Abstract:
:Although curcumin was widely applied as a functional food for different diseases, it was found to reduce serum testosterone level and fertility in male animals by unknown molecular mechanisms. Here in our study, we investigated the possible mechanisms of curcumin-suppressed testosterone production in Leydig cells. Our enzyme immunoassay results showed that curcumin cell-autonomously suppressed ovine luteinizing hormone-stimulated testosterone production in primary Leydig cells and 8-bromo-cyclic adenosine monophosphate (8-br-cAMP)-induced progesterone production in MA-10 cells. Furthermore, our real-time PCR, Western blot, and 22R-OHC/pregnenolone supplementing experiment data demonstrated that curcumin suppressed 8-br-cAMP-induced steroidogenesis in Leydig cells by inhibiting the expression of StAR and Cyp11a1. Interestingly, our Western blot data showed that although curcumin suppressed PKA activity, it did not alter the 8-br-cAMP-induced phosphorylation of CREB. On the contrary, the real-time PCR results showed that curcumin suppressed 8-br-cAMP-induced expression of Nr5a1 and Fos, which are crucial for cAMP-stimulated StAR and Cyp11a1 expression in Leydig cells. Collectively, our data demonstrated that curcumin may suppress cAMP-induced steroidogenesis in mouse Leydig cells by down-regulating Nr5a1/Fos-controlled StAR and Cyp11a1 expression independently of the PKA-CREB signaling pathway.
journal_name
Endocr Jjournal_title
Endocrine journalauthors
Lin YC,Chiu CH,Liu HC,Wang JYdoi
10.1507/endocrj.EJ18-0010subject
Has Abstractpub_date
2018-08-27 00:00:00pages
833-840issue
8eissn
0918-8959issn
1348-4540journal_volume
65pub_type
杂志文章abstract::In the last ten years a liquid formulation of liothyronine (L-T3) became available. To date, no studies on its systematic use have been reported. This study is aimed at assessing the reliability of liquid L-T3 in achieving target TSH in patients with differentiated thyroid cancers (DTC). Twenty-one high risk DTC patie...
journal_title:Endocrine journal
pub_type: 临床试验,杂志文章
doi:10.1507/endocrj.EJ16-0040
更新日期:2016-06-30 00:00:00
abstract::Medullary thyroid carcinoma (MTC) may mimic mixed medullary and follicular thyroid carcinoma (MMFTC). MTC originates from para-follicular cells, while MMFTC is an uncommon tumor characterized by coexistence of follicular and para-follicular cell-derived tumor populations. A 35-year-old woman was diagnosed with MTC but...
journal_title:Endocrine journal
pub_type: 杂志文章
doi:10.1507/endocrj.EJ20-0169
更新日期:2020-10-28 00:00:00
abstract::A 67-year-old female with hypertension and impaired glucose tolerance was admitted to our hospital because of a typical acromegalic appearance, including large, thickened bulky hands and feet, and a large prominent forehead and tongue. She did not have a Cushingoid appearance, such as a moon-face, buffalo hump, purple...
journal_title:Endocrine journal
pub_type: 杂志文章
doi:10.1507/endocrj.ej14-0093
更新日期:2014-01-01 00:00:00
abstract::Endothelial dysfunction is considered to be an early event in the development of atherosclerosis. The present study was undertaken to evaluate endothelial function and biochemical markers in type 2 diabetes mellitus (T2DM) patients before and after treatment with or without pioglitazone (PIO). Forty-one T2DM patients ...
journal_title:Endocrine journal
pub_type: 杂志文章
doi:10.1507/endocrj.k08e-308
更新日期:2009-01-01 00:00:00
abstract::Pathogenesis of pseudohypoparathyroidism type 1b (PHP-1b) is related to the loss of methylation at the GNAS exon A/B region, which is combined with epigenetic defects at other differentially methylated GNAS regions in most sporadic cases. In this study, we established a method for evaluating the methylation status of...
journal_title:Endocrine journal
pub_type: 杂志文章
doi:10.1507/endocrj.k10e-364
更新日期:2011-01-01 00:00:00
abstract:OBJECTIVE:The purpose of this study was to assess the rate of malignancy in thyroid nodules incidentally detected at sonography and to determine the diagnostic value of ultrasonography-guided fine needle aspiration cytology (USgFNAC) in thyroid nodules. METHODS:Five hundred patients (84 men and 416 women) who had thyr...
journal_title:Endocrine journal
pub_type: 杂志文章
doi:10.1507/endocrj.k07-120
更新日期:2008-03-01 00:00:00
abstract::We previously developed a radioreceptor assay which is presumably specific for detection of blocking-type anti-TSH receptor (TSHR) antibodies using unsolubilized porcine TSHR. Employing this assay, we measured blocking TSH-binding inhibitory immunoglobulin (TBII) in the sera from 30 untreated Graves' patients and comp...
journal_title:Endocrine journal
pub_type: 杂志文章
doi:10.1507/endocrj.48.703
更新日期:2001-12-01 00:00:00
abstract::Estrogen has been shown to stimulate lactotroph proliferation and expression of the prolactin (PRL) gene. Recently it has been established that Pit-1, a pituitary-specific transcription factor, is required for lactotroph proliferation. Furthermore, in vitro studies showed that an increase in the PRL promoter activity ...
journal_title:Endocrine journal
pub_type: 杂志文章
doi:10.1507/endocrj.41.579
更新日期:1994-10-01 00:00:00
abstract::In this study, we investigated the difference in lymph node-recurrence free survival (LN-RFS), distant recurrence-free survival (DRFS), and cause-specific survival (CSS) between patients with papillary thyroid carcinoma (PTC) in the entire group (Group I) and those with lymph node- and distant-recurrence-free survival...
journal_title:Endocrine journal
pub_type: 杂志文章
doi:10.1507/endocrj.ej12-0176
更新日期:2012-01-01 00:00:00
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journal_title:Endocrine journal
pub_type: 杂志文章
doi:10.1507/endocrj.k09e-140
更新日期:2009-01-01 00:00:00
abstract::The tissue-specific circulating markers of thyroid hormone action on cardiac function have not been established. Although the relationship between thyroid function and plasma brain natriuretic peptide (BNP) levels has been evaluated in patients with thyroid disorders, the relationship between these parameters in the g...
journal_title:Endocrine journal
pub_type: 杂志文章
doi:10.1507/endocrj.EJ19-0380
更新日期:2020-04-28 00:00:00
abstract::Insulin-like growth factor-I activates a calcium-permeable cation channel GRC (growth factor-regulated channel). In the present study, we investigated the immunohistochemical localization of GRC in human tissues using a polyclonal anti-GRC antibody. Immunoreactive GRC was detected in the stomach, duodenum, large intes...
journal_title:Endocrine journal
pub_type: 杂志文章
doi:10.1507/endocrj.49.349
更新日期:2002-06-01 00:00:00
abstract::Non-invasive follicular thyroid neoplasm with papillary-like nuclear features (NIFTP) and invasive encapsulated follicular variant of papillary thyroid carcinoma (EFV-PTC) are indistinguishable preoperatively. CD26 expression in follicular tumor-uncertain malignant potential (FT-UMP) is reported to be clearly higher t...
journal_title:Endocrine journal
pub_type: 杂志文章
doi:10.1507/endocrj.EJ19-0501
更新日期:2020-12-28 00:00:00
abstract::Irisin is an adipokine/myokine which could be connected with insulin sensitivity. Polycystic ovary syndrome (PCOS) is characterized by oligo- or anovulation, polycystic ovary, hyperandrogenism and insulin resistance. The aim of the present study was to determine the relationship between serum irisin concentration and ...
journal_title:Endocrine journal
pub_type: 杂志文章
doi:10.1507/endocrj.EJ16-0249
更新日期:2016-12-30 00:00:00
abstract::Continuous subcutaneous insulin infusion (CSII) therapy using insulin pumps has become widely used in the treatment of type 1 diabetes mellitus (T1DM). This retrospective study aimed to assess the efficacy and safety of long-term insulin pump treatment in patients with T1DM aged ≥50 years. The study included patients ...
journal_title:Endocrine journal
pub_type: 杂志文章
doi:10.1507/endocrj.EJ19-0188
更新日期:2020-03-28 00:00:00
abstract::Exercise is a fundamental component of diabetes management. However, choosing inappropriate type or timing of exercise is associated with mild or severe hypoglycemia either during exercise or several hours after exercise. Several studies have shown that impaired counterregulatory responses triggers hypoglycemia. There...
journal_title:Endocrine journal
pub_type: 杂志文章,评审
doi:10.1507/endocrj.EJ19-0153
更新日期:2019-09-28 00:00:00
abstract::We report herein the case of a 1-year-old boy with McCune-Albright syndrome (MAS) who presented with infantile-onset Cushing' s syndrome caused by ACTH independent macronodular adrenal hyperplasia (AIMAH). Abdominal CT, MRI, and adrenal scintigraphy with (131)I-adosterol identified bilateral adrenal involvement with t...
journal_title:Endocrine journal
pub_type: 杂志文章
doi:10.1507/endocrj.k10e-003
更新日期:2010-01-01 00:00:00
abstract::In this study, nine patients with Graves' ophthalmopathy with positive clinical activity score (CAS), who were either unresponsive or not suitable for glucocorticoid treatment, were given 100 microg of octreotide three times daily, subcutaneously, for three months. The mean age was 49+/-13 years. All patients were und...
journal_title:Endocrine journal
pub_type: 杂志文章
doi:10.1507/endocrj.46.573
更新日期:1999-08-01 00:00:00
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journal_title:Endocrine journal
pub_type: 杂志文章
doi:10.1507/endocrj.ej14-0330
更新日期:2014-01-01 00:00:00
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journal_title:Endocrine journal
pub_type: 杂志文章,meta分析
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更新日期:2018-04-26 00:00:00
abstract::A preoperative molecular-based diagnostic technique for thyroid papillary and anaplastic carcinomas was recently developed to detect oncofetal fibronectin (onfFN) messenger RNA (mRNA) in fine needle aspiration biopsy (FNAB) by means of reverse transcription-polymerase chain reaction (RT-PCR). We report two cases of th...
journal_title:Endocrine journal
pub_type: 杂志文章
doi:10.1507/endocrj.47.271
更新日期:2000-06-01 00:00:00
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journal_title:Endocrine journal
pub_type: 杂志文章
doi:10.1507/endocrj.52.353
更新日期:2005-06-01 00:00:00
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journal_title:Endocrine journal
pub_type: 杂志文章,评审
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更新日期:2014-01-01 00:00:00
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journal_title:Endocrine journal
pub_type: 杂志文章,meta分析
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更新日期:2014-01-01 00:00:00
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journal_title:Endocrine journal
pub_type: 杂志文章
doi:10.1507/endocrj.EJ20-0123
更新日期:2020-11-28 00:00:00
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journal_title:Endocrine journal
pub_type: 杂志文章
doi:10.1507/endocrj.EJ18-0352
更新日期:2019-03-28 00:00:00
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journal_title:Endocrine journal
pub_type: 杂志文章
doi:10.1507/endocrj.EJ15-0480
更新日期:2016-04-25 00:00:00
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journal_title:Endocrine journal
pub_type: 杂志文章
doi:10.1507/endocrj.50.697
更新日期:2003-12-01 00:00:00
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journal_title:Endocrine journal
pub_type: 杂志文章
doi:10.1507/endocrj.EJ16-0494
更新日期:2017-06-29 00:00:00
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journal_title:Endocrine journal
pub_type: 杂志文章
doi:10.1507/endocrj.45.609
更新日期:1998-10-01 00:00:00