A Novel Mechanism for Calmodulin-Dependent Inactivation of Transient Receptor Potential Vanilloid 6.

abstract：:The photobleaching pathway of a short-wavelength cone opsin purified in delipidated form (lambda(max) = 425 nm) is reported. The batho intermediate of the violet cone opsin generated at 45 K has an absorption maximum at 450 nm. The batho intermediate thermally decays to the lumi intermediate (lambda(max) = 435 nm) at ...

journal_title：Biochemistry

authors： Kusnetzow A,Dukkipati A,Babu KR,Singh D,Vought BW,Knox BE,Birge RR

更新日期：2001-07-03 00:00:00

abstract：:Conformational factors that predicate selectivity for valine or isoleucine binding to IlvN leading to the regulation of aceto hydroxy acid synthase I (AHAS I) of Escherichia coli have been determined for the first time from high-resolution (1.9-2.43 Å) crystal structures of IlvN·Val and IlvN·Ile complexes. The valine ...

journal_title：Biochemistry

authors： Bansal A,Karanth NM,Demeler B,Schindelin H,Sarma SP

更新日期：2019-04-16 00:00:00

abstract：:The effect of Mg2+ on the disposition of myosin cross-bridges was studied on myofibrils and synthetic myosin and rod filaments by employing chymotryptic digestion and chemical cross-linking methods. In the presence of low Mg2+ concentrations (0.1 mM), the proteolytic susceptibility at the heavy meromyosin/light meromy...

journal_title：Biochemistry

authors： Reisler E,Liu J,Cheung P

更新日期：1983-10-11 00:00:00

abstract：:Coiled coils (CCs) are among the best-understood protein folds. Nonetheless, there are gaps in our knowledge of CCs. Notably, CCs are likely to be structurally more dynamic than often considered. Here, we explore this in an abundant class of CCs, parallel dimers, focusing on polar asparagine (Asn) residues in the hydr...

journal_title：Biochemistry

authors： Thomas F,Niitsu A,Oregioni A,Bartlett GJ,Woolfson DN

更新日期：2017-12-19 00:00:00

abstract：:Further to our studies on the Escherichia coli 30S ribosomal subunit, the detailed surface topography of both 50S subunits and 70S ribosomes has been investigated by using iodination catalyzed by immobilized lactoperoxidase as the surface probe. In the 50S subunit, only proteins L2, L5, L10, and L11 were iodinated to ...

journal_title：Biochemistry

authors： Maly P,Wower J,Zobawa M,Brimacombe R

更新日期：1983-06-21 00:00:00

abstract：:Time-resolved resonance Raman spectroscopy has been employed to monitor geminate heme-CO rebinding in photolyzed HbCO. The excitation frequency was tuned to enhance the scattering from rebound heme sites 20-500 ns subsequent to CO photolysis. The behavior of vFe-C during ligand rebinding has important ramifications co...

journal_title：Biochemistry

authors： Schneebeck MC,Vigil LE,Friedman JM,Chavez MD,Ondrias MR

更新日期：1993-02-09 00:00:00

abstract：:Kinetic models are presented that allow the Na(+),K(+)-ATPase steady-state turnover number to be estimated at given intra- and extracellular concentrations of Na(+), K(+), and ATP. Based on experimental transient kinetic data, the models utilize either three or four steps of the Albers-Post scheme, that is, E(2) --> E...

journal_title：Biochemistry

authors： Kong BY,Clarke RJ

更新日期：2004-03-02 00:00:00

abstract：:The periplasmic nitrate reductase (NapAB), a member of the DMSO reductase superfamily, catalyzes the first step of the denitrification process in bacteria. In this heterodimer, a di-heme NapB subunit is associated to the catalytic NapA subunit that binds a [4Fe-4S] cluster and a bis(molybdopterin guanine dinucleotide)...

journal_title：Biochemistry

authors： Dementin S,Arnoux P,Frangioni B,Grosse S,Léger C,Burlat B,Guigliarelli B,Sabaty M,Pignol D

更新日期：2007-08-28 00:00:00

abstract：:A mechanism by which the Klenow fragment of DNA polymerase I monitors the geometry of the base pairs may involve hydrogen bonds between the polymerase and the minor groove of the nascent base pair. The involvement of the 3-position of guanine in the template strand was examined by synthesizing oligodeoxynucleotides co...

journal_title：Biochemistry

authors： Spratt TE

更新日期：1997-10-28 00:00:00

abstract：:We have separately expressed the Dictyosteliumdiscoideum myosin II nonhydrolyzer point mutations E459V and E476K [Ruppel, K. M., and Spudich, J. A. (1996) Mol. Biol. Cell 7, 1123-1136] in the soluble myosin head fragment M761-1R [Anson et al. (1996) EMBO J. 15, 6069-6074] and performed transient kinetic analyses to ch...

journal_title：Biochemistry

authors： Friedman AL,Geeves MA,Manstein DJ,Spudich JA

更新日期：1998-07-07 00:00:00

abstract：:Enolase in the presence of Mg2+ catalyzes the elimination of H2O from 2-phosphoglyceric acid (PGA) to form phosphoenolpyruvate (PEP) and the reverse reaction, the hydration of PEP to PGA. The structure of the ternary complex yeast enolase-Mg2(+)-PGA/PEP has been determined by X-ray diffraction and refined by crystallo...

journal_title：Biochemistry

authors： Lebioda L,Stec B

更新日期：1991-03-19 00:00:00

abstract：:Bovine heart ubiquinol-cytochrome c reductase (bc1 complex) was modified with N-(ethoxycarbonyl)-2-ethoxy-1,2-dihydroquinoline (EEDQ), which is a selective reagent for buried carboxyl groups. EEDQ treatment caused a loss of the proton pumping activity of liposome-reconstituted bc1 complex, without effect on the passiv...

journal_title：Biochemistry

authors： Cocco T,Di Paola M,Papa S,Lorusso M

更新日期：1998-02-17 00:00:00

abstract：:Calmodulin is a ubiquitous calcium dependent protein. In the presence of calcium, calmodulin adopts an altered conformation that leads to the generation of downstream cellular calcium signals. Here we describe the introduction of nitroxide EPR probes into calmodulin by means of site-directed spin labeling. These probe...

journal_title：Biochemistry

authors： Shao J,Cieslak J,Gross A

更新日期：2009-01-27 00:00:00

abstract：:The effects of NH3 on the oxygen evolving enzyme have been investigated with EPR and steady-state O2 evolution. The following results were obtained. At low light intensity O2 evolution occurs in all centers even though ammonia is bound. This binding occurs in the S2 state and results in a modification of the multiline...

journal_title：Biochemistry

authors： Boussac A,Rutherford AW,Styring S

更新日期：1990-01-09 00:00:00

abstract：:Hydrolysis of monodisperse short chain phosphatidylcholines, far below their critical micelle concentration, by phospholipase A2 (PLA2) and other interfacial enzymes is characterized. Results show that virtually all the observed hydrolysis by pancreatic and human inflammatory PLA2 occurs on surfaces of the reaction ve...

journal_title：Biochemistry

authors： Yu BZ,Berg OG,Jain MK

更新日期：1999-08-10 00:00:00

abstract：:The interaction of Triton X-100 and other nonionic detergents with a delipidated preparation of the Ca2+ ATPase from sarcoplasmic reticulum has been studied. Binding of radiolabeled Triton X-100 was determined by column chromatography at 6 degrees C, and two classes of binding sites were observed. Below the critical m...

journal_title：Biochemistry

authors： Dean WL,Suárez CP

更新日期：1981-03-31 00:00:00

abstract：:The ability of avian-specific linker histone H5, and the globular domains of H5 (GH5) and H1(0) (GH1(0), to self-associate either free in solution or when bound to DNA was investigated. All three proteins underwent a salt-dependent increase in turbidity that may be indicative of nonspecific interactions. Dithiobis(suc...

journal_title：Biochemistry

authors： Carter GJ,van Holde K

更新日期：1998-09-08 00:00:00

abstract：:The crystal structure and spectroscopic properties of the periplasmic penta-heme cytochrome c nitrite reductase (NrfA) of Escherichia coli are presented. The structure is the first for a member of the NrfA subgroup that utilize a soluble penta-heme cytochrome, NrfB, as a redox partner. Comparison to the structures of ...

journal_title：Biochemistry

authors： Bamford VA,Angove HC,Seward HE,Thomson AJ,Cole JA,Butt JN,Hemmings AM,Richardson DJ

更新日期：2002-03-05 00:00:00

abstract：:Retinitis pigmentosa (RP), a neurodegenerative disorder, can arise from single point mutations in rhodopsin, leading to a cascade of protein instability, misfolding, aggregation, rod cell death, retinal degeneration, and ultimately blindness. Divalent cations, such as zinc and copper, have allosteric effects on misfol...

journal_title：Biochemistry

authors： Gleim S,Stojanovic A,Arehart E,Byington D,Hwa J

更新日期：2009-03-03 00:00:00

abstract：:The UDP-N-acetylglucosamine (UDP-GlcNAc) acyltransferase, encoded by lpxA, catalyzes the first step of lipid A biosynthesis in Gram-negative bacteria, the (R)-3-hydroxyacyl-ACP-dependent acylation of the 3-OH group of UDP-GlcNAc. Recently, we demonstrated that the Arabidopsis thaliana orthologs of six enzymes of the b...

journal_title：Biochemistry

authors： Joo SH,Chung HS,Raetz CR,Garrett TA

更新日期：2012-05-29 00:00:00

abstract：:13C NMR has been used to observe the equilibrium complex of [5,5-2H,5-13C]-5-aminolevulinate [( 5,5-2H,5-13C]ALA) bound to porphobilinogen (PBG) synthase (5-aminolevulinate dehydratase), a 280,000-dalton protein. [5,5-2H,5-13C]ALA (chemical shift 46.9 ppm in D2O) was prepared from [5-13C]ALA through enolization in deu...

journal_title：Biochemistry

authors： Jaffe EK,Markham GD

更新日期：1988-06-14 00:00:00

abstract：:An investigation into the kinetics and regulatory properties of the type-1 phosphofructokinase (PFK) from the extreme thermophile Thermus thermophilus (TtPFK) reveals an enzyme that is inhibited by PEP and activated by ADP by modifying the affinity exhibited for the substrate fructose 6-phosphate (Fru-6-P) in a manner...

journal_title：Biochemistry

authors： McGresham MS,Lovingshimer M,Reinhart GD

更新日期：2014-01-14 00:00:00

abstract：:The kinetics of the interconversions of the subgel and gel phases in dipalmitoylphosphatidylcholine have been studied by using differential dilatometry, differential scanning calorimetry (DSC), and neutral buoyancy centrifugation as a function of incubation temperature and deuteriation of the solvent. As seen by other...

journal_title：Biochemistry

authors： Tristram-Nagle S,Wiener MC,Yang CP,Nagle JF

更新日期：1987-07-14 00:00:00

abstract：:The thylakoid transmembrane DeltapH is the sole energy source driving translocation of precursor proteins by the DeltapH/Tat machinery. Consequently, proton translocation must be coupled to precursor translocation. For the precursor of the 17 kDa protein of the oxygen-evolving complex (pOE17), the protein translocatio...

journal_title：Biochemistry

authors： Musser SM,Theg SM

更新日期：2000-07-18 00:00:00

abstract：:Moesin binds to a large range of proteins through its N terminal FERM (band 4.1, ezrin, radixin, moesin) domain. In full-length moesin isolated from cells, this binding is masked by binding to the C-terminal domain of moesin (C-ERMAD). Activation takes place by phosphorylation of Thr 558 in the C-ERMAD, which releases...

journal_title：Biochemistry

authors： Edwards SD,Keep NH

更新日期：2001-06-19 00:00:00

abstract：:The cyclotides constitute a recently discovered family of plant-derived peptides that have the unusual features of a head-to-tail cyclized backbone and a cystine knot core. These features are thought to contribute to their exceptional stability, as qualitatively observed during experiments aimed at sequencing and char...

journal_title：Biochemistry

authors： Colgrave ML,Craik DJ

更新日期：2004-05-25 00:00:00

abstract：:Synthetic ligands containing up to six residues of nonreducing terminal galactose were prepared. The synthesis involved coupling of carboxyl groups of N-benzyloxy-carbonylaspartic acid or of N-benzyloxycarbonyltyrosyl-gamma-glutamylglutamic acid to the omega-amino group of the aglycon of a glycoside that contained up ...

journal_title：Biochemistry

authors： Lee RT,Lin P,Lee YC

更新日期：1984-08-28 00:00:00

abstract：:The C2 domain is a Ca2+-dependent, membrane-targeting motif originally discovered in protein kinase C and recently identified in numerous eukaryotic signal-transducing proteins, including cytosolic phospholipase A2 (cPLA2) of the vertebrate inflammation pathway. Intracellular Ca2+ signals recruit the C2 domain of cPLA...

journal_title：Biochemistry

authors： Nalefski EA,Slazas MM,Falke JJ

更新日期：1997-10-07 00:00:00

abstract：:Human and mouse metallothionein-3 (MT-3) molecules exhibit the same metal binding stoichiometry with Zn(II), Cd(II), or Cu(I) as MT-1 or MT-2 molecules, suggesting that MT-3 consists of two domains enfolding separate polymetallic clusters. The kinetic reactivities of Zn(II) complexes of MT-3 with the chelator ethylene...

journal_title：Biochemistry

authors： Sewell AK,Jensen LT,Erickson JC,Palmiter RD,Winge DR

更新日期：1995-04-11 00:00:00

abstract：:Elucidation of the molecular mechanism leading to biomembrane fusion is a challenging issue in current biomedical research in view of its involvement in controlling cellular functions and in mediating various important diseases. According to the generally admitted stalk mechanism described for membrane fusion, negativ...

journal_title：Biochemistry

authors： El Kirat K,Dufrêne YF,Lins L,Brasseur R

更新日期：2006-08-01 00:00:00