Abstract:
:We demonstrate a novel methodology to disrupt the symmetry in the NMR spectra of homodimers. A paramagnetic probe is introduced sub-stoichiometrically to create an asymmetric system with the paramagnetic probe residing on only one monomer within the dimer. This creates sufficient magnetic anisotropy for resolution of symmetry-related overlapped resonances and, consequently, detection of pseudocontact shifts and residual dipolar couplings specific to each monomeric component. These pseudocontact shifts can be readily incorporated into existing structure refinement calculations and enable determination of monomer orientation within the dimeric protein. This methodology can be widely used for solution structure determination of symmetric dimers.
journal_name
J Biomol NMRjournal_title
Journal of biomolecular NMRauthors
Gaponenko V,Altieri AS,Li J,Byrd RAdoi
10.1023/a:1020948529076subject
Has Abstractpub_date
2002-10-01 00:00:00pages
143-8issue
2eissn
0925-2738issn
1573-5001journal_volume
24pub_type
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