Abstract:
:Equine pituitary pars intermedia dysfunction (PPID) has been associated with reduced insulin sensitivity in comparison with younger adult horses; however, the difference in insulin sensitivity between horses with PPID and aged-matched controls has not been well characterized. The objective of the study was to determine if aged horses with PPID had reduced insulin sensitivity and alterations in the insulin-mediated signaling pathways in the skeletal muscle when compared with healthy aged horses. Isoglycemic hyperinsulinemic clamp procedures were conducted in 12 horses that were classified as either PPID (n = 6; age: 25.0 ± 2.5 yr; mean ± standard deviation) or non-PPID, aged-matched controls (control) (n = 6; age: 25.7 ± 2.0 yr). Blood samples were taken before and during the clamp procedures to measure plasma glucose, insulin, and amino acid concentrations, and 2 muscle biopsies were collected from the gluteus medius muscle, one in the basal state and the second at the end of the clamp procedure (insulin-stimulated state). Plasma insulin concentrations increased ∼9-fold during the clamp compared with basal conditions (P < 0.001) in both groups. During the last 30 min of the clamp, the rate of glucose infusion required to maintain isoglycemia in horses with PPID was similar to that in the control horses (P = 0.67). The plasma concentrations of most indispensible amino acids were lower in the insulin-stimulated state than the basal state (P < 0.05). PPID status did not have an effect on the activation of factors associated with protein synthesis and breakdown; however, factors associated with protein synthesis had increased phosphorylation in the insulin-stimulated state, compared with basal. The results from this study provide evidence that PPID is not always associated with impairments in insulin sensitivity.
journal_name
Domest Anim Endocrinoljournal_title
Domestic animal endocrinologyauthors
Mastro LM,Adams AA,Urschel KLdoi
10.1016/j.domaniend.2014.07.003subject
Has Abstractpub_date
2015-01-01 00:00:00pages
14-25eissn
0739-7240issn
1879-0054pii
S0739-7240(14)00068-Xjournal_volume
50pub_type
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journal_title:Domestic animal endocrinology
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journal_title:Domestic animal endocrinology
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journal_title:Domestic animal endocrinology
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journal_title:Domestic animal endocrinology
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journal_title:Domestic animal endocrinology
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journal_title:Domestic animal endocrinology
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journal_title:Domestic animal endocrinology
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journal_title:Domestic animal endocrinology
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journal_title:Domestic animal endocrinology
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journal_title:Domestic animal endocrinology
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journal_title:Domestic animal endocrinology
pub_type: 杂志文章
doi:10.1016/0739-7240(93)90024-6
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journal_title:Domestic animal endocrinology
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journal_title:Domestic animal endocrinology
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journal_title:Domestic animal endocrinology
pub_type: 杂志文章
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journal_title:Domestic animal endocrinology
pub_type: 杂志文章
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journal_title:Domestic animal endocrinology
pub_type: 杂志文章
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journal_title:Domestic animal endocrinology
pub_type: 杂志文章,评审
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journal_title:Domestic animal endocrinology
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journal_title:Domestic animal endocrinology
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abstract::Mammary gland growth and morphogenesis are regulated by interactions between hormones as much as by their individual actions. The effect of these interactions on the mammary gland phenotype in species other than rodents is relatively undefined. We investigated the individual and combined effects of estrogen (E), proge...
journal_title:Domestic animal endocrinology
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abstract::Experimentally induced fever is accompanied by a variety of hormonal changes, and there is evidence to suggest that some of these responses may be mediated by prostaglandins. However, little is known about the endocrine effects of peripherally administered prostaglandins, especially in domesticated species. In this st...
journal_title:Domestic animal endocrinology
pub_type: 杂志文章
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