Abstract:
:Solid-state NMR spectroscopy (ssNMR) has made significant progress towards the study of membrane proteins in their native cellular membranes. However, reduced spectroscopic sensitivity and high background signal levels can complicate these experiments. Here, we describe a method for ssNMR to specifically label a single protein by repressing endogenous protein expression with rifampicin. Our results demonstrate that treatment of E. coli with rifampicin during induction of recombinant membrane protein expression reduces background signals for different expression levels and improves sensitivity in cellular membrane samples. Further, the method reduces the amount of time and resources needed to produce membrane protein samples, enabling new strategies for studying challenging membrane proteins by ssNMR.
journal_name
J Biomol NMRjournal_title
Journal of biomolecular NMRauthors
Baker LA,Daniëls M,van der Cruijsen EA,Folkers GE,Baldus Mdoi
10.1007/s10858-015-9936-5subject
Has Abstractpub_date
2015-06-01 00:00:00pages
199-208issue
2eissn
0925-2738issn
1573-5001journal_volume
62pub_type
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