Abstract：
PURPOSE:To seek for the optimal immunization procedure of DNA vaccine pVAX1-pEtK2-IL-2 which was produced via cloning pEtK2 antigen gene of Eimeria tenella (E. tenella) and chicken IL-2 (chIL-2) gene into expression vector pVAX1. METHODS:The doses, routes, times of inoculation and ages of the first inoculation of chickens were optimized. The stability of the vaccine, including store temperature and time, was also explored. The effects of the protective immunity against challenge infection were assessed according to average body weight gain, survival rate, oocyst output, lesion score and the anti-coccidial index (ACI). RESULTS:The results suggested that intramuscular inoculation was the most efficient route to elicit immune response and 80 μg was the optimal immune dose. Two time injections induced more effective protection compared to single injection, the effect of the first injection at 14 days old was optimal. The immune efficacy of the vaccine stored at different time and temperature was very stable. CONCLUSIONS:The optimal immunization procedure for Eimeria tenella DNA vaccine pVAX1-pEtK2-IL-2 is 80 μg DNA, two time injections at 14 and 21 days old, respectively, by intramuscular inoculation.
journal_name
Acta Parasitoljournal_title
Acta parasitologicaauthors
Zhang B,Yuan C,Song X,Xu L,Yan R,Shah MAA,Guo C,Zhu S,Li Xdoi
10.2478/s11686-019-00090-4subject
Has Abstractpub_date
2019-12-01 00:00:00pages
745-752issue
4eissn
1230-2821issn
1896-1851pii
10.2478/s11686-019-00090-4journal_volume
64pub_type
杂志文章abstract：:A new species of Rhinonastes, hitherto monotypic, is described and illustrated from the nasal cavity of 'curimat', Prochilodus argenteus from São Francisco River, Brazil. Rhinonastes curimatae n. sp. presents a male copulatory organ with more than five rings while the type species of the genus is characterized by male...
journal_title：Acta parasitologica
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更新日期：2014-09-01 00:00:00
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更新日期：2013-03-01 00:00:00
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journal_title：Acta parasitologica
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更新日期：2015-06-01 00:00:00
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更新日期：2019-09-01 00:00:00
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更新日期：2020-06-01 00:00:00
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更新日期：2020-12-14 00:00:00
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更新日期：2015-09-01 00:00:00
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更新日期：2015-12-01 00:00:00
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更新日期：2016-01-01 00:00:00
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更新日期：2020-10-11 00:00:00
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pub_type： 杂志文章
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更新日期：2019-12-01 00:00:00
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pub_type： 杂志文章
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doi：10.2478/s11686-014-0244-0
更新日期：2014-06-01 00:00:00
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更新日期：2014-03-01 00:00:00
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更新日期：2016-12-01 00:00:00
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更新日期：2016-09-01 00:00:00
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更新日期：2013-12-01 00:00:00
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更新日期：2020-07-08 00:00:00
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更新日期：2014-09-01 00:00:00
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pub_type： 杂志文章
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更新日期：2020-09-15 00:00:00
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pub_type： 杂志文章
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更新日期：2012-12-01 00:00:00
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pub_type： 杂志文章
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更新日期：2016-03-01 00:00:00
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journal_title：Acta parasitologica
pub_type： 杂志文章
doi：10.2478/s11686-014-0281-8
更新日期：2014-10-01 00:00:00
