Isolation and quantitation of isopentenyladenosine in an anise cell culture by single-ion monitoring, radioimmunoassay and bioassay.

Abstract:

:Using three different techniques, isopentenyladenosine was identified and quantitated in an anise cell line (Pimpinella anisum L.), growing on a medium without cytokinin. A method to quantitate cytokinins was developed which utilizes extraction of cytokinins in the presence of a deuterated reference compound and subsequent quantitation of the cytokinins by single-ion monitoring on a gas chromatography-mass spectrometry system. The results were compared with those obtained by radioimmunoassay as well as a bioassay. Quantitations by gas chromatography-mass spectrometry and radioimmunoassay correlated well, whereas the estimates by the bioassay gave considerably lower values.

journal_name

Planta

journal_title

Planta

authors

Ernst D,Schäfer W,Oesterhelt D

doi

10.1007/BF00397527

subject

Has Abstract

pub_date

1983-11-01 00:00:00

pages

216-21

issue

3

eissn

0032-0935

issn

1432-2048

journal_volume

159

pub_type

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