Abstract：
:Uroporphyrinogen decarboxylase (Hem12p) and transketolase (Tkl1p) are key mediators of two critical processes within the cell, heme biosynthesis, and the nonoxidative part of the pentose phosphate pathway (PPP). The redox properties of both Hem12p and Tkl1p from Saccharomyces cerevisiae were investigated using proteomic techniques (SRM and label-free quantification) and biochemical assays in cell extracts and in vitro with recombinant proteins. The in vivo analysis revealed an increase in oxidized Cys-peptides in the absence of Grx2p, and also after treatment with H2O2 in the case of Tkl1p, without corresponding changes in total protein, demonstrating a true redox response. Out of three detectable Cys residues in Hem12p, only the conserved residue Cys52 could be modified by glutathione and efficiently deglutathionylated by Grx2p, suggesting a possible redox control mechanism for heme biosynthesis. On the other hand, Tkl1p activity was sensitive to thiol redox modification and although Cys622 could be glutathionylated to a limited extent, it was not a natural substrate of Grx2p. The human orthologues of both enzymes have been involved in certain cancers and possess Cys residues equivalent to those identified as redox sensitive in yeast. The possible implication for redox regulation in the context of tumour progression is put forward.
journal_name
Oxid Med Cell Longevjournal_title
Oxidative medicine and cellular longevityauthors
McDonagh B,Pedrajas JR,Padilla CA,Bárcena JAdoi
10.1155/2013/932472subject
Has Abstractpub_date
2013-01-01 00:00:00pages
932472eissn
1942-0900issn
1942-0994journal_volume
2013pub_type
杂志文章abstract：:Due to the high biotolerance, favourable mechanical properties, and osseointegration ability, titanium is the basic biomaterial used in maxillofacial surgery. The passive layer of titanium dioxide on the surface of the implant effectively provides anticorrosive properties, but it can be damaged, resulting in the relea...
journal_title：Oxidative medicine and cellular longevity
pub_type： 杂志文章
doi：10.1155/2018/3714725
更新日期：2018-06-14 00:00:00
abstract：:Aconitase, the second enzyme of the tricarboxylic acid cycle encoded by ACO1 in the budding yeast Saccharomyces cerevisiae, catalyzes the conversion of citrate to isocitrate. aco1Δ results in mitochondrial DNA (mtDNA) instability. It has been proposed that Aco1 binds to mtDNA and mediates its maintenance. Here we prop...
journal_title：Oxidative medicine and cellular longevity
pub_type： 杂志文章
doi：10.1155/2013/493536
更新日期：2013-01-01 00:00:00
abstract：:Age-related hearing loss (ARHL), the progressive loss of hearing associated with aging, is the most common sensory disorder in the elderly population. The pathology of ARHL includes the hair cells of the organ of Corti, stria vascularis, and afferent spiral ganglion neurons as well as the central auditory pathways. Ma...
journal_title：Oxidative medicine and cellular longevity
pub_type： 杂志文章,评审
doi：10.1155/2014/582849
更新日期：2014-01-01 00:00:00
abstract：:Hyperthyroidism is an endocrine disorder characterized by excessive secretion of thyroid hormones T3 and T4. Thyroid hormones exert pleiotropic actions on numerous tissues and induce an overall increase in metabolism, with an increase in energy demand and oxygen consumption. Therefore, the purpose of this study was to...
journal_title：Oxidative medicine and cellular longevity
pub_type： 杂志文章
doi：10.1155/2019/7471890
更新日期：2019-06-02 00:00:00
abstract：:The widely used herbicide atrazine (ATR) can cause many adverse effects including immunotoxicity, but the underlying mechanisms are not fully understood. The current study investigated the role of oxidative stress and calcium homeostasis in ATR-induced immunotoxicity in mice. ATR at doses of 0, 100, 200, or 400 mg/kg ...
journal_title：Oxidative medicine and cellular longevity
pub_type： 杂志文章
doi：10.1155/2016/7978219
更新日期：2016-01-01 00:00:00
abstract：:Oxidative stress has been proven as one of the most critical regulatory mechanisms involved in fine Particulate Matter- (PM2.5-) mediated toxicity. For a better understanding of the underlying mechanisms that enable oxidative stress to participate in PM2.5-induced toxic effects, the current study explored the effects ...
journal_title：Oxidative medicine and cellular longevity
pub_type： 杂志文章
doi：10.1155/2019/2492368
更新日期：2019-12-07 00:00:00
abstract：:Aluminum ions are especially toxic to plants in acidic soils. Here we present evidences that SO2 protects germinating wheat grains against aluminum stress. SO2 donor (NaHSO3/Na2SO3) pretreatment at 1.2 mM reduced the accumulation of superoxide anion, hydrogen peroxide, and malondialdehyde, enhanced the activities of g...
journal_title：Oxidative medicine and cellular longevity
pub_type： 杂志文章
doi：10.1155/2015/612363
更新日期：2015-01-01 00:00:00
abstract：:Ischemia-reperfusion (IR) injury is directly related to the formation of reactive oxygen species (ROS), endothelial cell injury, increased vascular permeability, and the activation of neutrophils and platelets, cytokines, and the complement system. Several studies have confirmed the destructiveness of the toxic oxygen...
journal_title：Oxidative medicine and cellular longevity
pub_type： 杂志文章,评审
doi：10.1155/2015/590987
更新日期：2015-01-01 00:00:00
abstract：:Diabetes is comorbid with cardiovascular disease and impaired immunity. Rapamycin improves cardiac functions and extends lifespan by inhibiting the mechanistic target of rapamycin complex 1 (mTORC1). However, in diabetic murine models, Rapamycin elevates hyperglycemia and reduces longevity. Since Rapamycin is an immun...
journal_title：Oxidative medicine and cellular longevity
pub_type： 杂志文章
doi：10.1155/2017/5724046
更新日期：2017-01-01 00:00:00
abstract：:We explored whether pu-erh tea consumption ameliorates atherosclerosis and the possible mechanism for its effects in apolipoprotein E-deficient (ApoE-/-) mice. Our data showed that pu-erh tea consumption markedly reduced early fatty streak formation and the advanced fibrofatty plaque sizes. Additionally, the mean prop...
journal_title：Oxidative medicine and cellular longevity
pub_type： 杂志文章
doi：10.1155/2018/3197829
更新日期：2018-08-23 00:00:00
abstract：:Tea polyphenols are of great benefit to the treatment of several neurodegenerative diseases. In order to explore the neuroprotective effects of tea polyphenols and their potential mechanisms, an established in vivo subarachnoid hemorrhage (SAH) model was used and alterations of mitochondrial function, ATP content, and...
journal_title：Oxidative medicine and cellular longevity
pub_type： 杂志文章
doi：10.1155/2013/743938
更新日期：2013-01-01 00:00:00
abstract：:Hypoxia-inducible gene domain family member 1A (Higd1a) has recently been reported to protect cells from hypoxia by helping to maintain normal mitochondrial function. The potential induction of Higd1a under high-fat exposure and whether it could protect cells from oxidative stress attracted our attention. Initially, 0...
journal_title：Oxidative medicine and cellular longevity
pub_type： 杂志文章
doi：10.1155/2019/6051262
更新日期：2019-04-08 00:00:00
abstract：Background:Acute kidney injury (AKI) is one of the common complications of sepsis. Heretofore, there is no effective treatment for septic AKI. Recent studies have revealed that besides treating hematological malignancies, human umbilical cord blood mononuclear cells (hUCBMNCs) show good therapeutic effects on other dis...
journal_title：Oxidative medicine and cellular longevity
pub_type： 杂志文章
doi：10.1155/2020/6123459
更新日期：2020-07-28 00:00:00
abstract：:Metabolic syndrome is becoming commoner due to a rise in obesity rates among adults. Generally speaking, a person with metabolic syndrome is twice as likely to develop cardiovascular disease and five times as likely to develop diabetes as someone without metabolic syndrome. Increasing oxidative stress in metabolic syn...
journal_title：Oxidative medicine and cellular longevity
pub_type： 杂志文章,评审
doi：10.1155/2014/729194
更新日期：2014-01-01 00:00:00
abstract：:Renal ischemia-reperfusion injury (RIRI) refers to a phenomenon associated with dysfunction of the kidney and tissue damage. Unfortunately, no specific drugs have been found that effectively prevent and treat RIRI. Curcumin (Cur), a polyphenol extracted from turmeric, possesses a variety of biological activities invol...
journal_title：Oxidative medicine and cellular longevity
pub_type： 杂志文章
doi：10.1155/2020/4546851
更新日期：2020-11-12 00:00:00
abstract：:The aim of this study was to evaluate the properties of internal components of erythrocytes in chronic renal failure (CRF) patients undergoing hemodialysis (HD) in comparison to control subjects. For investigation of conformational state of hemoglobin and nonheme proteins (NHP) the maleimide spin label (MSL) in electr...
journal_title：Oxidative medicine and cellular longevity
pub_type： 杂志文章
doi：10.1155/2015/783073
更新日期：2015-01-01 00:00:00
abstract：:An imbalance in the reactive oxygen species (ROS) homeostasis is involved in the pathogenesis of oxidative stress-related diseases. Astaxanthin, a xanthophyll carotenoid with high antioxidant capacities, has been shown to prevent the first stages of oxidative stress. Here, we evaluate the antioxidant capacities of ast...
journal_title：Oxidative medicine and cellular longevity
pub_type： 杂志文章
doi：10.1155/2017/8073798
更新日期：2017-01-01 00:00:00
abstract：:Idiopathic pulmonary fibrosis (IPF) is a progressive, irreversible lung disease of unknown etiology with limited survival. IPF incidence and prevalence increase significantly with aging, which is associated with an age-related accumulation of oxidative DNA damage. The Mutyh gene is involved in the base excision repair...
journal_title：Oxidative medicine and cellular longevity
pub_type： 杂志文章
doi：10.1155/2020/4828256
更新日期：2020-10-16 00:00:00
abstract：:There is accumulating evidence that blood pressure (BP) control significantly reduces the risk of future cardiovascular events in patients with essential hypertension. However, strict BP control is often difficult to maintain, and half of hypertensive patients fail to attain BP goals on single-drug therapy. Therefore,...
journal_title：Oxidative medicine and cellular longevity
pub_type： 杂志文章
doi：10.4161/oxim.3.5.13199
更新日期：2010-09-01 00:00:00
abstract：:Copper, a transition metal with essential cellular functions, exerts neurotoxic effects when present in excess by promoting production of reactive oxygen species (ROS). The aim of the present study was to investigate potential benefits of flavonoid quercetin against copper-induced toxicity. Results obtained with MTT a...
journal_title：Oxidative medicine and cellular longevity
pub_type： 杂志文章
doi：10.1155/2020/9834742
更新日期：2020-07-11 00:00:00
abstract：:Oxidative stress (OS), a common intracellular phenomenon induced by excess reactive oxygen species (ROS) generation, has been shown to be associated with mammalian ovarian follicular development blockage and granulosa cell (GC) impairment. However, the mechanism involved in these effects remains unknown, and the effec...
journal_title：Oxidative medicine and cellular longevity
pub_type： 杂志文章
doi：10.1155/2019/1041583
更新日期：2019-11-04 00:00:00
abstract：:Hyperglycemia-induced stress in the brain of patients with diabetes triggers the disruption of blood-brain barrier (BBB), leading to diverse neurological diseases including stroke and dementia. Recently, the role of microRNA becomes an interest in the research for deciphering the mechanism of brain endothelial cell da...
journal_title：Oxidative medicine and cellular longevity
pub_type： 杂志文章
doi：10.1155/2017/6051874
更新日期：2017-01-01 00:00:00
abstract：:Acute pancreatitis (AP) is a multifactorial disease characterized by necroinflammatory changes of the pancreas. Our study is the first study which evaluated the relationship between the free radical production, enzymatic and nonenzymatic antioxidants, oxidative damage, and secretory function of the salivary glands of ...
journal_title：Oxidative medicine and cellular longevity
pub_type： 杂志文章
doi：10.1155/2019/8403578
更新日期：2019-03-10 00:00:00
abstract：:Diabetes is a disease that can be treated with oral antidiabetic agents and/or insulin. However, patients' metabolic control is inadequate in a high percentage of them and a major cause of chronic diseases like diabetic retinopathy. Approximately 15% of patients have some degree of diabetic retinopathy when diabetes i...
journal_title：Oxidative medicine and cellular longevity
pub_type： 杂志文章,评审
doi：10.1155/2019/4940825
更新日期：2019-11-11 00:00:00
abstract：:Alzheimer's disease (AD) is the most common form of dementia, characterized by progressive neurodegeneration. Pathogenetic mechanisms, triggered by β-amyloid (Aβ) accumulation, include oxidative stress, derived from energy homeostasis deregulation and involving mitochondria and peroxisomes. We here addressed the oxida...
journal_title：Oxidative medicine and cellular longevity
pub_type： 杂志文章
doi：10.1155/2015/967203
更新日期：2015-01-01 00:00:00
abstract：:We recently showed that combination therapy with losartan and pioglitazone provided synergistic effects compared with monotherapy in improving lesions of renal structure and function in Sprague-Dawley rats fed with a high-fat, high-sodium diet and 20% sucrose solution. This study was designed to explore the underlying...
journal_title：Oxidative medicine and cellular longevity
pub_type： 杂志文章
doi：10.1155/2012/856085
更新日期：2012-01-01 00:00:00
abstract：:Mitochondria are the main organelles that produce adenosine 5'-triphosphate (ATP) and reactive oxygen species (ROS) in eukaryotic cells and meanwhile susceptible to oxidative damage. The irreversible oxidative damage in mitochondria has been implicated in various human diseases. Increasing evidence indicates the thera...
journal_title：Oxidative medicine and cellular longevity
pub_type： 杂志文章,评审
doi：10.1155/2020/8837893
更新日期：2020-12-03 00:00:00
abstract：OBJECTIVE:To explore the protective effects of hydrogen-rich saline on rats with smoke inhalation injury. METHODS:36 healthy male Sprague-Dawley rats were randomly divided into 3 groups (n = 12 per group): sham group (S), inhalation injury plus normal saline treatment group (I+NS), and inhalation injury plus hydrogen-...
journal_title：Oxidative medicine and cellular longevity
pub_type： 杂志文章
doi：10.1155/2015/106836
更新日期：2015-01-01 00:00:00
abstract：:Sarcopenia is the loss of muscle mass accompanied by a decrease in muscle strength and resistance and is the main cause of disability among the elderly. Muscle loss begins long before there is any clear physical impact in the senior adult. Despite all this, the molecular mechanisms underlying muscle aging are far from...
journal_title：Oxidative medicine and cellular longevity
pub_type： 杂志文章,评审
doi：10.1155/2016/9057593
更新日期：2016-01-01 00:00:00
abstract：:Chronic unpredictable mild stress (CUMS) induces hippocampal oxidative stress. H2S functions as a neuroprotectant against oxidative stress in brain. We have previously shown the upregulatory effect of H2S on BDNF protein expression in the hippocampus of rats. Therefore, we hypothesized that H2S prevents CUMS-generated...
journal_title：Oxidative medicine and cellular longevity
pub_type： 杂志文章
doi：10.1155/2016/2153745
更新日期：2016-01-01 00:00:00
