Molecular identification of the agent of Q fever - Coxiella burnetii - in domestic animals in State of Rio de Janeiro, Brazil.

Abstract:

INTRODUCTION:Over the last recent years, the number of Q fever cases have has increased throughout the world. An epidemiological investigation was performed in the area in which the first molecular documentation of Q fever in Brazil was previously reported. METHODS:Indirect immunofluorescence assay (IFA) and PCR of Coxiella burnetii targeting the htpAB gene were performed in samples from 14 dogs (blood); 1 cat (blood); 10 goats (blood, milk, vaginal swab and anal swab); 3 sheep (blood); and 2 horses (blood). RESULTS:Two dogs, two sheep and five goats were seroreactive. DNA was amplified from 6 milk and 2 blood samples from goats and from dogs, respectively. The sequence of the amplicons exhibited 99% sequence similarity with the homologous sequence of the htpAB gene of C. burnetii RSA 331 (GenBank - CP000890). CONCLUSIONS:The results confirm C. burnetii infection in animals in Rio de Janeiro and reinforce the need for the surveillance of Q fever in Brazil.

journal_name

Rev Soc Bras Med Trop

authors

Mares-Guia MA,Rozental T,Guterres A,Gomes R,Almeida DN,Moreira NS,Barreira JD,Favacho AR,Santana AL,Lemos ER

doi

10.1590/0037-8682-0076-2013

subject

Has Abstract

pub_date

2014-03-01 00:00:00

pages

231-4

issue

2

eissn

0037-8682

issn

1678-9849

pii

S0037-86822014000200231

journal_volume

47

pub_type

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