Immunobead-PCR: a technique for the detection of circulating tumor cells using immunomagnetic beads and the polymerase chain reaction.

Abstract:

:The presence of tumor cells in the circulation may predict disease recurrence and metastases. We have developed a sensitive technique for the detection of carcinoma cells in blood, using immunomagnetic beads to enrich for epithelial cells and the polymerase chain reaction to identify a tumor marker. The colon carcinoma cell line SW480, homozygous for a K-ras codon 12 mutation, was used to establish optimal conditions. The SW480 cells were serially diluted in normal blood and incubated with immunomagnetic beads labeled with a monoclonal antibody specific for epithelial cells. Cells bound to the beads were retrieved using a magnetic field and the presence of K-ras codon 12 mutations determined by a polymerase chain reaction based analysis. SW480 cells could be detected in dilutions up to 1 SW480 cell/10(5) leukocytes in whole blood.

journal_name

Cancer Res

journal_title

Cancer research

authors

Hardingham JE,Kotasek D,Farmer B,Butler RN,Mi JX,Sage RE,Dobrovic A

subject

Has Abstract

pub_date

1993-08-01 00:00:00

pages

3455-8

issue

15

eissn

0008-5472

issn

1538-7445

journal_volume

53

pub_type

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