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Molecular diagnosis of Arcobacter and Campylobacter in diarrhoeal samples among Portuguese patients.

Abstract：

:The present study was conducted to investigate the prevalence and diversity of Arcobacter and Campylobacter spp. in 298 stool samples of patients with diarrhoea, collected from 22 Portuguese hospitals, between September and November 2012. Detection of Arcobacter and Campylobacter spp. was performed using molecular-based detection techniques, such as real-time fluorescence resonance energy transfer PCR, species-specific PCR, and sequencing of amplified PCR products. Overall, 1.3% of the samples were positive for Arcobacter butzleri and 0.3% for Arcobacter cryaerophilus. Campylobacter spp. were found in 31.9% of diarrhoeic faeces. Campylobacter jejuni and Campylobacter concisus were the most prevalent species (13.7% and 8.0%, respectively). The prevalence of Arcobacter and Campylobacter spp. was significantly different between children and adults (39.7% versus 22.8%, P = 0.003). We underline the high prevalence of these pathogens in diarrhoeal samples among Portuguese patients, with particular relevance in the paediatric age group.

journal_name

Diagn Microbiol Infect Dis

journal_title

Diagnostic microbiology and infectious disease

authors

Ferreira S,Júlio C,Queiroz JA,Domingues FC,Oleastro M

doi

10.1016/j.diagmicrobio.2013.11.021

subject

Has Abstract

pub_date

2014-03-01 00:00:00

pages

220-5

issue

3

eissn

0732-8893

issn

1879-0070

pii

S0732-8893(13)00634-2

journal_volume

78

pub_type

杂志文章,多中心研究

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