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Determination of Tumor Margins with Surgical Specimen Mapping Using Near-Infrared Fluorescence.

Abstract：

:For many solid tumors, surgical resection remains the gold standard and tumor-involved margins are associated with poor clinical outcomes. Near-infrared (NIR) fluorescence imaging using molecular agents has shown promise for in situ imaging during resection. However, for cancers with difficult imaging conditions, surgical value may lie in tumor mapping of surgical specimens. We thus evaluated a novel approach for real-time, intraoperative tumor margin assessment. Twenty-one adult patients with biopsy-confirmed squamous cell carcinoma arising from the head and neck (HNSCC) scheduled for standard-of-care surgery were enrolled. Cohort 1 (n = 3) received panitumumab-IRDye800CW at an intravenous microdose of 0.06 mg/kg, cohort 2A (n = 5) received 0.5 mg/kg, cohort 2B (n = 7) received 1 mg/kg, and cohort 3 (n = 6) received 50 mg. Patients were followed 30 days postinfusion and adverse events were recorded. Imaging was performed using several closed- and wide-field devices. Fluorescence was histologically correlated to determine sensitivity and specificity. In situ imaging demonstrated tumor-to-background ratio (TBR) of 2 to 3, compared with ex vivo specimen imaging TBR of 5 to 6. We obtained clear differentiation between tumor and normal tissue, with a 3-fold signal difference between positive and negative specimens (P < 0.05). We achieved high correlation of fluorescence intensity with tumor location with sensitivities and specificities >89%; fluorescence predicted distance of tumor tissue to the cut surface of the specimen. This novel method of detecting tumor-involved margins in surgical specimens using a cancer-specific agent provides highly sensitive and specific, real-time, intraoperative surgical navigation in resections with complex anatomy, which are otherwise less amenable to image guidance.Significance: This study demonstrates that fluorescence can be used as a sensitive and specific method of guiding surgeries for head and neck cancers and potentially other cancers with challenging imaging conditions, increasing the probability of complete resections and improving oncologic outcomes. Cancer Res; 78(17); 5144-54. ©2018 AACR.

journal_name

Cancer Res

journal_title

Cancer research

authors

Gao RW,Teraphongphom NT,van den Berg NS,Martin BA,Oberhelman NJ,Divi V,Kaplan MJ,Hong SS,Lu G,Ertsey R,Tummers WSFJ,Gomez AJ,Holsinger FC,Kong CS,Colevas AD,Warram JM,Rosenthal EL

doi

10.1158/0008-5472.CAN-18-0878

subject

Has Abstract

pub_date

2018-09-01 00:00:00

pages

5144-5154

issue

eissn

0008-5472

issn

1538-7445

pii

0008-5472.CAN-18-0878

journal_volume

pub_type

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Determination of Tumor Margins with Surgical Specimen Mapping Using Near-Infrared Fluorescence.