Abstract：
OBJECTIVES:This study aimed to explore the genetic diversity of Streptococcus pneumoniae isolates in a Tunisian pneumology hospital. METHODS:A total of 141 S. pneumoniae strains isolated between 2009-2016 in the microbiology laboratory at A. Mami Hospital of Pneumology were investigated. Antimicrobial susceptibility testing was performed the disk diffusion method. MICs of penicillin G, amoxicillin and cefotaxime were determined by Etest. Serotyping was inferred from the results of multiplex PCR targeting 40 serotypes. Sequence types (STs) were determined by multilocus sequence typing (MLST). RESULTS:Among the 141 S. pneumoniae isolates, 98 (69.5%) were resistant to erythromycin. Evaluation of β-lactam susceptibility showed that 90 strains (63.8%) were non-susceptible to penicillin, whereas 48 (34.0%) had decreased susceptibility to amoxicillin and 21 (14.9%) to cefotaxime. Twenty-five serotypes were detected, and 10 isolates were classified as non-typeable. Vaccine coverage was 56.7%, 60.3% and 75.2% for pneumococcal conjugate vaccine 7 (PCV7), PCV10 and PCV13, respectively. Overall, 73 STs were identified, including 23 described for the first time. The most frequent STs were ST179 (n = 17), ST3772 (n = 14), ST2918 (n = 10) and ST4003 (n = 5), related to serotypes 19F, 19A, 14 and 23F, respectively. Moreover, 110 strains were classified within 45 STs. Three international antimicrobial-resistant clones were found, including Denmark14-ST230 (n = 22), Spain9V-ST156 (n = 22) and Portugal19F-ST177 (n = 20). CONCLUSION:This study emphasises the clonal and international dissemination of antimicrobial-resistant S. pneumoniae clones. Significant differences in genetic variation were documented by MLST within the various serotypes identified.
journal_name
Int J Antimicrob Agentsjournal_title
International journal of antimicrobial agentsauthors
Midouni B,Mehiri E,Ghariani A,Draoui H,Essalah L,Bouzouita I,Raoult D,Slim-Saidi L,Fournier PEdoi
10.1016/j.ijantimicag.2018.09.023subject
Has Abstractpub_date
2019-01-01 00:00:00pages
63-69issue
1eissn
0924-8579issn
1872-7913pii
S0924-8579(18)30282-6journal_volume
53pub_type
杂志文章abstract：:Seven new sites leading to increased beta-lactam susceptibility were identified and mapped in Staphylococcus aureus by Tn551 or Tn917 mediated insertional inactivation. Their effect was more pronounced in methicillin resistant than in susceptible strains. Except for inserts each in SmaI-K and SmaI-B, all were located ...
journal_title：International journal of antimicrobial agents
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journal_title：International journal of antimicrobial agents
pub_type： 杂志文章,评审
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journal_title：International journal of antimicrobial agents
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journal_title：International journal of antimicrobial agents
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journal_title：International journal of antimicrobial agents
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journal_title：International journal of antimicrobial agents
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journal_title：International journal of antimicrobial agents
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journal_title：International journal of antimicrobial agents
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journal_title：International journal of antimicrobial agents
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journal_title：International journal of antimicrobial agents
pub_type： 杂志文章,评审
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journal_title：International journal of antimicrobial agents
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journal_title：International journal of antimicrobial agents
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journal_title：International journal of antimicrobial agents
pub_type： 杂志文章
doi：10.1016/j.ijantimicag.2012.05.023
更新日期：2012-10-01 00:00:00
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journal_title：International journal of antimicrobial agents
pub_type： 杂志文章,评审
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journal_title：International journal of antimicrobial agents
pub_type： 杂志文章
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更新日期：2007-10-01 00:00:00
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journal_title：International journal of antimicrobial agents
pub_type： 杂志文章
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journal_title：International journal of antimicrobial agents
pub_type： 杂志文章
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journal_title：International journal of antimicrobial agents
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journal_title：International journal of antimicrobial agents
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journal_title：International journal of antimicrobial agents
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journal_title：International journal of antimicrobial agents
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journal_title：International journal of antimicrobial agents
pub_type： 杂志文章,多中心研究
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journal_title：International journal of antimicrobial agents
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更新日期：2021-01-01 00:00:00
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