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Simultaneous detection of intra- and inter-molecular paramagnetic relaxation enhancements in protein complexes.

Abstract：

:Paramagnetic relaxation enhancement (PRE) measurements constitute a powerful approach for detecting both permanent and transient protein-protein interactions. Typical PRE experiments require an intrinsic or engineered paramagnetic site on one of the two interacting partners; while a second, diamagnetic binding partner is labeled with stable isotopes (15N or 13C). Multiple paramagnetic labeled centers or reversed labeling schemes are often necessary to obtain sufficient distance restraints to model protein-protein complexes, making this approach time consuming and expensive. Here, we show a new strategy that combines a modified pulse sequence (1HN-Γ2-CCLS) with an asymmetric labeling scheme to enable the detection of both intra- and inter-molecular PREs simultaneously using only one sample preparation. We applied this strategy to the non-covalent dimer of ubiquitin. Our method confirmed the previously identified binding interface for the transient di-ubiquitin complex, and at the same time, unveiled the internal structural dynamics rearrangements of ubiquitin upon interaction. In addition to reducing the cost of sample preparation and speed up PRE measurements, by detecting the intra-molecular PRE this new strategy will make it possible to measure and calibrate inter-molecular distances more accurately for both symmetric and asymmetric protein-protein complexes.

journal_name

J Biomol NMR

journal_title

Journal of biomolecular NMR

authors

Olivieri C,Subrahmanian MV,Xia Y,Kim J,Porcelli F,Veglia G

doi

10.1007/s10858-018-0165-6

subject

Has Abstract

pub_date

2018-03-01 00:00:00

pages

133-140

issue

3

eissn

0925-2738

issn

1573-5001

pii

10.1007/s10858-018-0165-6

journal_volume

70

pub_type

杂志文章

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