Abstract:
:Calmodulin is a ubiquitous calcium dependent protein. In the presence of calcium, calmodulin adopts an altered conformation that leads to the generation of downstream cellular calcium signals. Here we describe the introduction of nitroxide EPR probes into calmodulin by means of site-directed spin labeling. These probes sense the calcium-dependent conformational change of calmodulin and, therefore, can serve as calcium indicators. In combination with a light-sensitive calcium chelator, spin-labeled calmodulin can be used to demonstrate calcium release by flash photolysis. These results provide an important step toward describing the molecular dynamics of calcium-induced conformational changes in proteins using EPR spectroscopy.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Shao J,Cieslak J,Gross Adoi
10.1021/bi8014295subject
Has Abstractpub_date
2009-01-27 00:00:00pages
639-44issue
3eissn
0006-2960issn
1520-4995pii
10.1021/bi8014295journal_volume
48pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::The existence of bacterial K(+)/H(+) antiporters that prevent the overaccumulation of potassium in the cytoplasm was predicted by Peter Mitchell almost 50 years ago. The importance of K(+)/H(+) antiport for bacterial physiology is widely recognized, but its molecular mechanisms remain underinvestigated. Here, we demon...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi902173y
更新日期:2010-03-23 00:00:00
abstract::SHAPE-MaP is unique among RNA structure probing strategies in that it both measures flexibility at single-nucleotide resolution and quantifies the uncertainties in these measurements. We report a straightforward analytical framework that incorporates these uncertainties to allow detection of RNA structural differences...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.5b00977
更新日期:2015-11-24 00:00:00
abstract::The effects of metal ion binding on the optical spectroscopic properties and temperature stability of two single tryptophan mutants of chicken skeletal TnC, F78W and F154W, have been examined. The absence of tyrosine and other tryptophan residues allowed the unambiguous assignment of the spectral signal from the intro...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi982048j
更新日期:1999-09-14 00:00:00
abstract::SopE and SopE2 are delivered by the Salmonella type III secretion system into eukaryotic cells to promote cell invasion. SopE and SopE2 are potent guanine nucleotide exchange factors (GEFs) for Rho GTPases Cdc42 and Rac1 and constitute a novel class of Rho GEFs. Although the sequence of SopE-like GEFs is not at all ho...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0490744
更新日期:2004-09-28 00:00:00
abstract::The photoreceptor protein rhodopsin has been reconstituted with a single phospholipid species, dimyristoylphosphatidylcholine, at a range of different lipid/protein ratios, and the exchange rate at the lipid-protein interface has been determined from the electron spin resonance spectra of spin-labeled phosphatidylchol...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00385a045
更新日期:1987-06-02 00:00:00
abstract::Heme propionate-7 in cytochrome c is an ionizable group located in a region of the protein that is inaccessible to bulk solvent. Electrostatic stabilization of this functional group appears to be achieved through interaction of heme propionate-7 with several amino acid residues that occur within hydrogen-bonding dista...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00071a019
更新日期:1993-05-25 00:00:00
abstract::The diflavin reductases exemplified by mammalian cytochrome P450 reductase catalyze NADPH dehydrogenation and electron transfer to an associated monooxygenase. It has recently been proposed that double occupancy of the NADPH dehydrogenation site inhibits the NADPH to FAD hydride transfer step in this series of enzymes...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0360408
更新日期:2004-04-06 00:00:00
abstract::A ribonuclease that hydrolyzes either linear duplex or single-stranded RNA in an exonucleolytic manner has been partially purified from Ehrlich ascites tumor cell nucleoli and is free from other ribonucleases. The enzyme will also degrade the RNA complement of an RNA X DNA duplex; however, no nuclease activity is obse...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00324a022
更新日期:1985-01-29 00:00:00
abstract::Casein kinase II is a widely distributed protein serine/threonine kinase. The holoenzyme appears to be a tetramer, containing two alpha or alpha' subunits (or one of each) and two beta subunits. Complementary DNA clones encoding the subunits of casein kinase II were isolated from a human T-cell lambda gt10 library usi...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00488a034
更新日期:1990-09-11 00:00:00
abstract::Murine class alpha glutathione S-transferase subunit types A2 (mGSTA2-2) and A1 (mGSTA1-1) have high catalytic efficiency for glutathione (GSH) conjugation of the ultimate carcinogenic metabolite of benzo[a]pyrene, (+)-anti-7,8-dihydroxy-9,10-oxy-7,8,9,10-tetrahydrobenzo[a]pyrene, [(+)-anti-BPDE]. Only 10 residues dif...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi026778+
更新日期:2003-02-04 00:00:00
abstract::Apolipoprotein E (apoE) isoforms are key determinants of susceptibility to late-onset Alzheimer's disease (AD). The epsilon 4 and epsilon 2 alleles have been associated with increased and decreased risk for AD, respectively. We have generated and characterized transgenic mice in which the human apoE2 gene is expressed...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi015971l
更新日期:2002-07-30 00:00:00
abstract::Myosin subfragment 1 (S1) was covalently labeled with a fluorescent dye, N-[7-(dimethylamino)-4-methyl-3-coumarinyl]maleimide (DACM), and then digested by trypsin to cleave S1 heavy chain into fragments. The DACM-labeled and trypsin-treated S1 was complexed with F-actin and treated with a zero-length cross-linker, 1-e...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00262a043
更新日期:1982-09-14 00:00:00
abstract::The complete sequence of rabbit antibody light-chain L 120 has been elucidated. The antibody was raised against Micrococcus lysodeikticus bacteria and is specific for the external part of the cell wall. All protein used in this work was obtained from a single 50-mL bleeding. The variable region of L 120 is compared to...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00613a031
更新日期:1978-10-03 00:00:00
abstract::Self-assembly properties of HIV-1 integrase were investigated by time-resolved fluorescence anisotropy using tryptophanyl residues as a probe. From simulation analyses, we show that suitable photon counting leads to an accurate determination of long rotational correlation times in the range of 20-80 ns, permitting the...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi000397j
更新日期:2000-08-08 00:00:00
abstract::The kinetics of activation of Glu-plasminogen (Glu-Pg) and Lys77-Pg by two-chain recombinant tissue plasminogen activator (t-PA) were determined in the presence of isolated protein components of the extracellular matrix (ECM) and compared to activation in the presence of fibrinogen and fibrinogen fragments and in the ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00472a029
更新日期:1990-05-22 00:00:00
abstract::The melanocortin-4 (MC4) receptor is a potential therapeutic target for obesity and cachexia, for which nonpeptide agonists and antagonists are being developed, respectively. The aim of this study was to identify molecular interactions between the MC4 receptor and nonpeptide ligands, and to compare the mechanism of bi...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi051316s
更新日期:2005-11-08 00:00:00
abstract::A substrate analogue, 6-(difluoromethyl)tryptophan, was developed and characterized for mechanistic investigation of tryptophanase. The utility of this derivative was based on its ability to partition between fluoride elimination and carbon-carbon bond scission during tryptophan metabolism. The non-enzymatic hydrolysi...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00221a018
更新日期:1991-02-19 00:00:00
abstract::Apamin is being studied as a model for the folding mechanism of proteins whose structures are stabilized by disulfide bonds. Apamin consists of 18 amino acid residues and forms a stable structure consisting of a C-terminal alpha-helix and two reverse turns. This structure is stabilized by two disulfide bonds connectin...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00133a009
更新日期:1992-05-12 00:00:00
abstract::FHA domains are phosphoThr recognition modules found in diverse signaling proteins, including kinase-associated protein phosphatase (KAPP) from Arabidopsis thaliana. The kinase-interacting FHA domain (KI-FHA) of KAPP targets it to function as a negative regulator of some receptor-like kinase (RLK) signaling pathways i...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi061763n
更新日期:2007-03-13 00:00:00
abstract::Structural changes in the complex formation between transducin and metarhodopsin II, the activated form of photolyzed rhodopsin, in visual transduction processes were analyzed by Fourier transform infrared spectroscopy. The spectrum of the complex was obtained by subtracting the contribution of metarhodopsin I and unc...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi960911e
更新日期:1996-10-15 00:00:00
abstract::The synthesis of 5-(2-oxalylethyl)-NADH, a reduced nicotinamide adenine dinucleotide (NADH) derivate with pyruvate covalently attached to the 5 position of the dihydronicotinamide ring over an additional methylene group has been described previously (Trommer, W.E., Blume, H., and Kapmeyer, H. (1976) Justus Liebigs Ann...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00668a012
更新日期:1976-11-16 00:00:00
abstract::Sucrose density gradient shift assays were used to study the interactions of human glucocorticoid receptors (GR) with small DNA fragments either containing or lacking glucocorticoid response element (GRE) DNA consensus sequences. When crude cytoplasmic extracts containing [3H]triamcinolone acetonide [( 3H]TA) labeled ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00431a003
更新日期:1989-03-07 00:00:00
abstract::Using solution peptide synthesis, we have made three series of N alpha-formylated homooligopeptides, from the dipeptide to the heptapeptide, derived from L-methionine, L-norleucine, and S-methyl-L-cysteine and related to the chemotactic peptide N alpha-formylmethionylleucylphenylalanine. Compounds were prepared to det...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00299a018
更新日期:1984-02-14 00:00:00
abstract::The interaction of the 2'-phosphate-containing nucleotides (NADP+, NADPH, 2'-phosphoadenosine 5'-diphosphoribose, and adenosine 2',5'-bisphosphate) with NADP+ -specific isocitrate dehydrogenase was studied by using 31P NMR spectroscopy. The separate resonances corresponding to free and bound nucleotides, characteristi...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00303a015
更新日期:1984-04-10 00:00:00
abstract::There is considerable evidence that mammalian beta-tubulin is phosphorylated. Specifically, of the seven beta isotypes, the phosphorylated one is beta III, the isotype found almost entirely in neurons. The phosphate is added at a serine and perhaps a tyrosine near the C-terminus. All the evidence to date has been gath...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi951247p
更新日期:1996-03-26 00:00:00
abstract::The kinetics and mechanism of cleavage of DNA by the insulin-mimetic peroxo-vanadate NH4[VO(O2)2(phen)], pV, are described. In the presence of low energy UV radiation or biologically common reducing agents, pV decomposes into the monomer, dimer, and tetramer of vanadate and an uncharacterized compound of V4+ as shown ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9606253
更新日期:1996-09-24 00:00:00
abstract::A subgroup of enzymes in the NAD(P)H:FMN reductase family is comprised of flavin reductases from two-component monooxygenase systems. The diverging structural feature in these FMN reductases is a π-helix centrally located at the tetramer interface that is generated by the insertion of an amino acid in a conserved α4 h...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.8b00544
更新日期:2018-07-31 00:00:00
abstract::Folding models suggest that the highly conserved histidine 217 of the cytochrome b subunit from the cytochrome bc1 complex is close to the quinone reductase (Qi) site. This histidine (bH217) in the cytochrome b polypeptide of the photosynthetic bacterium Rhodobacter capsulatus has been replaced with three other residu...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00169a014
更新日期:1994-01-25 00:00:00
abstract::Manganese peroxidase (MnP) is an extracellular heme enzyme that catalyzes the peroxide-dependent oxidation of Mn(II) to Mn(III). The Mn(III) is released from the enzyme in complex with oxalate. One heme propionate and the side chains of Glu35, Glu39, and Asp179 were identified as Mn(II) ligands in the 2.0 A resolution...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi047318e
更新日期:2005-05-03 00:00:00
abstract::Messenger RNA degradation is an important component of overall gene expression. During the final step of eukaryotic mRNA degradation, exoribonuclease 1 (Xrn1) carries out 5' → 3' processive, hydrolytic degradation of RNA molecules using divalent metal ion catalysis. To initiate studies of the 5' → 3' RNA decay machine...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.9b01035
更新日期:2020-04-21 00:00:00