Abstract：
:The application of immobilized lipase in the enzymatic production of biodiesel has shown numerous advantages. In this study, surface of Polyacrylonitrile (PAN) hollow membrane was first modified using nitrile-click chemistry in order to fit for interaction with enzyme proteins. Then sodium alginate (SA) was introduced and the membrane was post-treated by CaCl2. When the prepared PAN-PEI-SA-CaCl2 was used for lipase immobilization, the protein loading was 36.90 mg/g, and the enzyme activity reached up to 54.47 U/g, which was 2.5 times as much as that of Novozym® 435. As a result, the constructed immobilized lipase obtained a maximum biodiesel yield of 78.5%, which was 2.4 times that of the Novozym® 435 in transesterification reactions. Moreover, the biodiesel yield decreased by only 11% after the immobilized enzyme was continuously used for 20 times. This study exhibits that this technic has broad application prospects in the field of conversion of biomass resources.
journal_name
Bioresour Technoljournal_title
Bioresource technologyauthors
Li Y,Wang H,Lu J,Chu A,Zhang L,Ding Z,Xu S,Gu Z,Shi Gdoi
10.1016/j.biortech.2018.11.075subject
Has Abstractpub_date
2019-02-01 00:00:00pages
9-17eissn
0960-8524issn
1873-2976pii
S0960-8524(18)31609-2journal_volume
274pub_type
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