Abstract:
:Muscle-targeted gene therapy using insulin genes has the potential to provide an inexpensive, low maintenance alternative or adjunctive treatment method for canine diabetes mellitus. A canine skeletal muscle cell line was established through primary culture, as well as through transdifferentiation of canine fibroblasts after infection with a myo-differentiation gene containing adenovirus vector. A novel mutant furin-cleavable canine preproinsulin gene insert (cppI4) was designed and created through de novo gene synthesis. Various cell lines, including the generated canine muscle cell line, were transfected with nonviral plasmids containing cppI4. Insulin and desmin immunostaining were used to prove insulin production by muscle cells and specific canine insulin ELISA to prove mature insulin secretion into the medium. The canine myoblast cultures proved positive on desmin immunostaining. All cells tolerated transfection with cppI4-containing plasmid, and double immunostaining for insulin and desmin proved present in the canine cells. Canine insulin ELISA assessment of medium of cppI4-transfected murine myoblasts and canine myoblast and fibroblast mixture proved presence of mature fully processed canine insulin, 24 and 48 h after transfection. The present study provides proof of principle that canine muscle cells can be induced to produce and secrete canine insulin on transfection with nonviral plasmid DNA containing a novel mutant canine preproinsulin gene that produces furin-cleavable canine preproinsulin. This technology could be developed to provide an alternative canine diabetes mellitus treatment option or to provide a constant source for background insulin, as well as C-peptide, alongside current treatment options.
journal_name
Domest Anim Endocrinoljournal_title
Domestic animal endocrinologyauthors
Niessen SJ,Fernandez-Fuente M,Mahmoud A,Campbell SC,Aldibbiat A,Huggins C,Brown AE,Holder A,Piercy RJ,Catchpole B,Shaw JA,Church DBdoi
10.1016/j.domaniend.2012.01.006subject
Has Abstractpub_date
2012-07-01 00:00:00pages
16-25issue
1eissn
0739-7240issn
1879-0054pii
S0739-7240(12)00008-2journal_volume
43pub_type
杂志文章abstract::Three experiments were performed to study effects of decreased concentrations of estradiol-17 beta (E2) on lifespan and function of ensuing ovine corpora lutea (CL). In experiment 1, 52 follicles were collected from 10 ewes and placed into individual culture with 0 or .01 microCi 3H-androstenedione (10 ng; 3H-A) and 0...
journal_title:Domestic animal endocrinology
pub_type: 杂志文章
doi:10.1016/0739-7240(92)90019-t
更新日期:1992-10-01 00:00:00
abstract::The ability of the neurohormone melatonin to ameliorate cryopreservation-induced damage to spermatozoa has been demonstrated in several domestic species. However, it is unclear how these protective effects are conferred, with improvements in sperm quality ambiguously attributed to the general antioxidant activity of m...
journal_title:Domestic animal endocrinology
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abstract::The separate and combined effects of Synovex-S (SYN) ear implants and thyroxine (T4)-5'-monodeiodinase inhibition (Trial 1) and T3 injection to create a mild elevation in circulating T3 concentrations (Trial 2) on BW gain and composition of gain were studied. Trial 1 used 24, 285-kg Angus steers in two experimental ph...
journal_title:Domestic animal endocrinology
pub_type: 临床试验,杂志文章
doi:10.1016/0739-7240(92)90030-2
更新日期:1992-07-01 00:00:00
abstract::Adrenomedullin (AM) has been characterized as an endogenous tissue survival factor and modulator of many inflammatory processes. Because of the increased susceptibility of the mammary gland to infection during the time surrounding parturition in the cow, we investigated how milk and tissue content of AM and its bindin...
journal_title:Domestic animal endocrinology
pub_type: 杂志文章
doi:10.1016/j.domaniend.2006.02.004
更新日期:2007-02-01 00:00:00
abstract::The objectives of the present study were to evaluate and compare the accuracy of transabdominal ultrasonography and pregnancy-associated glycoprotein (PAG) assay in the diagnosis of twin pregnancies in ewes and to evaluate the utility of the PAG assay for predicting fetal gender in singleton pregnancies. The animals i...
journal_title:Domestic animal endocrinology
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doi:10.1016/j.domaniend.2019.106399
更新日期:2020-07-01 00:00:00
abstract::Highly prolific sows often experience peripartum hypophagia, resulting in decreased production rate. Leptin, ghrelin, and resistin are known as feed intake-regulating hormones in many species, but it is yet unknown how feeding strategy and body condition will affect these hormones around parturition in sows. In the pr...
journal_title:Domestic animal endocrinology
pub_type: 杂志文章
doi:10.1016/j.domaniend.2013.04.003
更新日期:2013-07-01 00:00:00
abstract::Pituitary-dependent hyperadrenocorticism (PDH) in dogs is caused by a pituitary corticotroph adenoma. Although PDH is a common disorder in dogs, little is known about the underlying pathogenesis. In the pituitary glands of humans and mice, the pro-opiomelanocortin (POMC)-expressing cell lineages, the corticotrophs and...
journal_title:Domestic animal endocrinology
pub_type: 杂志文章
doi:10.1016/j.domaniend.2007.03.002
更新日期:2008-04-01 00:00:00
abstract::To evaluate the estrogenic potential of secoisolariciresinol diglycoside (SDG) found in linseed meal (LSM) on visceral organ mass, IGF-I, and thyroid hormone (T(3) and T(4)) concentrations, 48 multiparous, ovariectomized ewes (54.6 +/- 1.1 kg) were used in a 3 x 4 factorial arrangement. Main effects were length of LSM...
journal_title:Domestic animal endocrinology
pub_type: 杂志文章
doi:10.1016/j.domaniend.2009.05.001
更新日期:2009-10-01 00:00:00
abstract::A large amount of research, primarily in mammals, has defined to a great extent the pleiotropic effects of the IGF system on growth, development, and intermediary metabolism. Similar elucidations in poultry were hindered to some extent by the absence of native peptides (IGF-I and IGF-II) until their purification, foll...
journal_title:Domestic animal endocrinology
pub_type: 杂志文章,评审
doi:10.1016/s0739-7240(97)00019-2
更新日期:1997-07-01 00:00:00
abstract::Dogs do not appear to progress from obesity-induced insulin resistance to type 2 diabetes mellitus. Both postprandial hyperglycemia and postprandial hypertriglyceridemia have been proposed to cause or maintain beta cell failure and progression to type 2 diabetes mellitus in other species. Postprandial glucose, triglyc...
journal_title:Domestic animal endocrinology
pub_type: 杂志文章
doi:10.1016/j.domaniend.2011.10.002
更新日期:2012-02-01 00:00:00
abstract::Increased secretion of prostaglandin F(2)α (PGF(2)α) within the uterus because of uterine inflammation can cause luteolysis and result in early embryonic loss. Supplementation with polyunsaturated fatty acids (PUFAs) has been shown to influence PG production in many species, although the effects on the mare remain unk...
journal_title:Domestic animal endocrinology
pub_type: 杂志文章
doi:10.1016/j.domaniend.2012.09.002
更新日期:2013-01-01 00:00:00
abstract::We aimed to elucidate the effects of PGE2 and PGF2α on the in vitro maturation (IVM) of bovine oocytes. First, cumulus-oocyte complexes were matured in the media supplemented with or without PGE2, PGF2α, or PGE2 plus PGF2α for the final 24, 12, or 6 h of culture. Then, the cumulus-oocyte complexes were matured in the ...
journal_title:Domestic animal endocrinology
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doi:10.1016/j.domaniend.2020.106447
更新日期:2020-07-01 00:00:00
abstract::Eighteen crossbred boars, three siblings from each of six litters, were distributed randomly among three groups after weaning. The 'Natural long-day' group was housed in a standard room with windows, whereas the 'Artificial long-day' and 'Artificial short-day' groups were housed in light-sealed rooms and under an arti...
journal_title:Domestic animal endocrinology
pub_type: 杂志文章
doi:10.1016/s0739-7240(00)00083-7
更新日期:2000-11-01 00:00:00
abstract::The objective of this study was to illuminate the changes in serum NEFA concentrations during a combined glucose-insulin test (CGIT) and basal serum triacylglycerides (TGs) with increasing BW in Shetland ponies and warmblood horses. Therefore, basal blood samples were taken during fasting and a CGIT was performed in 1...
journal_title:Domestic animal endocrinology
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doi:10.1016/j.domaniend.2019.01.003
更新日期:2019-07-01 00:00:00
abstract::Thyroxine (T4) and triiodothyronine (T3) concentrations in pregnant and nonpregnant bitches were measured. The allantoic and amniotic fluid samples were collected separately in the third week of pregnancy, and fetal blood samples were collected in the fourth week of pregnancy. There was no difference between T4 result...
journal_title:Domestic animal endocrinology
pub_type: 杂志文章
doi:10.1016/j.domaniend.2016.07.004
更新日期:2017-01-01 00:00:00
abstract::Four experiments were conducted to evaluate the accuracy and reliability of noninvasive evaluation of cortisol in saliva of dogs. In experiment 1, we measured the cortisol concentration in the filter paper on which 250-μL cortisol solutions had been quantitatively pipetted and in filter papers dipped in cortisol solut...
journal_title:Domestic animal endocrinology
pub_type: 杂志文章
doi:10.1016/j.domaniend.2013.09.008
更新日期:2014-01-01 00:00:00
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journal_title:Domestic animal endocrinology
pub_type: 杂志文章
doi:10.1016/s0739-7240(96)00115-4
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abstract::The objective of transgenic livestock improvement projects is to develop and bring to market superior breeding stock, as well as germplasm for the artificial insemination and embryo transfer industries. Livestock animal biotechnology programs hold the promise of achieving, in a single generation, improvements in comme...
journal_title:Domestic animal endocrinology
pub_type: 杂志文章,评审
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journal_title:Domestic animal endocrinology
pub_type: 杂志文章,评审
doi:10.1016/j.domaniend.2005.02.005
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journal_title:Domestic animal endocrinology
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journal_title:Domestic animal endocrinology
pub_type: 杂志文章,评审
doi:10.1016/j.domaniend.2015.08.003
更新日期:2016-01-01 00:00:00
abstract::The present study was conducted to evaluate changes in follicular fluid (FF) insulin-like growth factor binding protein (IGFBP) proteolytic activity and levels of steroids and IGFBP during follicular development in cattle. Estrous cycles of cows were synchronized with two injections of prostaglandin F2alpha (PGF) 11 d...
journal_title:Domestic animal endocrinology
pub_type: 杂志文章
doi:10.1016/s0739-7240(01)00103-5
更新日期:2001-07-01 00:00:00
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journal_title:Domestic animal endocrinology
pub_type: 杂志文章
doi:10.1016/s0739-7240(01)00107-2
更新日期:2001-08-01 00:00:00
abstract::Hypocalcemia is a common finding in critically ill equine patients. Parathyroid hormone (PTH) helps to maintain calcium homeostasis in hypocalcemic patients by promoting renal calcium reabsorption and bone resorption. Increased serum PTH concentrations have been reported in critically ill people and animals, including...
journal_title:Domestic animal endocrinology
pub_type: 杂志文章
doi:10.1016/j.domaniend.2020.106470
更新日期:2020-07-01 00:00:00
abstract::This study was aimed at testing the hypothesis that insulin-like growth factor binding protein (IGFBP)-3 can modulate hormone-dependent differentiation of granulosa cells in vitro. Granulosa cells from small (1 to 5 mm) follicles were collected from cattle, cultured for 2 d in medium containing 10% fetal calf serum, w...
journal_title:Domestic animal endocrinology
pub_type: 杂志文章
doi:10.1016/s0739-7240(98)00049-6
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abstract::beta-Casein (CSN2) is a major milk protein in most mammals. The CSN2 gene is generally induced by lactogenic hormones bound to its promoter. The expression of this gene can be enhanced by signal transducers and activators of transcription (STAT) and glucocorticoid receptor (GR). Here, we analyzed the promoter and intr...
journal_title:Domestic animal endocrinology
pub_type: 杂志文章
doi:10.1016/j.domaniend.2008.04.001
更新日期:2008-10-01 00:00:00
abstract::Relatively little is known about the physiological roles of microRNAs (miRNAs) during follicular development. Previous evidence from in vitro studies suggests specific roles for a subset of miRNAs, including miR-21, miR-23a, miR-145, miR-503, miR-224, miR-383, miR-378, miR-132, and miR-212, in regulating ovarian folli...
journal_title:Domestic animal endocrinology
pub_type: 杂志文章
doi:10.1016/j.domaniend.2013.06.006
更新日期:2013-10-01 00:00:00
abstract::Tyrosine aminotransferase (TAT) catalyzes the transamination of tyrosine to p-hydroxyphenylpyruvate. Accumulation of tyrosine in the body due to a genetic mutation in the TAT gene causes tyrosomia type II in humans. The TAT gene is regarded as a model for studying steroid-inducible factors regulating a variety of biol...
journal_title:Domestic animal endocrinology
pub_type: 杂志文章
doi:10.1016/j.domaniend.2016.05.001
更新日期:2016-10-01 00:00:00
abstract::This is a test-report of ghrelin levels in plasma and proventriculus, the glandular portion of the avian stomach, by using a specific radioimmunoassay for acylated ghrelin, as well as the expression of the ghrelin gene in the proventriculus after a 12-h fasting period followed by a 6-h feeding period with 6-day-old la...
journal_title:Domestic animal endocrinology
pub_type: 杂志文章
doi:10.1016/j.domaniend.2006.03.006
更新日期:2007-05-01 00:00:00
abstract::The identification of hormones and regulatory factors in colostrum and milk has led to intensive investigations on their roles in the development and maintenance of the mammary and neonatal tissues. Insulin-like growth factors (IGFs) and IGF binding proteins (IGFBPs) in transgenic mice influence mammary biology gland ...
journal_title:Domestic animal endocrinology
pub_type: 杂志文章,评审
doi:10.1016/s0739-7240(02)00149-2
更新日期:2002-07-01 00:00:00