Abstract:
:Age-dependent interactions between environmental temperature and porcine growth hormone (pGH) treatment on the function of the somatotrophic axis were evaluated in the neonatal pig. At 3 d of age, 40 Landrace x Yorkshire x Duroc piglets received intraperitoneal implants containing either recombinant pGH (0.5 mg/d; n = 20) or vehicle (control; n = 20). Piglets were maintained at either a low (21 degrees C, 50% relative humidity; n = 20) or high (32 degrees C, 50% relative humidity; n = 20) temperature. At 4 and 6 wk of age, 5 pGH-treated and 5 control piglets from each thermal group were sacrificed for tissue collection. Blood samples were collected at the time of sacrifice and analyzed for serum concentrations of GH, insulin-like growth factor 1 (IGF-1), and IGF-2. Liver RNA was analyzed for mRNAs specific for the GH receptor, IGF-1, IGF-2, and IGF binding protein 3. There was no effect of pGH treatment (P = 0.4) on average daily gain; however, both age (P = 0.002) and temperature (P = 0.001) had an effect on average daily gain such that those animals maintained at a low temperature and those sacrificed at 6 wk had greater average daily gains. Serum concentration of GH was elevated (P = 0.003) by pGH treatment and was lowest in the 6-wk-old group (P = 0.008). Serum concentration of IGF-1 was elevated (P = 0.007) by pGH treatment and increased with age (P = 0.01). Liver GH receptor mRNA was unaffected (P > 0.5) by pGH treatment, but was greater in the 6-wk-old group (P < 0.0001) and in piglets maintained at the high temperature (P = 0.04). IGF-1 mRNA was enhanced by pGH treatment (P = 0.0003) and by exposure to the high temperature (P = 0.04), but did not differ (P > 0.5) between age groups. IGF-2 mRNA was greater (P = 0.0009) in the 4-wk-old group and in piglets maintained at the high temperature (P = 0.007), but was unaffected (P = 0.5) by pGH treatment. IGF binding protein 3 mRNA increased with age (P = 0.0004) and was stimulated by pGH treatment in the 6-wk-old group (P = 0.034). The relatively lower level of GH receptor and IGF mRNAs in conjunction with greater growth in the cold environment suggests that somatotrophic gene expression in the liver is not rate limiting for growth in the neonatal pig.
journal_name
Domest Anim Endocrinoljournal_title
Domestic animal endocrinologyauthors
Carroll JA,Buonomo FC,Becker BA,Matteri RLdoi
10.1016/s0739-7240(99)00003-xsubject
Has Abstractpub_date
1999-02-01 00:00:00pages
103-13issue
2eissn
0739-7240issn
1879-0054pii
S073972409900003Xjournal_volume
16pub_type
杂志文章abstract::Assay of blood progesterone (P4) is commonly practiced to determine the time of ovulation, diagnose luteal insufficiency, and predict time of parturition in bitches. Because of practical constraints, most blood samples cannot be assayed on site immediately after collection. The aim of this work was to evaluate the eff...
journal_title:Domestic animal endocrinology
pub_type: 杂志文章
doi:10.1016/j.domaniend.2013.07.002
更新日期:2013-10-01 00:00:00
abstract::The development of a novel enzyme-linked immunosorbent assay (ELISA) for determining luteinizing hormone (LH) in bovine plasma is described. Anti-bovine LH (bLH) monoclonal antibodies (mAbs) were produced and characterized. One mAb recognizing the bLH β subunit was used for immunoaffinity purification of substantial a...
journal_title:Domestic animal endocrinology
pub_type: 杂志文章
doi:10.1016/j.domaniend.2014.03.004
更新日期:2014-07-01 00:00:00
abstract::In this study, we estimated insulin sensitivity and determined plasma concentrations of total-, low-molecular-weight (LMW), and high-molecular-weight (HMW) adiponectin and leptin in 72 domestic shorthair, neutered, client-owned cats. Glucose tolerance was assessed with an intravenous glucose tolerance test and body fa...
journal_title:Domestic animal endocrinology
pub_type: 杂志文章
doi:10.1016/j.domaniend.2013.11.006
更新日期:2014-04-01 00:00:00
abstract::Thyroid status is compromised in a variety of acute and chronic infections and toxin-mediated disease states. Conversion of thyroxine (T4) into the metabolically active hormone, triiodothyronine (T3), is catalyzed by 5'-deiodinase (5'D). Our objective was to determine the effect of endotoxin (LPS) challenge with and w...
journal_title:Domestic animal endocrinology
pub_type: 杂志文章
doi:10.1016/s0739-7240(99)00069-7
更新日期:2000-01-01 00:00:00
abstract::In Experiment 1, voided urine samples were collected from 12 adult dogs at 0500, 1400, and 2200 hr for 4 days. Cortisol was measured in unextracted urine by radioimmunoassay, creatinine by spectrophotometry, and the cortisol/creatinine ratio (UCCR) was calculated for each sample. There was considerable variation both ...
journal_title:Domestic animal endocrinology
pub_type: 杂志文章
doi:10.1016/0739-7240(90)90013-p
更新日期:1990-10-01 00:00:00
abstract::Ghrelin, the endogenous ligand for growth hormone secretagogue receptor 1a (GHS-R1a), stimulates food intake in mammals centrally and peripherally. In contrast, central injection of ghrelin inhibits feeding in neonatal chicks (Gallus gallus), which is thought to be mediated by the corticotrophin-releasing hormone (CRH...
journal_title:Domestic animal endocrinology
pub_type: 杂志文章
doi:10.1016/j.domaniend.2013.12.002
更新日期:2014-04-01 00:00:00
abstract::The hormone progesterone is essential for proper embryonic development. The objective of this study was to examine the relationship between recipient serum concentrations of progesterone, at the time of embryo transfer and at conceptus recovery, on conceptus development from in vivo- or in vitro-produced embryos. Embr...
journal_title:Domestic animal endocrinology
pub_type: 杂志文章
doi:10.1016/j.domaniend.2015.03.004
更新日期:2015-07-01 00:00:00
abstract::A remote infusion and blood collection device (Dracpac) was used on free-ranging red deer stags to deliver i.v. a range of ACTH1-24 doses and collect blood for cortisol determination. In Experiment 1, conducted in September, saline and 1, 4, and 16 IU ACTH/100 kg bodyweight were infused (n = 5-7 per treatment). In Exp...
journal_title:Domestic animal endocrinology
pub_type: 杂志文章
doi:10.1016/s0739-7240(96)00095-1
更新日期:1997-01-01 00:00:00
abstract::In swine, colostrum production is induced by the drop of progesterone (P4) concentrations which leads to the prepartum peak of prolactin (PRL). PRL regulates mammary cell turnover and stimulates lacteal nutrient synthesis. P4 inhibits PRL secretion and downregulates the PRL receptor in the mammary gland. The aim of th...
journal_title:Domestic animal endocrinology
pub_type: 杂志文章
doi:10.1016/j.domaniend.2015.04.005
更新日期:2015-10-01 00:00:00
abstract::Reduction in fertility is well known to be possibly related to selenium deficiencies, even if target organ for selenium action is, at present, unclear. The present study was aimed to examine whether selenium directly influences granulosa cells. Bovine granulosa cells from different size follicles were used to investig...
journal_title:Domestic animal endocrinology
pub_type: 杂志文章
doi:10.1016/s0739-7240(99)00059-4
更新日期:2000-01-01 00:00:00
abstract::Glucagon-like peptide-1 (GLP-1) is an intestinal hormone that induces glucose-dependent stimulation of insulin secretion while suppressing glucagon secretion. Glucagon-like peptide-1 also increases beta cell mass and satiation while decelerating gastric emptying. Liraglutide is a fatty-acid derivative of GLP-1 with a ...
journal_title:Domestic animal endocrinology
pub_type: 杂志文章
doi:10.1016/j.domaniend.2014.12.001
更新日期:2015-04-01 00:00:00
abstract::The ability of gonadotropin-releasing hormone (GnRH) to synchronize ovulation and new follicular wave emergence before a "superovulatory Day 0" protocol was assessed in Santa Inês ewes. For estrus synchronization, a 60-mg medroxyprogesterone acetate sponge was inserted for 6 d. One day before sponge removal, 37.5-μg d...
journal_title:Domestic animal endocrinology
pub_type: 杂志文章
doi:10.1016/j.domaniend.2015.07.002
更新日期:2016-01-01 00:00:00
abstract::Nutrition has long been known to have a profound influence on reproductive performance of female cattle, but the underlying mechanism remains poorly understood. Whilst early investigations focused on the modulation of nutrition on hypothalamic-pituitary axis, more recent studies have tested the hypothesis that metabol...
journal_title:Domestic animal endocrinology
pub_type: 杂志文章,评审
doi:10.1016/s0739-7240(02)00159-5
更新日期:2002-07-01 00:00:00
abstract::Tyrosine aminotransferase (TAT) catalyzes the transamination of tyrosine to p-hydroxyphenylpyruvate. Accumulation of tyrosine in the body due to a genetic mutation in the TAT gene causes tyrosomia type II in humans. The TAT gene is regarded as a model for studying steroid-inducible factors regulating a variety of biol...
journal_title:Domestic animal endocrinology
pub_type: 杂志文章
doi:10.1016/j.domaniend.2016.05.001
更新日期:2016-10-01 00:00:00
abstract::Environment enrichment is a rising topic for animal welfare but measures to identify effective enrichment interventions are lacking. In humans and rodent species, environmental enrichment increases brain-derived neurotrophic factor (BDNF), the most abundant neurotrophin in the brain. Higher BDNF concentration is ultim...
journal_title:Domestic animal endocrinology
pub_type: 杂志文章
doi:10.1016/j.domaniend.2018.05.007
更新日期:2018-10-01 00:00:00
abstract::Three experiments were performed to study effects of decreased concentrations of estradiol-17 beta (E2) on lifespan and function of ensuing ovine corpora lutea (CL). In experiment 1, 52 follicles were collected from 10 ewes and placed into individual culture with 0 or .01 microCi 3H-androstenedione (10 ng; 3H-A) and 0...
journal_title:Domestic animal endocrinology
pub_type: 杂志文章
doi:10.1016/0739-7240(92)90019-t
更新日期:1992-10-01 00:00:00
abstract::In this study, the expression patterns of inhibins, activins, insulin-like growth factor-I (IGF-I) and steroidogenic enzymes in equine placentae recovered during the latter two-thirds of gestation were examined. Concentrations of inhibin A and inhibin pro-alphaC in endometrial and fetal placental tissue homogenates we...
journal_title:Domestic animal endocrinology
pub_type: 杂志文章
doi:10.1016/j.domaniend.2005.09.005
更新日期:2006-07-01 00:00:00
abstract::To determine if poor maternal nutrition alters growth, body composition, circulating growth factors, and expression of genes involved in the development of muscle and adipose of offspring, 24 Dorset and Shropshire ewes were fed either 100% (control fed), 60% (restricted fed), or 126% (over fed) of National Research Co...
journal_title:Domestic animal endocrinology
pub_type: 杂志文章
doi:10.1016/j.domaniend.2014.05.002
更新日期:2014-10-01 00:00:00
abstract::Satellite cells are the postnatal myogenic cells, as they provide myonuclei to support skeletal muscle hypertrophy and are principal cells responsible for myofiber repair and regeneration. Even though research with satellite cells from meat animals is new, considerable data exist to suggest that these cells are regula...
journal_title:Domestic animal endocrinology
pub_type: 杂志文章,评审
doi:10.1016/0739-7240(95)00062-3
更新日期:1996-03-01 00:00:00
abstract::Muscle-targeted gene therapy using insulin genes has the potential to provide an inexpensive, low maintenance alternative or adjunctive treatment method for canine diabetes mellitus. A canine skeletal muscle cell line was established through primary culture, as well as through transdifferentiation of canine fibroblast...
journal_title:Domestic animal endocrinology
pub_type: 杂志文章
doi:10.1016/j.domaniend.2012.01.006
更新日期:2012-07-01 00:00:00
abstract::It is well established that GnRH can stimulate the release of LH and FSH in mammals. Two GnRHs have been found in the chicken hypothalamus, cGnRH-I and -II. There is controversy as to whether either peptide can stimulate release of FSH in birds. The present studies compared the ability of cGnRH-I and -II to stimulate ...
journal_title:Domestic animal endocrinology
pub_type: 杂志文章
doi:10.1016/j.domaniend.2005.10.002
更新日期:2006-08-01 00:00:00
abstract::Dogs do not appear to progress from obesity-induced insulin resistance to type 2 diabetes mellitus. Both postprandial hyperglycemia and postprandial hypertriglyceridemia have been proposed to cause or maintain beta cell failure and progression to type 2 diabetes mellitus in other species. Postprandial glucose, triglyc...
journal_title:Domestic animal endocrinology
pub_type: 杂志文章
doi:10.1016/j.domaniend.2011.10.002
更新日期:2012-02-01 00:00:00
abstract::Pituitary-dependent hyperadrenocorticism (PDH) in dogs is caused by a pituitary corticotroph adenoma. Although PDH is a common disorder in dogs, little is known about the underlying pathogenesis. In the pituitary glands of humans and mice, the pro-opiomelanocortin (POMC)-expressing cell lineages, the corticotrophs and...
journal_title:Domestic animal endocrinology
pub_type: 杂志文章
doi:10.1016/j.domaniend.2007.03.002
更新日期:2008-04-01 00:00:00
abstract::Highly prolific sows often experience peripartum hypophagia, resulting in decreased production rate. Leptin, ghrelin, and resistin are known as feed intake-regulating hormones in many species, but it is yet unknown how feeding strategy and body condition will affect these hormones around parturition in sows. In the pr...
journal_title:Domestic animal endocrinology
pub_type: 杂志文章
doi:10.1016/j.domaniend.2013.04.003
更新日期:2013-07-01 00:00:00
abstract::The objective of the present study was to evaluate changes in concentrations of free insulin-like growth factor (IGF)-I in follicular fluid (FFL) during follicle development in the mare. Mares (n = 14) were classified as either in the follicular phase (n = 8) or luteal phase (n = 6). Follicles (n = 92) were categorize...
journal_title:Domestic animal endocrinology
pub_type: 杂志文章
doi:10.1016/j.domaniend.2005.03.003
更新日期:2005-11-01 00:00:00
abstract::We studied effects of protein intake at two protein-free energy intake levels on plasma glucose and insulin concentrations, urinary glucose excretion and on liver and intestinal fat content in milk-fed veal calves. Two experiments were performed at body weights (BW) of 80-160 kg (mean 120 kg; Exp. 1) and 160-240 kg (m...
journal_title:Domestic animal endocrinology
pub_type: 杂志文章
doi:10.1016/j.domaniend.2007.10.002
更新日期:2008-08-01 00:00:00
abstract::Twelve pregnant Brahman cows were randomly assigned to one of two treatment groups: 1) transported in a stock trailer for 24.2 km, unloaded at a second farm and penned for 1 hr, and then returned to the original farm (TRANS, n = 6); or 2) walked through the handling facilities (SHAM, n = 6). Treatments were repeated a...
journal_title:Domestic animal endocrinology
pub_type: 杂志文章
doi:10.1016/s0739-7240(96)00115-4
更新日期:1997-03-01 00:00:00
abstract::The influence of induction of ovulation and superovulation with eCG and hCG on LH and FSH receptor levels in porcine oviducts on day 3 postcoitum was studied. In experiment I, gilts were assigned into cyclic (control; n = 5) and inseminated (n = 5) groups. In experiment II, there were 3 groups of animals: inseminated ...
journal_title:Domestic animal endocrinology
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.domaniend.2016.06.003
更新日期:2016-10-01 00:00:00
abstract::Glucocorticoid (GC) action depends on GC plasma concentration, cellular GC receptor expression, and the pre-receptor hormone metabolism catalyzed by 11β-hydroxysteroid dehydrogenase (11β-HSD). 11β-Hydroxysteroid dehydrogenase exists in 2 isoforms; 11β-HSD1 converts inactive cortisone to cortisol, and 11β-HSD2 converts...
journal_title:Domestic animal endocrinology
pub_type: 杂志文章
doi:10.1016/j.domaniend.2010.06.003
更新日期:2010-11-01 00:00:00
abstract::Canine thyroglobulin (cTg) has been isolated and purified. It has similar electrophoretic patterns as Tg from other mammalian species. The main fraction had a MW of 660,000, whereas also fractions of a MW of approximately 1,300,000 (dimer) and 330,000 (subunit) were present. The iodine content was 0.8 to 1.0% (w/w). c...
journal_title:Domestic animal endocrinology
pub_type: 杂志文章
doi:10.1016/0739-7240(91)90020-k
更新日期:1991-10-01 00:00:00