Effect of blood handling conditions on progesterone assay results obtained by chemiluminescence in the bitch.

Abstract:

:Assay of blood progesterone (P4) is commonly practiced to determine the time of ovulation, diagnose luteal insufficiency, and predict time of parturition in bitches. Because of practical constraints, most blood samples cannot be assayed on site immediately after collection. The aim of this work was to evaluate the effects of various sampling and storage conditions on concentrations of P4 as determined by chemiluminescence immunoassay. The blood of 5 Beagle bitches was collected from the jugular vein to study the effect of the type of collection tube (silicone, lithium heparin, EDTA), the storage time of unseparated or separated plasma (2 h to 14 d), and the number of freeze-thaw cycles (1-10) on P4. The effect of each factor was tested within one assay session. None of the factors significantly affected P4. Thus, P4 appears to remain relatively stable in canine blood samples exposed to various processing and storage conditions.

journal_name

Domest Anim Endocrinol

authors

Tahir MZ,Thoumire S,Raffaelli M,Grimard B,Reynaud K,Chastant-Maillard S

doi

10.1016/j.domaniend.2013.07.002

subject

Has Abstract

pub_date

2013-10-01 00:00:00

pages

141-4

issue

3

eissn

0739-7240

issn

1879-0054

pii

S0739-7240(13)00090-8

journal_volume

45

pub_type

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