Abstract:
:Epstein-Barr Virus (EBV) generates a variety of viral microRNAs (miRNAs) by processing the BHRF1 and BamHI A rightward (BART) transcripts. BART miRNAs are expressed in all cells latently infected with EBV, but the functions of most BART miRNAs remain unknown. The results of a cell proliferation assay revealed that miR-BART15-3p inhibited cell proliferation. Fluorescence-activated cell sorting following staining with annexin V or propidium iodide showed that miR-BART15-3p promoted apoptosis. Furthermore, the inhibitor for miR-BART15-3p increased cell growth and reduced apoptosis in EBV-infected cells. Using bioinformatic analyses, we predicted that miR-BART15-3p may target the antiapoptotic B-cell lymphoma 2 (BCL2), BCL2L2, DEAD (Asp-Glu-Ala-Asp) box polypeptide 42 (DDX42), and baculovirus inhibitor of apoptosis repeat-containing ubiquitin-conjugating enzyme (BRUCE) mRNAs. The luciferase reporter assay showed that only the 3' untranslated region (UTR) of BRUCE was affected by miR-BART15-3p. Two putative seed-matched sites for miR-BART15-3p were evident on the BRUCE 3' UTR. The results of a mutation study indicated that miR-BART15-3p hybridized only with the first seed-matched site on the BRUCE 3' UTR. miR-BART15-3p downregulated the BRUCE protein in EBV-negative cells, while the inhibitor for miR-BART15-3p upregulated the BRUCE protein in EBV-infected cells without affecting the BRUCE mRNA level. miR-BART15-3p was secreted from EBV-infected gastric carcinoma cells, and the level of miR-BART15-3p was 2- to 16-fold higher in exosomes than in the corresponding cells. Our data suggest that miR-BART15-3p can induce apoptosis partially by inhibiting the translation of the apoptosis inhibitor BRUCE. Further study is warranted to understand the role of miR-BART15-3p in the EBV life cycle.
journal_name
J Viroljournal_title
Journal of virologyauthors
Choi H,Lee H,Kim SR,Gho YS,Lee SKdoi
10.1128/JVI.03159-12subject
Has Abstractpub_date
2013-07-01 00:00:00pages
8135-44issue
14eissn
0022-538Xissn
1098-5514pii
JVI.03159-12journal_volume
87pub_type
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pub_type: 杂志文章
doi:10.1128/JVI.70.7.4538-4548.1996
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pub_type: 杂志文章
doi:10.1128/JVI.73.8.6293-6298.1999
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journal_title:Journal of virology
pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.4.962-971.1987
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.3.1809-1813.1992
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.2.834-839.1995
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pub_type: 杂志文章
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更新日期:2006-03-01 00:00:00