Abstract：
:The development of a novel enzyme-linked immunosorbent assay (ELISA) for determining luteinizing hormone (LH) in bovine plasma is described. Anti-bovine LH (bLH) monoclonal antibodies (mAbs) were produced and characterized. One mAb recognizing the bLH β subunit was used for immunoaffinity purification of substantial amounts of biologically active bLH from pituitary glands. The purified bLH in combination with 2 anti-bLH β subunit mAbs was used to develop a sandwich ELISA, which satisfied all the criteria required to investigate LH secretory patterns in the bovine species. The ELISA standard curve was linear over the range 0.05 to 2.5 ng/mL, and the assay proved suitable for measuring bLH in plasma without any prior treatment of samples. Cross-reactivity and recovery tests confirmed the specificity of the method. The intra- and inter-assay coefficients of variation ranged between 3.41% and 9.40%, and 9.29% and 15.84%, respectively. The analytical specificity of the method was validated in vivo by provocative tests for LH in heifers, using the LH releasing peptide gonadotropin-releasing hormone. In conclusion, the adoption of mAbs for this ELISA for coating the wells and labeling, combined with the easy one-step production of reference bLH, ensures long-term continuity in large-scale measurements of LH in the bovine species.
journal_name
Domest Anim Endocrinoljournal_title
Domestic animal endocrinologyauthors
Borromeo V,Berrini A,De Grandi F,Cremonesi F,Fiandanese N,Pocar P,Secchi Cdoi
10.1016/j.domaniend.2014.03.004subject
Has Abstractpub_date
2014-07-01 00:00:00pages
145-57eissn
0739-7240issn
1879-0054pii
S0739-7240(14)00034-4journal_volume
48pub_type
杂志文章abstract：:Curcumin, the active component of curcuma longa, has been reported to be effective in alleviating chronic stress-induced disorders in rodents by modulating neuroprotection and neuroendocrine functions of the central nervous system, especially hippocampus. However, it is unclear whether curcumin can attenuate the subac...
journal_title：Domestic animal endocrinology
pub_type： 杂志文章
doi：10.1016/j.domaniend.2010.06.004
更新日期：2010-11-01 00:00:00
abstract：:Reduced reproductive performance of lactating cows is strongly associated with environmental and pathogenic stressors. This review summarizes the most recent knowledge on the effects of acute or chronic heat stress (HS) and acute or chronic intramammary infection (IMI) on ovarian function. It also offers various appro...
journal_title：Domestic animal endocrinology
pub_type： 杂志文章,评审
doi：10.1016/j.domaniend.2016.02.013
更新日期：2016-07-01 00:00:00
abstract：:Corpus luteum (CL), a transient endocrine gland critical for reproductive cyclicity and pregnancy maintenance, is controlled by numerous regulatory factors. Although LH is widely recognized as the major regulator, other factors may also affect luteal functions. It has been demonstrated that FSH receptors (FSHR) are ex...
journal_title：Domestic animal endocrinology
pub_type： 杂志文章
doi：10.1016/j.domaniend.2019.106391
更新日期：2020-04-01 00:00:00
abstract：:Prolonged hyperinsulinemia is thought to be the cause of equine endocrinopathic laminitis, a common and crippling disease of the foot, for which there are no pharmacologic treatments other than pain relief. It has been suggested that insulin causes its effects on the lamellae by activating IGF-1 receptors (IGF-1R), as...
journal_title：Domestic animal endocrinology
pub_type： 杂志文章
doi：10.1016/j.domaniend.2020.106510
更新日期：2021-01-01 00:00:00
abstract：:Zymosan is a cell wall component of the yeast Saccharomyces cerevisiae and produces severe inflammatory responses in mammals. When zymosan is peripherally injected in mammals, it induces several behavioral and physiological changes including anorexia and hyperthermia. However, to our knowledge, behavioral and physiolo...
journal_title：Domestic animal endocrinology
pub_type： 杂志文章
doi：10.1016/j.domaniend.2020.106471
更新日期：2020-07-01 00:00:00
abstract：:The aim of this study was to determine the effects of growth hormone (GH) and insulin-like growth factor (IGF)-I on glycerol release and the regulation of IGF-I and IGF-II expression by GH in isolated rainbow trout adipocytes. Cells were also incubated with GH, tumor necrosis factor α (TNFα), or insulin to analyze the...
journal_title：Domestic animal endocrinology
pub_type： 杂志文章
doi：10.1016/j.domaniend.2014.11.002
更新日期：2015-04-01 00:00:00
abstract：:Experiments were conducted to determine if a nocturnal rise in serum melatonin occurs in prepubertal gilts and whether acute exposure of gilts to light during the dark period abruptly reduces serum concentrations of melatonin. In experiment 1, 12 prepubertal crossbred gilts (Duroc x Hampshire x Chester White x Yorkshi...
journal_title：Domestic animal endocrinology
pub_type： 杂志文章
doi：10.1016/0739-7240(92)90029-w
更新日期：1992-04-01 00:00:00
abstract：:Bile acids (BAs) are synthesized in the liver via the oxidation of cholesterol and further metabolized by microbiota in the gut, where they simultaneously impact gut function. In the present study, 10 goats were randomly divided into 2 groups; 1 group was injected with dexamethasone (Dex; 0.2 mg/kg), and the other gro...
journal_title：Domestic animal endocrinology
pub_type： 杂志文章
doi：10.1016/j.domaniend.2018.05.005
更新日期：2018-10-01 00:00:00
abstract：:Equine pituitary pars intermedia dysfunction (PPID) has been associated with reduced insulin sensitivity in comparison with younger adult horses; however, the difference in insulin sensitivity between horses with PPID and aged-matched controls has not been well characterized. The objective of the study was to determin...
journal_title：Domestic animal endocrinology
pub_type： 杂志文章
doi：10.1016/j.domaniend.2014.07.003
更新日期：2015-01-01 00:00:00
abstract：:An attenuated ovulatory rise in circulating concentrations of LH is characteristically associated with the first seasonal reproductive cycle of horse mares. Unlike ovulations (OV) of subsequent estrous cycles, the first OV of the breeding season (OV1) is not preceded by elevated concentrations of progesterone (PROG). ...
journal_title：Domestic animal endocrinology
pub_type： 杂志文章
doi：10.1016/0739-7240(91)90061-n
更新日期：1991-04-01 00:00:00
abstract：:This study was performed to determine whether in dogs with pituitary-dependent hyperadrenocorticism (PDH) excessive release of adrenocorticotrophic hormone (ACTH) is accompanied by secretion of ACTH precursor molecules. In addition, we investigated whether the plasma ACTH precursor concentrations were correlated with ...
journal_title：Domestic animal endocrinology
pub_type： 杂志文章
doi：10.1016/s0739-7240(02)00128-5
更新日期：2002-06-01 00:00:00
abstract：:The relationship between in vitro somatotroph function and growth was examined in piglets demonstrating a continuous range of growth characteristics. Twenty barrows were sacrificed at 3 weeks of age for the collection of pituitary tissue and blood. Pituitary cells from each animal were cultured and exposed to vehicle ...
journal_title：Domestic animal endocrinology
pub_type： 杂志文章
doi：10.1016/0739-7240(94)90008-6
更新日期：1994-10-01 00:00:00
abstract：:Water buffaloes are easily adaptable animals, whose raising and economical exploitation have been growing in the last three decades all over the world. Hyperstimulation of ovarian function in this species is a common technique aiming to improve reproductive performance. Superovulatory treatment affects corpus luteum (...
journal_title：Domestic animal endocrinology
pub_type： 杂志文章
doi：10.1016/j.domaniend.2006.07.003
更新日期：2007-11-01 00:00:00
abstract：:Considering that recumbency can lead to a change in localization, detectability of distinct margins and thus measurement of the organs and reconstruction quality, it is of value to evaluate the effect of positioning on the objective evaluation of abdominal organs in computed tomography (CT). The objective of the prese...
journal_title：Domestic animal endocrinology
pub_type： 杂志文章
doi：10.1016/j.domaniend.2019.106406
更新日期：2020-07-01 00:00:00
abstract：:Crossbred ovariectomized ewes were treated with steroid therapies determined previously to be adequate (progesterone-primed) or inadequate (unprimed) for embryonic development in order to determine actual serum concentrations of replaced steroid hormones achieved by such treatments and to identify secreted endometrial...
journal_title：Domestic animal endocrinology
pub_type： 杂志文章
doi：10.1016/0739-7240(93)90017-6
更新日期：1993-04-01 00:00:00
abstract：:Preliminary data suggest that prohormone convertase 1 (PC1/3) protein expression and proopiomelanocortin (POMC) processing are altered in large corticotroph tumors. The aim of this study was to characterize the levels of ACTH precursors and PC1/3 protein in small and large corticotroph tumors of dogs with Cushing's di...
journal_title：Domestic animal endocrinology
pub_type： 杂志文章
doi：10.1016/j.domaniend.2017.10.001
更新日期：2018-01-01 00:00:00
abstract：:Tyrosine aminotransferase (TAT) catalyzes the transamination of tyrosine to p-hydroxyphenylpyruvate. Accumulation of tyrosine in the body due to a genetic mutation in the TAT gene causes tyrosomia type II in humans. The TAT gene is regarded as a model for studying steroid-inducible factors regulating a variety of biol...
journal_title：Domestic animal endocrinology
pub_type： 杂志文章
doi：10.1016/j.domaniend.2016.05.001
更新日期：2016-10-01 00:00:00
abstract：:The site within the hypothalamic-pituitary axis at which cortisol acts to inhibit luteinizing hormone (LH) secretion was investigated in female pigs. Six ovariectomized, hypophysial stalk-transected (HST) gilts were given 1 microgram pulses of gonadotropin releasing-hormone (GnRH) iv every 45 min from day 0 to 12. On ...
journal_title：Domestic animal endocrinology
pub_type： 杂志文章
doi：10.1016/0739-7240(91)90008-8
更新日期：1991-07-01 00:00:00
abstract：:Effects of domperidone, a peripheral dopamine receptor antagonist, on secretion of LH and prolactin were studied during the luteal phase and following administration of PGF2 alpha. Since hyperprolactinemia has been reported to inhibit secretion of LH in ewes, effects of thyrotropin-releasing hormone (TRH) also were ex...
journal_title：Domestic animal endocrinology
pub_type： 杂志文章
doi：10.1016/0739-7240(87)90003-8
更新日期：1987-04-01 00:00:00
abstract：:The objective of this study was to characterize plasma insulin-like growth factor-I (IGF-I) profiles during the estrous cycle in goats. Frequent blood samples were drawn during the day of estrus and during the luteal phase on Day 10 after estrus, and plasma growth hormone (GH) and IGF-I profiles were examined. Then, d...
journal_title：Domestic animal endocrinology
pub_type： 杂志文章
doi：10.1016/s0739-7240(99)00082-x
更新日期：2000-02-01 00:00:00
abstract：:The objective of the present study was to determine if dexamethasone (Dex; a potent synthetic glucocorticoid) treatment at birth would alter postnatal growth in neonatal pigs. Forty crossbred pigs were injected i.m. with either sterile saline (Cont; n = 10 males and 10 females) or Dex (1 mg/kg; n = 10 males and 10 fem...
journal_title：Domestic animal endocrinology
pub_type： 杂志文章
doi：10.1016/s0739-7240(01)00107-2
更新日期：2001-08-01 00:00:00
abstract：:This study was aimed at testing the hypothesis that insulin-like growth factor binding protein (IGFBP)-3 can modulate hormone-dependent differentiation of granulosa cells in vitro. Granulosa cells from small (1 to 5 mm) follicles were collected from cattle, cultured for 2 d in medium containing 10% fetal calf serum, w...
journal_title：Domestic animal endocrinology
pub_type： 杂志文章
doi：10.1016/s0739-7240(98)00049-6
更新日期：1999-01-01 00:00:00
abstract：:In this study, the expression patterns of inhibins, activins, insulin-like growth factor-I (IGF-I) and steroidogenic enzymes in equine placentae recovered during the latter two-thirds of gestation were examined. Concentrations of inhibin A and inhibin pro-alphaC in endometrial and fetal placental tissue homogenates we...
journal_title：Domestic animal endocrinology
pub_type： 杂志文章
doi：10.1016/j.domaniend.2005.09.005
更新日期：2006-07-01 00:00:00
abstract：:The regional distribution and relative concentrations of LHRH and beta-endorphin immunoreactivity in the hypothalamus were compared in sexually active and sexually inactive Soay rams. LHRH was localized principally in the medial basal hypothalamus and median eminence with lower concentrations in the anterior hypothala...
journal_title：Domestic animal endocrinology
pub_type： 杂志文章
doi：10.1016/0739-7240(87)90010-5
更新日期：1987-07-01 00:00:00
abstract：:Assay of blood progesterone (P4) is commonly practiced to determine the time of ovulation, diagnose luteal insufficiency, and predict time of parturition in bitches. Because of practical constraints, most blood samples cannot be assayed on site immediately after collection. The aim of this work was to evaluate the eff...
journal_title：Domestic animal endocrinology
pub_type： 杂志文章
doi：10.1016/j.domaniend.2013.07.002
更新日期：2013-10-01 00:00:00
abstract：:Age-dependent interactions between environmental temperature and porcine growth hormone (pGH) treatment on the function of the somatotrophic axis were evaluated in the neonatal pig. At 3 d of age, 40 Landrace x Yorkshire x Duroc piglets received intraperitoneal implants containing either recombinant pGH (0.5 mg/d; n =...
journal_title：Domestic animal endocrinology
pub_type： 杂志文章
doi：10.1016/s0739-7240(99)00003-x
更新日期：1999-02-01 00:00:00
abstract：:The influence of phthalate esters di-2-ethylhexyl phthalate (DEHP) and mono-2-ethylhexyl phthalate (MEHP) on uterine prostaglandin (PGF2alpha and PGE2) and ovarian oxytocin secretion was investigated. Endometrial, granulosa, and luteal cells from cows on days 8-12 of the estrous cycle were treated with DEHP or MEHP (0...
journal_title：Domestic animal endocrinology
pub_type： 杂志文章
doi：10.1016/j.domaniend.2010.03.002
更新日期：2010-08-01 00:00:00
abstract：:The objective of transgenic livestock improvement projects is to develop and bring to market superior breeding stock, as well as germplasm for the artificial insemination and embryo transfer industries. Livestock animal biotechnology programs hold the promise of achieving, in a single generation, improvements in comme...
journal_title：Domestic animal endocrinology
pub_type： 杂志文章,评审
doi：10.1016/0739-7240(90)90049-6
更新日期：1990-01-01 00:00:00
abstract：:Muscle-targeted gene therapy using insulin genes has the potential to provide an inexpensive, low maintenance alternative or adjunctive treatment method for canine diabetes mellitus. A canine skeletal muscle cell line was established through primary culture, as well as through transdifferentiation of canine fibroblast...
journal_title：Domestic animal endocrinology
pub_type： 杂志文章
doi：10.1016/j.domaniend.2012.01.006
更新日期：2012-07-01 00:00:00
abstract：:The phenomenon of nuclear translocation of growth hormone receptor (GHR) in human, rat, and fish has been reported. To date, this phenomenon has not been described in a domestic animal (such as pig). In addition, the molecular mechanisms of GHR nuclear translocation have not been thoroughly elucidated. To this end, po...
journal_title：Domestic animal endocrinology
pub_type： 杂志文章
doi：10.1016/j.domaniend.2017.05.003
更新日期：2017-10-01 00:00:00
