Abstract:
:Protein-nucleic acid interactions involve intermolecular ion pairs of protein side-chain and DNA or RNA phosphate groups. Using three protein-DNA complexes, we demonstrate that site-specific oxygen-to-sulfur substitution in phosphate groups allows for identification of NMR signals from the protein side-chain NH3 (+) groups forming the intermolecular ion pairs. A characteristic change in their (1)H and (15)N resonances upon this modification (i.e., substitution of phosphate to phosphorodithioate) can represent a signature of an intermolecular ion pair. Hydrogen-bond scalar coupling between protein side-chain (15)N and DNA phosphorodithiaote (31)P nuclei provides direct confirmation of the intermolecular ion pair. The same approach is likely applicable to protein-RNA complexes as well.
journal_name
J Biomol NMRjournal_title
Journal of biomolecular NMRauthors
Anderson KM,Nguyen D,Esadze A,Zandrashvili L,Gorenstein DG,Iwahara Jdoi
10.1007/s10858-015-9909-8subject
Has Abstractpub_date
2015-05-01 00:00:00pages
1-5issue
1eissn
0925-2738issn
1573-5001journal_volume
62pub_type
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