Abstract:
OBJECTIVE:To explore the expression and function of insulin-like growth factor II (IGFII) mRNA binding protein (IMP3) in the Triple Negative Breast Cancer (TNBC). MATERIALS AND METHODS:According to previously reported gene expression array, we found that IMP3 had significantly higher expression in the CD44+CD24-ESA+ cell cluster, tumor initiating cell or cancer stem cell (CSCs), compared to other tumor cells. Based on the GEO database (GEO accession No. GSE6883), we detected the mRNA levels of IMP 1,2 and 3 by quantitative polymerase chain reaction (q-PCR) in CD44+CD24-ESA+ cell cluster and other breast tumor cell clusters. Besides, we measured IMP3 expression in microsphere of breast cancer, which exerted more significant tumor stem cell properties. The effects of IMP3 on breast cancer cell stem cell properties were studied by RNA interference and overexpression approaches in vitro. Furthermore, we predicted and identified microRNA, which could target and regulate IMP3 from bioinformatics analysis, and verified the interaction by luciferase assays and rescue experiments. RESULTS:Previously reported data showed that IMP3 expression was significantly upregulated in CD44+CD24-ESA+ cell cluster from breast cancer tissues. Besides, we found IMP3 had higher expression in mesenchymal cells rather than epithelial cells, which was also significantly elevated in SUM159 and T49D cell lines cultured as microsphere rather than adherent cells or differentiated cells. CD44+CD24-ESA+ cell cluster proportion was significantly decreased after silencing IMP3 in SUM1315, and its ability to develop into microsphere was significantly inhibited. By re-expressing IMP3 in SUM315, we restored the self-renewal capacity and tumorigenesis potential of SUM315. Through relative predicting website, we found several miRNAs which could regulate IMP3. miR-34a with highest score was chosen for further analysis. Mimicking miR-34a significantly downregulated IMP3 expression and inhibited its ability to develop into microsphere, while overexpressing IMP3 could rescue this process. CONCLUSIONS:IMP3 plays a vital role in maintaining stem cell properties of breast cancer cells, which could be regulated by mir-34a.
journal_name
Eur Rev Med Pharmacol Scijournal_title
European review for medical and pharmacological sciencesauthors
Huang QD,Zheng SR,Cai YJ,Chen DL,Shen YY,Lin CQ,Hu XQ,Wang XH,Shi H,Guo GLdoi
10.26355/eurrev_201805_14965subject
Has Abstractpub_date
2018-05-01 00:00:00pages
2688-2696issue
9eissn
1128-3602issn
2284-0729journal_volume
22pub_type
杂志文章abstract::Pneumatosis intestinalis is an uncommon condition characterized by the presence of gas in the bowel wall. We present the case of a 49-year-old man admitted to our Clinic for his 4 day long haematochezia. Colonoscopy revealed pneumatosis coli as a cause of the lower gastrointestinal bleeding. A wide range of diagnostic...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2013-09-01 00:00:00
abstract::The pathogenesis of diabetes mellitus wounds is complicate, and there lacks effective treatment strategies. Mesenchymal stem cells can promote wound healing. Compared with bone marrow mesenchymal stem cells, umbilical cord mesenchymal stem cells have obvious advantages in biological property. Wnts are potent regulator...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章,评审
doi:
更新日期:2014-01-01 00:00:00
abstract:OBJECTIVE:p38 MAPK are a class of protein kinase that may induce or prevent apoptosis in different circumstance. Emerging researches show that it plays a vital role in tumor progression and therefore understanding its dual role in different stages of lung cancer are important to investigate. Also in this study, we plan...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_201809_15932
更新日期:2018-09-01 00:00:00
abstract:OBJECTIVE:By constructing the severe burns model in rat, we explored the effects of different doses of Ulinastatin (UTI) on protecting myocardium from oxidative stress and inflammatory reaction. MATERIALS AND METHODS:The severe burns model in rat was first constructed. Burned rats were intervened with different doses ...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_201809_15840
更新日期:2018-09-01 00:00:00
abstract:OBJECTIVE:In this study, our purpose was to determine whether plasma BNP level can be useful or not in determining the severity of myocardial injury formed by CO poisoning and to compare plasma BNP level with serum cTnI level. MATERIALS AND METHODS:In the study, 46 female Wistar Albino rats were used. Rats were divide...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2016-01-01 00:00:00
abstract::In this randomized study we compared the efficacy of ondansetron 4 mg with ondansetron 8 mg for the prevention of postoperative nausea and vomiting (PONV) after laparoscopic cholecystectomy with sevoflurane and remifentanil infusion anaesthesia. Sixty patients were randomized to receive ondansetron 8 mg (30 pts) or on...
journal_title:European review for medical and pharmacological sciences
pub_type: 临床试验,杂志文章,随机对照试验
doi:
更新日期:2001-03-01 00:00:00
abstract:OBJECTIVE:Colorectal cancer (CRC) is a leading cause of cancer-related death worldwide. microRNA-198 (miR-198) was reported to be a tumor suppressive miRNA but its role in CRC is largely unknown. Thus, we aimed to investigate the role of miR-198 and its downstream signaling pathway in CRC. PATIENTS AND METHODS:Quantit...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_201902_17106
更新日期:2019-02-01 00:00:00
abstract::In 2015 bladder cancer was the fourth most frequent malignancy and the eighth cause of death for cancer. At diagnosis, about 30% of bladder cancer (BC) patients present a muscle-invasive bladder cancer (MIBC) and 5% a metastatic bladder carcinoma (MBC). For fit MBC patients, combination chemotherapy (CC) is the standa...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_202011_23795
更新日期:2020-11-01 00:00:00
abstract:OBJECTIVE:To investigate whether hypoxia microenvironment induced hepatocellular carcinoma cells SMMC-7721 epithelial-mesenchymal transition (EMT) and to explore the underlying molecular mechanism. MATERIALS AND METHODS:In this study, SMMC-7721 cells were cultured under normoxia and hypoxia conditions, respectively. R...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2017-07-01 00:00:00
abstract:INTRODUCTION:To explore the effect of rosiglitazone on myocardial injury in septic rats through the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) pathway. MATERIALS AND METHODS:A total of 60 healthy adult female Sprague-Dawley (SD) rats were randomly divided into 4 groups, namely, group A (sep...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_202001_19945
更新日期:2020-01-01 00:00:00
abstract:OBJECTIVE:Currently, detection of SARS-CoV-2 RNA is standard in the diagnosis of COVID-19 (2019-nCoV). However, reliable and rapid serological diagnostic methods to screen SARS-CoV-2 infected patients, including those who do not have overt symptoms, are urgently needed. Most studies have described serological tests bas...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章,meta分析,评审
doi:10.26355/eurrev_202010_23243
更新日期:2020-10-01 00:00:00
abstract:OBJECTIVE:To explore the regulatory effect of magnesium sulfate combined with nifedipine and labetalol on disease-related molecules in serum and placenta in the treatment of preeclampsia. PATIENTS AND METHODS:Altogether 100 patients with preeclampsia admitted to the Children & Women's Healthcare of Laiwu City were sel...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_202005_21199
更新日期:2020-05-01 00:00:00
abstract:OBJECTIVE:The present study aimed to explore the role of long non-coding RNA NEAT1 (NEAT1) in mediating non-small cell lung cancer (NSCLC) cell migration and invasion, as well as the underlying regulatory mechanisms. PATIENTS AND METHODS:The NEAT1 expression in NSCLC tissues and cell lines was measured using reverse t...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2017-02-01 00:00:00
abstract:OBJECTIVE:To optimize the management of patients with chronic hepatitis C virus (HCV). MATERIALS AND METHODS:We developed two questionnaires to determine Italian healthcare professionals' opinions on the overall management of HCV chronic liver disease and the use of direct-acting antivirals (DAAs) in the treatment of ...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_201810_16174
更新日期:2018-10-01 00:00:00
abstract::Fourty adult patients with coxarthrosis were treated for 30 days with oral diclofenac sodium at the daily dose of 150 mg: 20 of these were administered one 150 mg prolonged-release capsule per day, the other 20 received one 50 mg enteric-coated tablet every 8 hours. The presence and severity of several symptoms and si...
journal_title:European review for medical and pharmacological sciences
pub_type: 临床试验,杂志文章
doi:
更新日期:1997-01-01 00:00:00
abstract:BACKGROUND AND AIM:Inflammatory cells involved in the pathophysiology of asthma express nicotinic receptor. Therefore 1,1 dimethyl(-4-)phenylpiperazinium (DMPP) in two doses were compared to dexamethasone in asthmatic guinea pigs. MATERIALS AND METHODS:Six groups were included; Normal control and five asthmatic (OVA-s...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2014-01-01 00:00:00
abstract:AIM:Gene expression profiles for intervertebral disc (IVD) cells treated with different osmolarities were compared to identify key genes associated with intervertebral disc diseases. MATERIALS AND METHODS:Microarray data was downloaded from Gene Expression Omnibus (GEO) database and pre-processed using package of R. G...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2013-12-01 00:00:00
abstract:OBJECTIVE:Rheumatic heart disease (RHD) is a serious complication of rheumatic fever (RF). Plasma homocysteine (Hcy) levels are increased in RHD patients. MTHFR catalyzes the irreversible conversion of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate and plays a vital role in Hcy metabolism. We hypothesize th...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2016-01-01 00:00:00
abstract:OBJECTIVE:Vesicoureteral reflux (VUR) affects up to 1% of Caucasian children. Primary VUR is characterized by failure of the ureterovesicular junction to prevent urine from traveling in a retrograde fashion from the bladder to the ureters and the kidneys. Several reports in the literature describe the prevalence of thi...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_201712_13916
更新日期:2017-12-01 00:00:00
abstract:OBJECTIVE:The aim of this study was to identify the role of long non-coding RNA (lncRNA) NBR2 in non-small-cell lung cancer (NSCLC) and its possible molecular mechanisms. PATIENTS AND METHODS:The quantitative Real Time-Polymerase Chain Reaction (qRT-PCR) was used to explore lncRNA NBR2 expression in NSCLC cells and ti...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_201909_19011
更新日期:2019-09-01 00:00:00
abstract:OBJECTIVE:To investigate the influence of micro ribonucleic acid (miR)-21 on rats with rheumatoid arthritis (RA) through the Wnt signaling pathway. MATERIALS AND METHODS:A total of 30 rats were divided into three groups: control group (healthy rats, n=10), model group (rat model of RA, n=10), and MiR group (rat model ...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_201908_18635
更新日期:2019-08-01 00:00:00
abstract:OBJECTIVE:The purpose of this study was to assess the clinical significance of microRNA-4429 (miR-4429) in the development and prognosis of ovarian cancer, and to explore the regulatory role of miR-4429 in migratory potential of ovarian cancer cells. PATIENTS AND METHODS:MiR-4429 levels in paired ovarian cancer specie...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_202009_22809
更新日期:2020-09-01 00:00:00
abstract::In the last 25 years, obesity has reached epidemic levels of prevalence. It has even affected the children such that rates of severe childhood obesity that have almost tripled in numbers. These numbers are alarming because of the known fact that obesity is associated with an increased risk of several comorbidities as ...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章,评审
doi:
更新日期:2015-05-01 00:00:00
abstract:OBJECTIVE:This study explores the possible relation between cervical infections with Ureaplasma urealyticum (UU) and Mycoplasma hominis (MH) and the occurrence of recurrent spontaneous abortion (RSA). PATIENTS AND METHODS:132 patients with RSA (observation group) and 96 normal pregnancy volunteers undergoing planned a...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_201804_14805
更新日期:2018-04-01 00:00:00
abstract:OBJECTIVE:Cyclophosphamide (CTX) can attack tumour cells, but can also damage the other cells and microstructures of an organism at different levels, such as haematopoietic cells, liver cells, peripheral lymphocyte DNA, and genetic materials. Low dose radiation (LDR) can induce general adaptation reaction. In this stud...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2014-01-01 00:00:00
abstract:OBJECTIVE:Hypoxia/reoxygenation (H/R)-induced cardiomyocyte apoptosis plays a critical role in the development of myocardial infarction. Che-1 has been reported as an anti-apoptotic gene in response to various cellular stresses. However, whether Che-1 regulates cardiomyocyte apoptosis in myocardial infarction remains u...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_201802_14395
更新日期:2018-02-01 00:00:00
abstract:OBJECTIVE:This paper aims to evaluate the effects of right ventricular pressure load on left ventricular myocardial mechanics in patients with chronic obstructive pulmonary disease (COPD) by ultrasound speckle tracking imaging. PATIENTS AND METHODS:119 patients with COPD and pulmonary hypertension (PH) were selected f...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_201808_15634
更新日期:2018-08-01 00:00:00
abstract:OBJECTIVE:To investigate the correlations of the computed tomography (CT) signs of non-small cell lung cancer (NSCLC) with pathological features and the expression levels of phosphoprotein 53 (p53) and c-Myc in patients. PATIENTS AND METHODS:87 patients with NSCLC admitted to the Department of Oncology in our hospital...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_201801_14110
更新日期:2018-01-01 00:00:00
abstract:OBJECTIVE:Utilization of nonsteroidal anti-inflammatory drugs (NSAIDs), such as diclofenac, can produce gastrointestinal ulceration. Thus, cyclooxygenase-2-selective inhibitors, such as celecoxib, and protective agents (e.g. rebamipide) have been employed to alleviate harmful NSAID effects. This study sought to explore...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2015-09-01 00:00:00
abstract:OBJECTIVE:The previous work has shown that Berberine and Hesperidin have beneficial effects on cardiovascular diseases. However, the underlying mechanisms remain unknown. This study aimed to investigate the effect of Berberine and Hesperidin on inflammatory cytokine secretion, proliferation, differentiation, and collag...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_201906_18216
更新日期:2019-06-01 00:00:00