Abstract:
:Four novel amino acid type-selective triple resonance experiments to identify the backbone amino proton and nitrogen resonances of Arg and Lys and of their sequential neighbors in (13C,15N)-labeled proteins are presented: the R(i+1)-HSQC and R(i,i+1)-HSQC select signals originating from Arg side chains, the K(i+1)-HSQC and K(i,i+1)-HSQC select signals originating from Lys side chains. The selection is based on exploiting the characteristic chemical shifts of a pair of carbon atoms in Arg and Lys side chains using selective 90 degree pulses. The new experiments are recorded as two-dimensional 1H-15N-correlations and their performance is demonstrated with the application to a protein domain of 83 amino acids.
journal_name
J Biomol NMRjournal_title
Journal of biomolecular NMRauthors
Schubert M,Oschkinat H,Schmieder Pdoi
10.1023/a:1011206131623subject
Has Abstractpub_date
2001-08-01 00:00:00pages
379-84issue
4eissn
0925-2738issn
1573-5001journal_volume
20pub_type
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