Differential miRNA expression between equine ovulatory and anovulatory follicles.


:Relatively little is known about the physiological roles of microRNAs (miRNAs) during follicular development. Previous evidence from in vitro studies suggests specific roles for a subset of miRNAs, including miR-21, miR-23a, miR-145, miR-503, miR-224, miR-383, miR-378, miR-132, and miR-212, in regulating ovarian follicle development. The objective of this study was to gain insight on the involvement of these miRNAs during follicle maturation. Follicular fluid was aspirated from dominant follicles (>32 mm) during the ovulatory season (July to October) and the anovulatory season (January to March) in each of 5 mares, and the levels of steroids, IGF1, and miRNAs were analyzed by immunoassays and quantitative PCR. Levels of progesterone, testosterone, and IGF1 were lower (P ≤ 0.05) in anovulatory than in ovulatory follicles. Relative to ovulatory follicles, anovulatory follicles had higher (P < 0.05) mean levels of miR-21, miR-23b, miR-378, and miR-202 and tended to have higher (P = 0.06) levels of miR-145. Levels of miR-224 and miR-383 could not be detected in follicular fluid. These novel results indicate a physiological association between increases in follicular miRNA levels and seasonal anovulation in mares; further studies should elucidate the precise involvement of miR-21, miR-23b, miR-145, miR-378, and miR-202 in follicle maturation in the mare.


Domest Anim Endocrinol


Donadeu FX,Schauer SN




Has Abstract


2013-10-01 00:00:00














  • Effect of hyperlipidemia on 11β-hydroxysteroid-dehydrogenase, glucocorticoid receptor, and leptin expression in insulin-sensitive tissues of cats.

    abstract::Glucocorticoid (GC) action depends on GC plasma concentration, cellular GC receptor expression, and the pre-receptor hormone metabolism catalyzed by 11β-hydroxysteroid dehydrogenase (11β-HSD). 11β-Hydroxysteroid dehydrogenase exists in 2 isoforms; 11β-HSD1 converts inactive cortisone to cortisol, and 11β-HSD2 converts...

    journal_title:Domestic animal endocrinology

    pub_type: 杂志文章


    authors: Sieber-Ruckstuhl NS,Zini E,Osto M,Franchini M,Boretti FS,Meli ML,Sigrist B,Lutz TA,Reusch CE

    更新日期:2010-11-01 00:00:00

  • The neuroendocrinology of reproductive behavior in Japanese quail.

    abstract::Sex steroid hormones such as testosterone have widespread effects on brain physiology and function but one of their best characterized effects arguably involves the activation of male sexual behavior. During the past 20 years we have investigated the testosterone control of male sexual behavior in an avian species, th...

    journal_title:Domestic animal endocrinology

    pub_type: 杂志文章,评审


    authors: Balthazart J,Baillien M,Charlier TD,Cornil CA,Ball GF

    更新日期:2003-07-01 00:00:00

  • Effects of body weight gain on insulin and lipid metabolism in equines.

    abstract::The objective of this study was to illuminate the changes in serum NEFA concentrations during a combined glucose-insulin test (CGIT) and basal serum triacylglycerides (TGs) with increasing BW in Shetland ponies and warmblood horses. Therefore, basal blood samples were taken during fasting and a CGIT was performed in 1...

    journal_title:Domestic animal endocrinology

    pub_type: 杂志文章


    authors: Blaue D,Schedlbauer C,Starzonek J,Gittel C,Brehm W,Einspanier A,Vervuert I

    更新日期:2019-07-01 00:00:00

  • Intergenerational impact of maternal overnutrition and obesity throughout pregnancy in sheep on metabolic syndrome in grandsons and granddaughters.

    abstract::We previously reported that maternal overnutrition and obesity (MO) throughout pregnancy and lactation in sheep (MOF0) decreases term fetal pancreatic β-cell numbers and increases perirenal adiposity producing hyperphagia, increased adiposity and insulin resistance in adult female offspring (MOF1) fed ad libitum. Preg...

    journal_title:Domestic animal endocrinology

    pub_type: 杂志文章


    authors: Pankey CL,Walton MW,Odhiambo JF,Smith AM,Ghnenis AB,Nathanielsz PW,Ford SP

    更新日期:2017-07-01 00:00:00

  • Effect of blood handling conditions on progesterone assay results obtained by chemiluminescence in the bitch.

    abstract::Assay of blood progesterone (P4) is commonly practiced to determine the time of ovulation, diagnose luteal insufficiency, and predict time of parturition in bitches. Because of practical constraints, most blood samples cannot be assayed on site immediately after collection. The aim of this work was to evaluate the eff...

    journal_title:Domestic animal endocrinology

    pub_type: 杂志文章


    authors: Tahir MZ,Thoumire S,Raffaelli M,Grimard B,Reynaud K,Chastant-Maillard S

    更新日期:2013-10-01 00:00:00

  • Proteolysis of insulin-like growth factor binding proteins during preovulatory follicular development in cattle.

    abstract::The present study was conducted to evaluate changes in follicular fluid (FF) insulin-like growth factor binding protein (IGFBP) proteolytic activity and levels of steroids and IGFBP during follicular development in cattle. Estrous cycles of cows were synchronized with two injections of prostaglandin F2alpha (PGF) 11 d...

    journal_title:Domestic animal endocrinology

    pub_type: 杂志文章


    authors: Spicer LJ,Chamberlain CS,Morgan GL

    更新日期:2001-07-01 00:00:00

  • cDNA cloning and tissue-specific gene expression of ovine leptin, NPY-Y1 receptor, and NPY-Y2 receptor.

    abstract::The physiological regulation of food intake is a critical factor in both the rate at which an animal grows and its reproductive activity. Recently, progress has been made in elucidating a complex system in which insulin, leptin, and neuropeptide Y function to monitor an animal's energy balance and regulate feed intake...

    journal_title:Domestic animal endocrinology

    pub_type: 杂志文章


    authors: Dyer CJ,Simmons JM,Matteri RL,Keisler DH

    更新日期:1997-09-01 00:00:00

  • Gonadal regulation of LH secretion in prepubertal bull calves.

    abstract::In the present study we examined the influence of castration and exogenous estradiol on pulsatile LH release during the transition from infancy to the prepubertal period of development. Bull calves were assigned to treatments (N = 5 treatment) at 6 weeks of age. Treatments consisted of intact controls, castrates and c...

    journal_title:Domestic animal endocrinology

    pub_type: 杂志文章


    authors: Wise ME,Rodriguez RE,Kelly CM

    更新日期:1987-07-01 00:00:00

  • Role of luteal biosynthesis of prostaglandin F2α on function and structure of the corpus luteum during luteolysis in heifers.

    abstract::The role of endogenous prostaglandin F2α (PGF) in the induction of luteolysis by exogenous PGF was studied by simultaneous inhibition of endogenous PGF with flunixin meglumine (FM). Groups were controls (n = 8), PGF treated (n = 8), and FM + PGF treated (n = 9). Treatments were given 10 d postovulation at hours 0, 8, ...

    journal_title:Domestic animal endocrinology

    pub_type: 杂志文章


    authors: Pinaffi FLV,Araujo ER,Ginther OJ

    更新日期:2018-04-01 00:00:00

  • Bursal antisteroidogenic peptide alters the activity of steroidogenic enzymes in chicken granulosa cells.

    abstract::We have previously reported that a peptide from chicken bursa of Fabricius, bursal antisteroidogenic peptide (BASP), inhibits luteinizing hormone-stimulated progesterone biosynthesis by chicken ovarian granulosa cells. The objective of this study was to determine the site(s) of BASP inhibition within the steroidogenic...

    journal_title:Domestic animal endocrinology

    pub_type: 杂志文章


    authors: Dean CE,Byrd JA,Hargis BM

    更新日期:1995-01-01 00:00:00

  • Interactions between environmental temperature and porcine growth hormone (pGH) treatment in neonatal pigs.

    abstract::Age-dependent interactions between environmental temperature and porcine growth hormone (pGH) treatment on the function of the somatotrophic axis were evaluated in the neonatal pig. At 3 d of age, 40 Landrace x Yorkshire x Duroc piglets received intraperitoneal implants containing either recombinant pGH (0.5 mg/d; n =...

    journal_title:Domestic animal endocrinology

    pub_type: 杂志文章


    authors: Carroll JA,Buonomo FC,Becker BA,Matteri RL

    更新日期:1999-02-01 00:00:00

  • Brain-derived neurotrophic factor in serum as an animal welfare indicator of environmental enrichment in pigs.

    abstract::Environment enrichment is a rising topic for animal welfare but measures to identify effective enrichment interventions are lacking. In humans and rodent species, environmental enrichment increases brain-derived neurotrophic factor (BDNF), the most abundant neurotrophin in the brain. Higher BDNF concentration is ultim...

    journal_title:Domestic animal endocrinology

    pub_type: 杂志文章


    authors: Rault JL,Lawrence AJ,Ralph CR

    更新日期:2018-10-01 00:00:00

  • Differentiation of canine adipose mesenchymal stem cells into insulin-producing cells: comparison of different culture medium compositions.

    abstract::The aim of this study was to differentiate canine adipose-derived mesenchymal stem cells (ADMSCs) into insulin-producing cells by using culture media with different compositions to determine the most efficient media. Stem cells isolated from the fat tissues close to the bitch uterus were distributed into 6 groups: (1)...

    journal_title:Domestic animal endocrinology

    pub_type: 杂志文章


    authors: Camara BOS,Ocarino NM,Bertassoli BM,Malm C,Araújo FR,Reis AMS,Jorge EC,Alves EGL,Serakides R

    更新日期:2021-01-01 00:00:00

  • Effect of nutrient intake during pregnancy on fetal and placental growth and vascular development.

    abstract::Remarkable diversity of size and health of offspring exists after normal pregnancies. When pregnancies are complicated by an extrinsic variable such as inappropriate maternal nutrition, birth weight and health of the neonate are substantially affected. The placenta is the organ through which respiratory gases, nutrien...

    journal_title:Domestic animal endocrinology

    pub_type: 杂志文章,评审


    authors: Redmer DA,Wallace JM,Reynolds LP

    更新日期:2004-10-01 00:00:00

  • Downregulation of LH and FSH receptors after hCG and eCG treatments in the porcine oviduct.

    abstract::The influence of induction of ovulation and superovulation with eCG and hCG on LH and FSH receptor levels in porcine oviducts on day 3 postcoitum was studied. In experiment I, gilts were assigned into cyclic (control; n = 5) and inseminated (n = 5) groups. In experiment II, there were 3 groups of animals: inseminated ...

    journal_title:Domestic animal endocrinology

    pub_type: 杂志文章,随机对照试验


    authors: Małysz-Cymborska I,Andronowska A

    更新日期:2016-10-01 00:00:00

  • Influence of metabolic hormones and nutrition on ovarian follicle development in cattle: practical implications.

    abstract::Nutrition has long been known to have a profound influence on reproductive performance of female cattle, but the underlying mechanism remains poorly understood. Whilst early investigations focused on the modulation of nutrition on hypothalamic-pituitary axis, more recent studies have tested the hypothesis that metabol...

    journal_title:Domestic animal endocrinology

    pub_type: 杂志文章,评审


    authors: Gong JG

    更新日期:2002-07-01 00:00:00

  • Effects of prenatal stress on the fetal calf.

    abstract::Twelve pregnant Brahman cows were randomly assigned to one of two treatment groups: 1) transported in a stock trailer for 24.2 km, unloaded at a second farm and penned for 1 hr, and then returned to the original farm (TRANS, n = 6); or 2) walked through the handling facilities (SHAM, n = 6). Treatments were repeated a...

    journal_title:Domestic animal endocrinology

    pub_type: 杂志文章


    authors: Lay DC Jr,Randel RD,Friend TH,Carroll JA,Welsh TH Jr,Jenkins OC,Neuendorff DA,Bushong DM,Kapp GM

    更新日期:1997-03-01 00:00:00

  • The anorexigenic effect of adrenomedullin in Japanese quail (Coturnix japonica) involves increased proopiomelanocortin and cocaine- and amphetamine-regulated transcript mRNAs in the arcuate nucleus of the hypothalamus.

    abstract::Central administration of adrenomedullin (AM), a 52-amino acid peptide, is associated with anorexigenic effects in some species, including rodents and chickens. However, the associated hypothalamic mechanisms remain unclear and it is unknown if this peptide exerts satiety-inducing effects in other avian species. The o...

    journal_title:Domestic animal endocrinology

    pub_type: 杂志文章


    authors: Wang J,Webster A,Sims W,Gilbert ER,Cline MA

    更新日期:2021-01-01 00:00:00

  • Extrinsic regulation of domestic animal-derived satellite cells.

    abstract::Satellite cells are the postnatal myogenic cells, as they provide myonuclei to support skeletal muscle hypertrophy and are principal cells responsible for myofiber repair and regeneration. Even though research with satellite cells from meat animals is new, considerable data exist to suggest that these cells are regula...

    journal_title:Domestic animal endocrinology

    pub_type: 杂志文章,评审


    authors: Dodson MV,McFarland DC,Grant AL,Doumit ME,Velleman SG

    更新日期:1996-03-01 00:00:00

  • Elevated temperature (heat stress) in vitro reduces androstenedione and estradiol and increases progesterone secretion by follicular cells from bovine dominant follicles.

    abstract::Dairy cattle are susceptible to heat stress-induced reductions in fertility; however, direct effects of hyperthermia on specific reproductive functions are difficult to determine in vivo. The objective of this experiment was to examine the effect of elevated temperature in vitro on follicular steroidogenesis, to gain ...

    journal_title:Domestic animal endocrinology

    pub_type: 杂志文章


    authors: Bridges PJ,Brusie MA,Fortune JE

    更新日期:2005-10-01 00:00:00

  • Dexamethasone treatment at birth enhances neonatal growth in swine.

    abstract::The objective of the present study was to determine if dexamethasone (Dex; a potent synthetic glucocorticoid) treatment at birth would alter postnatal growth in neonatal pigs. Forty crossbred pigs were injected i.m. with either sterile saline (Cont; n = 10 males and 10 females) or Dex (1 mg/kg; n = 10 males and 10 fem...

    journal_title:Domestic animal endocrinology

    pub_type: 杂志文章


    authors: Carroll JA

    更新日期:2001-08-01 00:00:00

  • Sociosexual stimuli and gonadotropin-releasing hormone/luteinizing hormone secretion in sheep and goats.

    abstract::Sociosexual stimuli have a profound effect on the physiology of all species. Sheep and goats provide an ideal model to study the impact of sociosexual stimuli on the hypothalamic-pituitary-gonadal axis because we can use the robust changes in the pulsatile secretion of luteinizing hormone as a bioassay of gonadotropin...

    journal_title:Domestic animal endocrinology

    pub_type: 杂志文章,评审


    authors: Hawken PA,Martin GB

    更新日期:2012-08-01 00:00:00

  • Spontaneously obese dogs exhibit greater postprandial glucose, triglyceride, and insulin concentrations than lean dogs.

    abstract::Dogs do not appear to progress from obesity-induced insulin resistance to type 2 diabetes mellitus. Both postprandial hyperglycemia and postprandial hypertriglyceridemia have been proposed to cause or maintain beta cell failure and progression to type 2 diabetes mellitus in other species. Postprandial glucose, triglyc...

    journal_title:Domestic animal endocrinology

    pub_type: 杂志文章


    authors: Verkest KR,Rand JS,Fleeman LM,Morton JM

    更新日期:2012-02-01 00:00:00

  • Effect of long-term administration of human growth hormone-releasing factor and (or) thyrotropin-releasing factor on hormone concentrations in lactating dairy cows.

    abstract::Fifteen cows (87 +/- 8 d in lactation; 641 +/- 33 kg BW) were randomly assigned to treatment and then subjected for 182 d to daily sc injection (1000 hr), in the cervical area, of saline (control), thyrotropin-releasing factor (TRF: 1 micrograms/kg BW), growth hormone-releasing factor (1-29)NH2 (GRF; 10 micrograms/kg ...

    journal_title:Domestic animal endocrinology

    pub_type: 杂志文章


    authors: Lacasse P,Petitclerc D,Pelletier G,Delorme L,Morisset J,Gaudreau P,Brazeau P

    更新日期:1991-01-01 00:00:00

  • Effects of post-weaning supplementation of immunomodulatory feed ingredient on body weight and cortisol concentrations in program-fed beef heifers.

    abstract::The objective of this study was to determine the effects of an immunomodulatory feed ingredient during post-weaning on growth and cortisol concentrations of beef heifers. Commercial Angus heifers (n = 72) from 2 AI sires were blocked (n = 9) by BW and randomly assigned to one of 2 pens per block. Each pen (4 heifers/p...

    journal_title:Domestic animal endocrinology

    pub_type: 杂志文章


    authors: Danielo J,McCarty KJ,Tipton JE,Ricks RE,Long NM

    更新日期:2020-07-01 00:00:00

  • Effects of the administration of growth hormone-releasing peptide-2 (GHRP-2) orally by gavage and in feed on growth hormone release in swine.

    abstract::The experiments were conducted to determine the effects of the administration of growth hormone-releasing peptide-2 (GHRP-2, also named KP102), both orally by gavage and in feed, on the release of growth hormone (GH) in swine and to investigate whether attenuation of the GH response occurs after short-term treatment w...

    journal_title:Domestic animal endocrinology

    pub_type: 杂志文章


    authors: Phung LT,Sasaki A,Lee HG,Vega RA,Matsunaga N,Hidaka S,Kuwayama H,Hidari H

    更新日期:2001-01-01 00:00:00

  • Mutations in growth hormone gene affect stability of protein structure leading to reduced growth, reproduction, and milk production in crossbred cattle-an insight.

    abstract::The GH has a multifaceted role in growth, reproduction, and milk production. Nonsynonymous mutations identified as L153V were observed from GH1 (L) to GH2 (V) with higher genotypic frequency for GH1 being 0.87. GH2 (V) allele was identified as a rare allele and the population followed Hardy-Weinberg equilibrium. LL ho...

    journal_title:Domestic animal endocrinology

    pub_type: 杂志文章


    authors: Pal A,Pal A,Chakravarty AK

    更新日期:2020-04-01 00:00:00

  • Relationship between serum adiponectin concentration, body condition score, and peripheral tissue insulin response of dairy cows during the dry period.

    abstract::The aim of the present study was to describe the relationship between serum adiponectin concentration and peripheral tissue insulin response in dairy cows with a variable body condition score (BCS) during the dry period. Cows were selected at the beginning of the dry period based on BCS (BCS <3.75, n = 4; BCS >3.75, n...

    journal_title:Domestic animal endocrinology

    pub_type: 杂志文章


    authors: De Koster J,Urh C,Hostens M,Van den Broeck W,Sauerwein H,Opsomer G

    更新日期:2017-04-01 00:00:00

  • Effect of stage of lactation and gestation on milking-induced hormone release in lactating dairy cows.

    abstract::Three experiments were conducted to better understand why milking-induced prolactin (PRL) release decreases as lactation advances. Experiment 1 compared the effects of milking, 2-min manual stimulation of the mammary gland (without milking), or injection of 1 IU of oxytocin (without milking) on hormonal release in ear...

    journal_title:Domestic animal endocrinology

    pub_type: 杂志文章


    authors: Lacasse P,Zhao X,Ollier S

    更新日期:2019-01-01 00:00:00

  • Effects of domperidone and thyrotropin-releasing hormone on secretion of luteinizing hormone and prolactin during the luteal phase and following induction of luteal regression in sheep.

    abstract::Effects of domperidone, a peripheral dopamine receptor antagonist, on secretion of LH and prolactin were studied during the luteal phase and following administration of PGF2 alpha. Since hyperprolactinemia has been reported to inhibit secretion of LH in ewes, effects of thyrotropin-releasing hormone (TRH) also were ex...

    journal_title:Domestic animal endocrinology

    pub_type: 杂志文章


    authors: Deaver DR,Keisler DH,Dailey RA

    更新日期:1987-04-01 00:00:00