Abstract：
:In vivo imaging/spectroscopic biomarkers for solid tumor aggressiveness are needed in the clinic to facilitate cancer diagnosis and treatment strategies. In mouse models of human melanoma and breast cancer, we were able to detect the metabolic differences among tumors of different metastatic potential and between normal and cancer tissues by optical imaging of the mitochondrial redox state of snap-frozen tissue samples. Such metabolic differences indicate that tumors of different aggressiveness have different metabolic homeostasis, which supports that kinetic parameters such as rate constant(s) can also serve as biomarkers for cancer aggressiveness and treatment response. Here we present our preliminary study on the mouse xenografts of the aggressive and indolent human breast cancer cell lines using the hyperpolarized (13)C-NMR (HP-NMR) technique. By recording the time courses of (13)C-pyruvate tracer and its metabolite signals in vivo, particularly the (13)C-lactate signal, the apparent rate constants of both the forward and reverse reactions catalyzed by lactate dehydrogenase (LDH) were extracted via the ratiometric modeling of the two-site exchange reaction that we developed. Data from four breast tumors (MCF-7, MDA-MB-468, and MDA-MB-231 medium and large) with different aggressiveness are included. We demonstrate the feasibility to quantify the apparent rate constants of LDH reactions in breast tumor xenografts.
journal_name
Adv Exp Med Bioljournal_title
Advances in experimental medicine and biologyauthors
Xu HN,Kadlececk S,Pullinger B,Profka H,Cai K,Hariharan H,Rizi R,Li LZdoi
10.1007/978-1-4614-7411-1_32subject
Has Abstractpub_date
2013-01-01 00:00:00pages
237-242eissn
0065-2598issn
2214-8019journal_volume
789pub_type
杂志文章abstract：:1) Motility seems to be required for an intimate association with the intestinal mucosa which is necessary for efficient toxin delivery in the pathogenicity of V. cholerae. 2) A vaccine of semipurified flagella (CF) afforded a high degree of active and passive immunity in the rabbit ileum to both homologous and hetero...
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abstract：:Major congenital anomalies are detectable in 2-3 % of the newborn population. Some of their genetic causes are attributable to copy number variations identified by array comparative genomic hybridization (aCGH). The value of aCGH screening as a first-tier test in children with multiple congenital anomalies has been st...
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journal_title：Advances in experimental medicine and biology
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journal_title：Advances in experimental medicine and biology
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journal_title：Advances in experimental medicine and biology
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doi：10.1007/978-3-0348-0837-8_4
更新日期：2014-01-01 00:00:00
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journal_title：Advances in experimental medicine and biology
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doi：10.1007/978-0-387-39975-1_11
更新日期：2007-01-01 00:00:00
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journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
doi：10.1007/978-1-4419-5638-5_1
更新日期：2012-01-01 00:00:00
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journal_title：Advances in experimental medicine and biology
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journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：10.1007/978-1-4615-4863-8_81
更新日期：1998-01-01 00:00:00
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journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
doi：10.1007/978-81-322-1777-0_7
更新日期：2014-01-01 00:00:00
abstract：PURPOSE:To develop a reliable and efficient digital method to quantify planimetric Goldmann visual field (GVF) data to monitor disease course and treatment responses in retinal degenerative diseases. METHODS:A novel method to digitally quantify GVFs using Adobe Photoshop CS3 was developed for comparison to traditional...
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pub_type： 杂志文章
doi：10.1007/978-1-4614-3209-8_17
更新日期：2014-01-01 00:00:00
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journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
doi：10.1007/978-1-4757-5235-9_15
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journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
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journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
doi：10.1007/978-3-319-33826-2_4
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journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：10.1007/978-1-4684-3366-1_35
更新日期：1978-01-01 00:00:00
abstract：:We describe an investigation into how Massey University's Pollen Classifynder can accelerate the understanding of pollen and its role in nature. The Classifynder is an imaging microscopy system that can locate, image and classify slide based pollen samples. Given the laboriousness of purely manual image acquisition an...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：10.1007/978-3-319-10984-8_12
更新日期：2015-01-01 00:00:00
abstract：:Taurine release in mouse hippocampal slices is regulated by several neurotransmitter receptor systems. The ionotropic glutamate receptors and the adenosine receptor A(1) are the most effective. The effect of N-methyl-D-aspartate receptors is mediated via activation of the pathway involving nitric oxide and 3',5'-cycli...
journal_title：Advances in experimental medicine and biology
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doi：10.1007/978-1-4614-6130-2_11
更新日期：2013-01-01 00:00:00
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doi：10.1007/978-1-4615-0135-0_40
更新日期：2003-01-01 00:00:00
abstract：:Telocyte, different to fibroblast and dendritic cell, is a novel type of interstitial cell, whose key features are their smaller cell body with very long prolongations of uneven caliber, termed telopodes. The telocytes have been continuously discovered to be present in many tissues and organs. Whether telocytes exist ...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
doi：10.1007/978-981-10-1061-3_24
更新日期：2016-01-01 00:00:00
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journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：10.1007/978-1-4757-0731-1_37
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journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
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更新日期：2018-01-01 00:00:00
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journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
doi：10.1007/978-981-32-9705-0_12
更新日期：2020-01-01 00:00:00
abstract：:Monoclonal antibodies are universal binding molecules with a high specificity for their target and are indispensable tools in research, diagnostics and therapy. The biotechnological generation of monoclonal antibodies was enabled by the hybridoma technology published in 1975 by Köhler and Milstein. Today monoclonal an...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
doi：10.1007/978-3-319-32805-8_2
更新日期：2016-01-01 00:00:00
abstract：:Nuclear magnetic resonance (NMR) techniques are being increasingly utilised as an in vivo method to monitor tissue oxygen concentration in various organs. In muscle and heart, proton NMR spectroscopy of myoglobin has been used to calculate local oxygen tension through the oxygen sensitivity of the histidine group sign...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
doi：10.1007/978-1-4615-3404-4_22
更新日期：1992-01-01 00:00:00
