Abstract:
:It has now been well documented that the normal and tumorous canine mammary glands can be extra-pituitary sites of substantial growth hormone (GH) synthesis. Until now, attempts to reproduce the GH synthesis in-vitro using canine mammary explants or mammary tumor cells have not been successful. Therefore, the response of CMT-U335 canine mammary tumor cells to administered porcine GH (pGH) was investigated as an in-vitro model to study the possible effects of GH synthesis on this site. CMT-U335 cells spontaneously express the growth hormone receptor (GHR) as well as the prolactin receptor (PRLR). Twenty five minutes after administration, GH induced, in a dose-dependent manner, phosphorylation of the transcription factors Stat5a and Stat5b. Clear phosphorylation was induced by 10(-7) M and 10(-8) M pGH, with virtually no phosphorylation at 10(-9) M pGH. A similar dose-dependent phosphorylation of Stat5a by ovine prolactin was found in these cells. Although at high concentrations binding of pGH to the canine PRLR can occur (albeit with a low pKa), the similar dose-dependent effect of oPRL on Stat5a phosphorylation indicated that pGH signaled through the GHR. Remarkably, pGH induced a moderately decreased proliferation of CMT-U335 tumor cells, which may indicate that GH induces differentiation in these tumor cells. The GH-induced activation of Stat5a and Stat5b in these cells, as part of the JAK/Stat signal transduction pathway, is consistent with mammary GH playing a role in autocrine and/or paracrine stimulation of (tumorous) mammary cells.
journal_name
Domest Anim Endocrinoljournal_title
Domestic animal endocrinologyauthors
van Garderen E,Swennenhuis JF,Hellmén E,Schalken JAdoi
10.1016/s0739-7240(01)00088-1subject
Has Abstractpub_date
2001-02-01 00:00:00pages
123-35issue
2eissn
0739-7240issn
1879-0054pii
S0739-7240(01)00088-1journal_volume
20pub_type
杂志文章abstract::The flash glucose monitoring system (FGMS) Freestyle Libre provides estimates of blood glucose by constantly measuring the glucose concentration of the interstitial fluid through a sensor inserted in the subcutaneous space. The aim of this study was to evaluate the applicability and accuracy of the FGMS in dogs with d...
journal_title:Domestic animal endocrinology
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journal_title:Domestic animal endocrinology
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doi:10.1016/s0739-7240(97)00057-x
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journal_title:Domestic animal endocrinology
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journal_title:Domestic animal endocrinology
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journal_title:Domestic animal endocrinology
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doi:10.1016/j.domaniend.2020.106447
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journal_title:Domestic animal endocrinology
pub_type: 杂志文章,评审
doi:10.1016/s0739-7240(01)00120-5
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journal_title:Domestic animal endocrinology
pub_type: 杂志文章
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journal_title:Domestic animal endocrinology
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journal_title:Domestic animal endocrinology
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journal_title:Domestic animal endocrinology
pub_type: 杂志文章
doi:10.1016/j.domaniend.2013.01.005
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journal_title:Domestic animal endocrinology
pub_type: 杂志文章
doi:10.1016/j.domaniend.2003.08.004
更新日期:2004-01-01 00:00:00
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journal_title:Domestic animal endocrinology
pub_type: 杂志文章,评审
doi:10.1016/s0739-7240(98)00015-0
更新日期:1998-09-01 00:00:00
abstract::Age-dependent interactions between environmental temperature and porcine growth hormone (pGH) treatment on the function of the somatotrophic axis were evaluated in the neonatal pig. At 3 d of age, 40 Landrace x Yorkshire x Duroc piglets received intraperitoneal implants containing either recombinant pGH (0.5 mg/d; n =...
journal_title:Domestic animal endocrinology
pub_type: 杂志文章
doi:10.1016/s0739-7240(99)00003-x
更新日期:1999-02-01 00:00:00
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journal_title:Domestic animal endocrinology
pub_type: 杂志文章
doi:10.1016/s0739-7240(99)00053-3
更新日期:1999-11-01 00:00:00
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journal_title:Domestic animal endocrinology
pub_type: 杂志文章
doi:10.1016/j.domaniend.2016.07.004
更新日期:2017-01-01 00:00:00
abstract::Receptors for LH (LHR) and FSH (FSHR) are expressed in the canine lower urinary tract (LUT). As gonadectomy results in an increase in plasma LH and FSH, the objective of this study was to determine whether there are any differences in the expression of LHR and FSHR in the LUT between intact and gonadectomised dogs. Fo...
journal_title:Domestic animal endocrinology
pub_type: 杂志文章
doi:10.1016/j.domaniend.2007.09.005
更新日期:2008-05-01 00:00:00
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journal_title:Domestic animal endocrinology
pub_type: 杂志文章
doi:10.1016/j.domaniend.2004.01.007
更新日期:2004-07-01 00:00:00
abstract::Dogs do not appear to progress from obesity-induced insulin resistance to type 2 diabetes mellitus. Both postprandial hyperglycemia and postprandial hypertriglyceridemia have been proposed to cause or maintain beta cell failure and progression to type 2 diabetes mellitus in other species. Postprandial glucose, triglyc...
journal_title:Domestic animal endocrinology
pub_type: 杂志文章
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journal_title:Domestic animal endocrinology
pub_type: 杂志文章
doi:10.1016/j.domaniend.2004.04.002
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journal_title:Domestic animal endocrinology
pub_type: 杂志文章
doi:10.1016/j.domaniend.2005.10.002
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journal_title:Domestic animal endocrinology
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journal_title:Domestic animal endocrinology
pub_type: 杂志文章
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journal_title:Domestic animal endocrinology
pub_type: 杂志文章
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更新日期:2005-04-01 00:00:00
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journal_title:Domestic animal endocrinology
pub_type: 杂志文章
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journal_title:Domestic animal endocrinology
pub_type: 杂志文章
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更新日期:1991-04-01 00:00:00
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journal_title:Domestic animal endocrinology
pub_type: 杂志文章
doi:10.1016/j.domaniend.2012.01.006
更新日期:2012-07-01 00:00:00
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journal_title:Domestic animal endocrinology
pub_type: 杂志文章,评审
doi:10.1016/j.domaniend.2015.08.003
更新日期:2016-01-01 00:00:00
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journal_title:Domestic animal endocrinology
pub_type: 杂志文章
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更新日期:2000-10-01 00:00:00