Abstract:
:NMR spectra of ubiquitin in the presence of bicelles at a concentration of 32% w/v have been recorded at 700 MHz under sample spinning conditions at the magic angle (54.7 degrees ) and at an angle of 45.5 degrees . At the magic angle, the 1H-15N HSQC spectrum of ubiquitin in bicelles is virtually indistinguishable from the one recorded on the protein in solution. Spinning the sample at the magic angle creates an isotropic environment with no preferred bicelle orientations, thus allowing the determination of scalar coupling constants. For an angle of rotation of 45.5 degrees , the bicelles orient with their normal perpendicular to the spinning axis leading to the observation of strong residual dipolar couplings and chemical shift variations of the 15N resonances.
journal_name
J Biomol NMRjournal_title
Journal of biomolecular NMRauthors
Lancelot N,Elbayed K,Piotto Mdoi
10.1007/s10858-005-3210-1subject
Has Abstractpub_date
2005-11-01 00:00:00pages
153-61issue
3eissn
0925-2738issn
1573-5001journal_volume
33pub_type
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