Abstract：
:The functional requirements of regenerated calcified tissues are that they enable the tissues to bear a variety of imposed stress and consequent contact-induced strain without substantial fracture. Here we demonstrate the effects of glucocorticoid hormones such as dexamethasone and hydrocortisone on the nanomechanical properties of calcified nodules formed by mouse osteoblastic MC3T3-E1 cells in differentiation-inducing medium containing ascorbic acid and β-glycerophosphate. Neither cell proliferation nor calcium deposition, evaluated using alizarin red and von Kossa staining, was affected by dexamethasone. On the other hand, calcified nodules formed in the presence of dexamethasone were significantly harder and stiffer than those formed in their absence. In particular, a series of nanoindentation tests revealed that the calcified nodules formed in the presence of dexamethasone showed enhanced stiffness against dynamic strain as compared to a quasi-static load. Furthermore, Raman spectroscopy revealed that dexamethasone and hydrocortisone increased the apatite/matrix ratio and lowered that of carbonate in the nodules. Our results suggest that glucocorticoids are required for in vitro formation by osteoblasts of more mature calcified nodules containing apatite/phosphate.
journal_name
Acta Biomaterjournal_title
Acta biomaterialiaauthors
Miyamoto S,Miyamoto Y,Shibata Y,Yoshimura K,Izumida E,Suzuki H,Miyazaki T,Maki K,Kamijo Rdoi
10.1016/j.actbio.2014.10.038subject
Has Abstractpub_date
2015-01-01 00:00:00pages
216-226eissn
1742-7061issn
1878-7568pii
S1742-7061(14)00489-9journal_volume
12pub_type
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journal_title：Acta biomaterialia
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pub_type： 杂志文章,评审
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更新日期：2017-03-01 00:00:00
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journal_title：Acta biomaterialia
pub_type： 杂志文章
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journal_title：Acta biomaterialia
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更新日期：2014-05-01 00:00:00
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journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2011.11.026
更新日期：2012-05-01 00:00:00
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journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2012.04.025
更新日期：2012-08-01 00:00:00
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journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2007.03.003
更新日期：2007-09-01 00:00:00
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journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2013.06.017
更新日期：2013-09-01 00:00:00
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journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2019.09.026
更新日期：2019-12-01 00:00:00
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journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2013.09.010
更新日期：2014-01-01 00:00:00
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journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2018.08.006
更新日期：2018-09-15 00:00:00
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journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2016.08.020
更新日期：2016-10-15 00:00:00
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journal_title：Acta biomaterialia
pub_type： 杂志文章
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更新日期：2016-10-01 00:00:00
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pub_type： 杂志文章
doi：10.1016/j.actbio.2007.10.008
更新日期：2008-05-01 00:00:00
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journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2012.01.003
更新日期：2012-04-01 00:00:00
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journal_title：Acta biomaterialia
pub_type： 杂志文章,评审
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更新日期：2013-03-01 00:00:00
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pub_type： 杂志文章
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更新日期：2017-10-15 00:00:00
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journal_title：Acta biomaterialia
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更新日期：2017-01-15 00:00:00
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journal_title：Acta biomaterialia
pub_type： 杂志文章
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更新日期：2013-05-01 00:00:00
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journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2009.12.027
更新日期：2010-06-01 00:00:00
abstract：:Surface coatings delivering BMP are a promising approach to render biomaterials osteoinductive. In contrast to soluble BMPs which can interact with their receptors at the dorsal side of the cell, BMPs presented as an insoluble cue physically bound to a biomimetic matrix, called here matrix-bound (bBMP-2), are presente...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2016.09.014
更新日期：2016-12-01 00:00:00
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journal_title：Acta biomaterialia
pub_type： 杂志文章
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更新日期：2020-10-01 00:00:00
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journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2016.01.030
更新日期：2016-03-01 00:00:00
abstract：:Patterned surfaces direct cell spatial dynamics, yielding cells oriented along the surface geometry, in a process known as contact guidance. The Rho family of GTPases controls the assembly of focal adhesions and cytoskeleton dynamics, but its role in modulating bone-cell alignment on patterned surfaces remains unknown...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2010.11.035
更新日期：2011-04-01 00:00:00
