Detection and Genotyping Study of Enterocytozoon bieneusi in Sheep and Goats in East-central China.

Abstract:

BACKGROUND:Enterocytozoon bieneusi, the dominant zoonotic species of microsporidia, has been reported in various animals and humans. However, relatively little information is available concerning the worldwide prevalence and genotypes of E. bieneusi in ruminants, such as sheep and goats. The present study was conducted to evaluate the prevalence and genetic characteristics of E. bieneusi in sheep and goats in east-central China. METHODS:Fresh fecal samples from 832 sheep and 781 goats were evaluated for the presence of E. bieneusi using PCR and sequencing of the ribosomal internal transcribed spacer. RESULTS:28 sheep specimens (3.4%) and 32 goat specimens (4.1%) were positive for E. bieneusi. A total of 12 ITS genotypes were identified, including 7 known genotypes (COS-I, OEB1, BEB6, CHG1, CHG3, COS-II, and CHC8) and 5 novel genotypes (named AHS1, AHS2, JSS1, AHG1, and AHG2). The dominant genotypes in sheep and goats were BEB6 and CHG3, respectively. Phylogenetic analysis revealed that all genotypes obtained in this study belonged to the so-called "cattle-specific" Group 2. CONCLUSIONS:These findings show a relatively low occurrence and genetic diversity of E. bieneusi in sheep and goats in east-central China. Further studies are required to elucidate the role of sheep and goats in the epidemiology of microsporidia.

journal_name

Acta Parasitol

journal_title

Acta parasitologica

authors

Li WC,Wang K,Gu YF

doi

10.2478/s11686-018-00006-8

subject

Has Abstract

pub_date

2019-03-01 00:00:00

pages

44-50

issue

1

eissn

1230-2821

issn

1896-1851

pii

10.2478/s11686-018-00006-8

journal_volume

64

pub_type

杂志文章