Abstract:
:NMR relaxometry plays crucial role in studies of protein dynamics. The measurement of longitudinal and transverse relaxation rates of [Formula: see text]N is the main source of information on backbone motions. However, even the most basic approach exploiting a series of [Formula: see text]N HSQC spectra can require several hours of measurement time. Standard non-uniform sampling (NUS), i.e. random under-sampling of indirect time domain, typically cannot reduce this by more than 2-4[Formula: see text] due to relatively low "compressibility" of these spectra. In this paper we propose an extension of NUS to relaxation delays. The two-dimensional space of [Formula: see text]/[Formula: see text] is sampled in a way similar to NUS of [Formula: see text]/[Formula: see text] domain in 3D spectra. The signal is also processed in a way similar to that known from 3D NUS spectra i.e. using one of the most popular compressed sensing algorithms, iterative soft thresholding. The 2D Fourier transform matrix is replaced with mixed inverse Laplace-Fourier transform matrix. The peak positions in resulting 3D spectrum are characterized by two frequency coordinates and relaxation rate and thus no additional fitting of exponential curves is required. The method is tested on three globular proteins, providing satisfactory results in a time corresponding to acquisition of two conventional [Formula: see text]N HSQC spectra.
journal_name
J Biomol NMRjournal_title
Journal of biomolecular NMRauthors
Urbańczyk M,Nowakowski M,Koźmiński W,Kazimierczuk Kdoi
10.1007/s10858-017-0115-8subject
Has Abstractpub_date
2017-06-01 00:00:00pages
155-161issue
2eissn
0925-2738issn
1573-5001pii
10.1007/s10858-017-0115-8journal_volume
68pub_type
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