peripheral lung lesions (PLLs). This retrospective study included 91 consecutive patients with 91 PLLs. Conventional US, strain elastography (SE), acoustic radiation force i...
journal_title:Experimental and therapeutic medicine
pub_type: 杂志文章
doi:10.3892/etm.2018.6335
更新日期:2018-08-01 00:00:00
abstract::The aim of this study was to investigate the effects of heat stress on the expression of the coxsackievirus and adenovirus receptor (CAR) in mouse skin keratinocytes. Twenty BALB/c mice were randomly divided into two groups: the sham heat (control) and scald groups. Skin specimens were obtained 6 h after the treatment...
journal_title:Experimental and therapeutic medicine
pub_type: 杂志文章
doi:10.3892/etm.2013.1230
更新日期:2013-10-01 00:00:00
abstract::Tanshinone IIA (TSIIA) is a major component of Salvia miltiorrhiza, a Chinese herb that exhibits a therapeutic effect on polycystic ovary syndrome (PCOS). The present study replicated PCOS via the neonatal treatment of estradiol in mice. Estrous cycles, body and ovarian weight, serum levels of testosterone and estradi...
journal_title:Experimental and therapeutic medicine
pub_type: 杂志文章
doi:10.3892/etm.2019.7352
更新日期:2019-05-01 00:00:00
abstract::A severe immune response in patients with coronavirus disease 2019 (COVID-19) can cause a potentially lethal unconstrained inflammatory cytokine storm, known as cytokine release syndrome (CRS). The present study provides an overview of the biology underlying CRS and how targeted inhibition of interleukin (IL)-6 signal...
journal_title:Experimental and therapeutic medicine
pub_type: 杂志文章,评审
doi:10.3892/etm.2020.9456
更新日期:2021-01-01 00:00:00
abstract::The present study aimed to investigate whether c-mesenchymal epithelial transition factor (C-MET) overexpression combined with RAS (including KRAS, NRAS and HRAS) or BRAF mutations were associated with late distant metastases and the prognosis of patients with colorectal cancer (CRC). A total of 374 patients with stag...
journal_title:Experimental and therapeutic medicine
pub_type: 杂志文章
doi:10.3892/etm.2018.5966
更新日期:2018-05-01 00:00:00
abstract::The effects of different cytochrome P450 2C19 (CYP2C19) genotypes on the prognosis of clopidogrel resistance in patients complicated with atrial fibrillation taking clopidogrel after percutaneous coronary intervention (PCI) were investigated. Eighty patients who were complicated with atrial fibrillation and treated wi...
journal_title:Experimental and therapeutic medicine
pub_type: 杂志文章
doi:10.3892/etm.2018.6650
更新日期:2018-10-01 00:00:00
abstract::Ameloblastoma is a locally invasive benign odontogenic tumor with a high rate of recurrence in the long term. The authors conducted a retrospective study of patients with mandibular ameloblastoma in order to evaluate recurrent ameloblastoma management. The study included data from 31 patients over a period of 10 years...
journal_title:Experimental and therapeutic medicine
pub_type: 杂志文章
doi:10.3892/etm.2013.1165
更新日期:2013-08-01 00:00:00
abstract::Glucocorticoids are a class of steroid hormones commonly used for the treatment of hematological malignancies due to their ability to induce apoptosis in lymphoid cells. An understanding of the critical steps in glucocorticoid-induced apoptosis is required to identify sources of drug resistance. Previously, we found t...
journal_title:Experimental and therapeutic medicine
pub_type: 杂志文章
doi:10.3892/etm.2012.595
更新日期:2012-02-01 00:00:00
abstract::Expression levels and clinical significance of matrix metalloproteinase-1 (MMP1), MMP-2 and inflammatory factors in the serum of patients with deep venous thrombosis (DVT) of lower extremity were investigated. Fifty untreated DVT patients were selected as the DVT group, and 50 patients undergoing health examination we...
journal_title:Experimental and therapeutic medicine
pub_type: 杂志文章
doi:10.3892/etm.2018.6922
更新日期:2019-01-01 00:00:00
abstract::The present study aimed to evaluate whether thymosin α1 (Tα1) increases survival rates through the improvement of immunofunction and inhibition of hepatocyte apoptosis in rats with acute liver failure (ALF). A total of 25 rats were randomly divided into the control group (CG), the model group (MG) and the treatment gr...
journal_title:Experimental and therapeutic medicine
pub_type: 杂志文章
doi:10.3892/etm.2018.5843
更新日期:2018-04-01 00:00:00
abstract::Salivary gland malignant neoplasms (SGMNs) represent a group of malignant solid tumors with heterogeneity in their cellular make-up, which causes difficulty with regard to the immunohistochemical confirmation of their cytological features. In the present study, overexpression of the pituitary tumor transforming gene (...
journal_title:Experimental and therapeutic medicine
pub_type: 杂志文章
doi:10.3892/etm.2015.2566
更新日期:2015-08-01 00:00:00
abstract::Obesity and β-cell dysfunction due to oxidative stress impact the pathogenesis of type 2 diabetes mellitus. We co-cultured 3T3L1 adipocytes and islet cells in the presence or absence of the antioxidant α-lipoic acid (LA) and assayed the effects of the adipocytes and LA on the secretion of insulin by the islet cells an...
journal_title:Experimental and therapeutic medicine
pub_type: 杂志文章
doi:10.3892/etm.2012.601
更新日期:2012-09-01 00:00:00
abstract::Air pollution can highly impact the respiratory system in healthy individuals. Studies have indicated that particles with an aerodynamic diameter of ≤2.5 µm (PM2.5) can be considered to be harmful for lung alveoli and bronchial epithelium cells. PM2.5 can be directly inhaled and can deeply penetrate into the lung alve...
journal_title:Experimental and therapeutic medicine
pub_type: 杂志文章
doi:10.3892/etm.2020.9433
更新日期:2021-01-01 00:00:00
abstract::Paraquat (PQ) is a highly toxic herbicide, which not only leads to acute organ damage, but also to a variety of complications. Patients with severe PQ-induced poisoning may succumb to multiple organ failure involving the circulatory and respiratory systems. Although numerous studies have been performed investigating P...
journal_title:Experimental and therapeutic medicine
pub_type: 杂志文章
doi:10.3892/etm.2014.1944
更新日期:2014-11-01 00:00:00
abstract::Spinal cord injury (SCI) is a severe health problem and the mechanism involved remains elusive. The aim of the present study was to elucidate the role of C/EBP homologous protein (CHOP), a prominent protein of the endoplasmic reticulum (ER) stress-mediated apoptosis in SCI. A total of 20 adult male Sprague-Dawley rats...
journal_title:Experimental and therapeutic medicine
pub_type: 杂志文章
doi:10.3892/etm.2012.745
更新日期:2013-01-01 00:00:00
abstract::The C-X-C Motif Chemokine Receptor 4/C-X-C Motif Chemokine Ligand 12 (CXCR4/CXCL12) axis has been implicated in the pathogenesis of pulmonary fibrosis. However, the mechanisms governing this remain to be determined. The current study demonstrated that human lung fibroblasts (HLFs) exhibit high CXCL12 expression and al...
journal_title:Experimental and therapeutic medicine
pub_type: 杂志文章
doi:10.3892/etm.2020.8433
更新日期:2020-03-01 00:00:00
abstract::The aim of this study was to explore the effect of electroacupuncture (EA) on immune function in patients undergoing supratentorial craniotomy. We also examined whether point specificity in EA was present. The study involved 29 patients undergoing craniotomy. The patients were divided into three groups: a control (C, ...
journal_title:Experimental and therapeutic medicine
pub_type: 杂志文章
doi:10.3892/etm.2013.1225
更新日期:2013-09-01 00:00:00
abstract::The transient receptor potential melastatin 2 (TRPM2) channel, a Ca2+ permeable channel activated by cAMP, is expressed on pancreatic β-cells and is responsible for the regulation of insulin secretion. It is known that glucose-stimulated insulin secretion (GSIS) can be potentiated by glucagon like peptide-1 (GLP-1), a...
journal_title:Experimental and therapeutic medicine
pub_type: 杂志文章
doi:10.3892/etm.2017.5136
更新日期:2017-11-01 00:00:00
abstract::Homeobox D10 (HOXD10) belongs to the human homeobox (HOX) gene family, and the homologous protein encoded by HOX primarily controls cell differentiation and morphogenesis during embryonic development. The current study aimed to explore the roles and mechanisms of HOXD10 in the migration of human fibroblast-like synovi...
journal_title:Experimental and therapeutic medicine
pub_type: 杂志文章
doi:10.3892/etm.2018.6432
更新日期:2018-09-01 00:00:00
abstract::The present study aimed to analyze the imaging, clinical and pathological features of fungal infection involvement in primary biliary cirrhosis (PBC) by retrospectively analyzing and reviewing the features of two patients with fungal infection involvement in PBC. Both patients were female. One patient had a confirmed ...
journal_title:Experimental and therapeutic medicine
pub_type: 杂志文章
doi:10.3892/etm.2016.3977
更新日期:2017-02-01 00:00:00
abstract::Lung adenocarcinoma (LACA) is the leading cause of cancer-associated death worldwide. The present study intended to identify DNA methylation patterns that may serve as diagnostic and prognostic biomarkers for LACA. Data on DNA methylation and the survival data of the patients of LACA were obtained from The Cancer Geno...
journal_title:Experimental and therapeutic medicine
pub_type: 杂志文章
doi:10.3892/etm.2019.8200
更新日期:2020-01-01 00:00:00