Abstract:
INTRODUCTION:We define a fluid library as a library of samples of different biological fluids (from humans, animals or vectors) collected and properly stored on filter paper, which allows retrospective studies, especially of diagnosis or detection of infectious agents in these samples, using different techniques. The objective of this work was the retrospective diagnosis of American trypanosomiasis by PCR in a Venezuelan endemic area using a fluid library. METHODS:A fluid library with samples that had been collected on filter paper, 5 years ago, was used for the detection of Trypanosoma cruzi DNA. 165 blood samples of humans, 30 samples of 25 animals (Didelphis marsupialis, Canis familiaris, Equus asinus and Felis catus) and 8 samples of vectors from endemic areas of Anzoátegui state, were analysed by PCR. RESULTS:The results revealed that 16.4% of the humans samples were positive, 11.1% of those detected positive were children younger than 10 years old, and 26.72% young people aged 11-20 years, suggesting that T. cruzi infection has been active for the past two decades. 56% of the animal samples showed amplification; Didelphis marsupialis 66%, Canis familiaris 54.5%, Equus asinus 50%, and Felis catus 33.3%. On the other hand, positivity (50%) was detected in the studied vectors, of which the 3 most important species in Venezuela (Rhodnius prolixus, Triatoma maculata and Panstrongylus geniculatus) were involved. CONCLUSIONS:The PCR using a fluid library allowed the detection of T. cruzi DNA in old samples from the three host of the epidemiological chain, suggesting that retrospective diagnosis can be made through this strategy and demonstrate that there has been active transmission, which helps to clarify the epidemiological situation in areas where there are no previous reports.
journal_name
Acta Parasitoljournal_title
Acta parasitologicaauthors
López M,Herrera L,Morocoima A,Rivera MG,Viettri M,Lares M,Ferrer Edoi
10.1007/s11686-020-00281-4subject
Has Abstractpub_date
2020-09-15 00:00:00eissn
1230-2821issn
1896-1851pii
10.1007/s11686-020-00281-4pub_type
杂志文章abstract:INTRODUCTION:Alveolar echinococcosis (AE) is a zoonotic disease caused by the parasitism of Echinococcus multilocularis larvae in the intermediate host or the final host. This study aims to identify and analyze the B-cell and T-cell (Th1, Th2 and Th17) epitopes of E. multilocularis antigen Emy162. METHODS:(1) The seco...
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pub_type: 杂志文章
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更新日期:2020-12-01 00:00:00
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更新日期:2020-07-08 00:00:00
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更新日期:2016-04-01 00:00:00
abstract::Naturally Leishmania infantum infected bitches were divided into oestrogenized (n = 11) and non-oestrogenized (n = 6) groups. Vaginal secretions were collected for polymerase chain reaction (PCR), and vulval, vaginal and uterine tissues were collected for the immunohistochemical (IHQ) identification of L. infantum. Pa...
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pub_type: 杂志文章
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更新日期:2019-12-01 00:00:00
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pub_type: 杂志文章
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更新日期:2017-03-01 00:00:00
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doi:10.2478/s11686-014-0222-6
更新日期:2014-03-01 00:00:00
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pub_type: 杂志文章
doi:10.2478/s11686-012-0052-3
更新日期:2012-12-01 00:00:00
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pub_type: 杂志文章
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更新日期:2012-09-01 00:00:00
abstract::The Mascarene grass frog Ptychadena cf. mascareniensis is a species of frog with a vast area of distribution in Africa. A total of 300 frog specimens were collected from different localities at El-Giza province, Egypt; then dissected and examined for the presence of parasitic infection. Only eighty six (28.66%) specim...
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pub_type: 杂志文章
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更新日期:2017-06-01 00:00:00
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journal_title:Acta parasitologica
pub_type: 杂志文章
doi:10.1007/s11686-020-00311-1
更新日期:2020-11-22 00:00:00
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更新日期:2019-12-01 00:00:00
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pub_type: 杂志文章
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更新日期:2018-03-26 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:2013-03-01 00:00:00
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pub_type: 杂志文章
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更新日期:2014-09-01 00:00:00
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pub_type: 杂志文章
doi:10.1007/s11686-020-00247-6
更新日期:2020-07-15 00:00:00
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pub_type: 杂志文章
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更新日期:2012-03-01 00:00:00
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pub_type: 杂志文章
doi:10.1515/ap-2016-0084
更新日期:2016-09-01 00:00:00
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journal_title:Acta parasitologica
pub_type: 杂志文章
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更新日期:2019-09-01 00:00:00
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pub_type: 杂志文章
doi:10.1515/ap-2018-0068
更新日期:2018-09-25 00:00:00
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pub_type: 杂志文章,评审
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更新日期:2018-03-26 00:00:00
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journal_title:Acta parasitologica
pub_type: 杂志文章
doi:10.1515/ap-2017-0067
更新日期:2017-09-26 00:00:00
abstract::Cystic hydatidosis is a re-emerging parasitic zoonosis with worldwide distribution. The current study was carried out to investigate the possible role of rats in the epidemiology of such disease in urban and suburban areas. For this purpose, a total of 50 feral Norway rats (Rattus norvegicus) were collected from urban...
journal_title:Acta parasitologica
pub_type: 杂志文章
doi:10.1515/ap-2016-0113
更新日期:2016-12-01 00:00:00
abstract::Hookworms, a group to which Ancylostoma ceylanicum belongs, are gastrointestinal nematodes that infect more than 700 million people around the world. They are a leading cause of anemia in developing countries. In order to effectively prevent hookworm infections research is conducted to develop an effective vaccine usi...
journal_title:Acta parasitologica
pub_type: 杂志文章
doi:10.1515/ap-2016-0074
更新日期:2016-09-01 00:00:00
abstract:PURPOSE:Due to mass population movements driven by internal conflicts and wars, cutaneous leishmaniasis (CL) is becoming increasingly important in Turkey. This study is aimed at determining the clinical aspects, diagnosis and genotyping of CL patients coming to Turkey from abroad. METHODS:In our study, the clinical ma...
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pub_type: 杂志文章
doi:10.2478/s11686-019-00139-4
更新日期:2020-03-01 00:00:00