Abstract:
:Relaxation measurements of side-chain 13CH2-groups of uniformly 13C labeled human ubiquitin were performed at 600 MHz and 800 MHz magnetic field strength at 30 degrees C. Dipole-dipole cross-correlated relaxation effects in T1 experiments were suppressed by the combination of radio-frequency pulses and pulsed field gradients during the relaxation delay leading to monoexponential relaxation decays that allow a more accurate extraction of the 13C T1 relaxation times. Heteronuclear [1H]-13C NOEs obtained by using different proton saturation schemes indicate that the influence of cross-correlation is small. The experimental T1 and NOE data were interpreted in a model-free way in terms of a generalized order parameter and an internal correlation time.
journal_name
J Biomol NMRjournal_title
Journal of biomolecular NMRauthors
Jin C,Prompers JJ,Brüschweiler Rdoi
10.1023/a:1023833407515subject
Has Abstractpub_date
2003-07-01 00:00:00pages
241-7issue
3eissn
0925-2738issn
1573-5001pii
5123921journal_volume
26pub_type
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