Evaluation of amniotic fluid alpha-fetoprotein using an enzyme-linked immunosorbent assay.

Abstract:

:A simple, inexpensive enzyme-linked immunosorbent assay using commercially available supplies is described. An examination of amniotic fluid alpha-fetoprotein (AFP) values for 15 to 20 weeks' gestation is presented. The interassay coefficient of variation was 12% (N = 40) at a level of 4.75 micrograms/mL. Repeat freezing, thawing, and storage at 4C for three months had no discernible effect on measured AFP value. Data comparison with a radioimmunoassay (RIA) kit indicates similar reliability for both assays. There were no false-negative values (zero of 20). The false-positive rate for RIA was 4.4% (13 of 295) and for enzyme-linked immunosorbent assay was 5.0% (15 of 295).

journal_name

Obstet Gynecol

authors

Bogart MH,Winkelbauer D,Jones OW

subject

Has Abstract

pub_date

1985-09-01 00:00:00

pages

341-5

issue

3

eissn

0029-7844

issn

1873-233X

journal_volume

66

pub_type

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