Abstract:
:Polycarbonate (PC) substrate is well suited for culturing human mesenchymal stem cells (MSCs) with high proliferation rate, low cell apoptosis rate and negligible cytotoxic effects. However, little is known about the influence of PC on MSC activity including senescence, differentiation and secretion. In this study, the PC cell culture insert was applied for human MSC culture and was compared with polystyrene (PS) and standard tissue culture plate (TCP). The results showed that MSCs were able to adhere on PC surface, exhibiting a spindle-shaped morphology. The size and distribution of focal adhesions of MSCs were similar on PC and TCP. The senescence level of MSCs on PC was comparable to that on TCP, but was significantly lower than that on PS. MSCs on PC were capable of self-renewal and differentiation into multiple cell lineages, including osteogenic and adipogenic lineages. MSCs cultured on PC secreted a higher level inflammatory cytokines and pro-angiogenic factors including FGF2 and VEGF. Conclusively, PC represents a promising cell culture material for human MSCs.
journal_name
Clin Hemorheol Microcircjournal_title
Clinical hemorheology and microcirculationauthors
Zou J,Wang W,Kratz K,Xu X,Nie Y,Ma N,Lendlein Adoi
10.3233/CH-189322subject
Has Abstractpub_date
2018-01-01 00:00:00pages
573-583issue
4eissn
1386-0291issn
1875-8622pii
CH189322journal_volume
70pub_type
杂志文章abstract:PURPOSE:Cross limb stent graft (SG) configuration technique for endovascular aneurysm repair (EVAR) is employed for splayed aortic bifurcations to avoid device kinking and smoothen cannulation. The present study investigates three types of stent graft (SG) configurations for endovascular aneurysm repair (EVAR) in abdom...
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journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章,评审
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journal_title:Clinical hemorheology and microcirculation
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journal_title:Clinical hemorheology and microcirculation
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journal_title:Clinical hemorheology and microcirculation
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journal_title:Clinical hemorheology and microcirculation
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journal_title:Clinical hemorheology and microcirculation
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journal_title:Clinical hemorheology and microcirculation
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doi:
更新日期:2008-01-01 00:00:00
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journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:
更新日期:2008-01-01 00:00:00
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journal_title:Clinical hemorheology and microcirculation
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doi:
更新日期:2000-01-01 00:00:00
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journal_title:Clinical hemorheology and microcirculation
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更新日期:2003-01-01 00:00:00
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journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:
更新日期:2002-01-01 00:00:00
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journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:10.3233/CH-131729
更新日期:2013-01-01 00:00:00
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journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:
更新日期:2008-01-01 00:00:00
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journal_title:Clinical hemorheology and microcirculation
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doi:10.3233/CH-2010-1367
更新日期:2011-01-01 00:00:00
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journal_title:Clinical hemorheology and microcirculation
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doi:10.3233/CH-16196
更新日期:2017-01-01 00:00:00
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journal_title:Clinical hemorheology and microcirculation
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doi:10.3233/CH-2011-1459
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journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:
更新日期:2006-01-01 00:00:00
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journal_title:Clinical hemorheology and microcirculation
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更新日期:2015-01-01 00:00:00
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更新日期:2016-01-01 00:00:00
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journal_title:Clinical hemorheology and microcirculation
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journal_title:Clinical hemorheology and microcirculation
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journal_title:Clinical hemorheology and microcirculation
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更新日期:2018-01-01 00:00:00
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journal_title:Clinical hemorheology and microcirculation
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