Abstract:
:Novel alpha/beta-half-filter elements are proposed for the separation of the high-field and low-field component of 1JHC and 1JHN splittings into different subspectra. The alpha/beta-half-filter elements are of the same duration as the S3CT pulse sequence element and, like this, are less sensitive to cross talk between different subspectra than the original shorter alpha/beta-half-filters. The filter elements are demonstrated with the measurement of 1JHC coupling constants of C alpha H groups in 2D and 3D experiments and the subspectral editing of the four different multiplet components observed in two-dimensional alpha/beta-HSQC-alpha/beta spectra recorded without heteronuclear decoupling in either dimension.
journal_name
J Biomol NMRjournal_title
Journal of biomolecular NMRauthors
Andersson P,Weigelt J,Otting Gdoi
10.1023/a:1008239027287subject
Has Abstractpub_date
1998-10-01 00:00:00pages
435-41issue
3eissn
0925-2738issn
1573-5001journal_volume
12pub_type
杂志文章abstract::Silkworms serve as promising bioreactors for the production of recombinant proteins, including glycoproteins and membrane proteins, for structural and functional protein analyses. However, lack of methodology for stable isotope labeling has been a major deterrent to using this expression system for nuclear magnetic re...
journal_title:Journal of biomolecular NMR
pub_type: 杂志文章
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更新日期:2015-06-01 00:00:00
abstract::Extensive spectral overlap presents a major problem for the NMR study of large RNAs. Here we present NMR techniques for resolution enhancement and spectral simplification of fully 13C labelled RNA. High-resolution 1H-13C correlation spectra are obtained by combining TROSY-type experiments with multiple-band-selective ...
journal_title:Journal of biomolecular NMR
pub_type: 杂志文章
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abstract::Description of protein dynamics is known to be essential in understanding their function. Studies based on a well established [Formula: see text] NMR relaxation methodology have been applied to a large number of systems. However, the low dispersion of [Formula: see text] chemical shifts very often observed within intr...
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更新日期:2017-11-01 00:00:00
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journal_title:Journal of biomolecular NMR
pub_type: 杂志文章
doi:10.1007/s10858-014-9835-1
更新日期:2014-06-01 00:00:00
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journal_title:Journal of biomolecular NMR
pub_type: 杂志文章
doi:10.1007/s10858-006-9000-6
更新日期:2006-05-01 00:00:00
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journal_title:Journal of biomolecular NMR
pub_type: 杂志文章
doi:10.1023/a:1019854626147
更新日期:2002-07-01 00:00:00
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journal_title:Journal of biomolecular NMR
pub_type: 杂志文章
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更新日期:2008-04-01 00:00:00
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journal_title:Journal of biomolecular NMR
pub_type: 杂志文章
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更新日期:2005-08-01 00:00:00
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journal_title:Journal of biomolecular NMR
pub_type: 杂志文章
doi:10.1007/BF00198369
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pub_type: 杂志文章
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更新日期:1993-09-01 00:00:00
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journal_title:Journal of biomolecular NMR
pub_type: 杂志文章
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更新日期:2003-08-01 00:00:00
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journal_title:Journal of biomolecular NMR
pub_type: 杂志文章
doi:10.1007/s10858-015-9909-8
更新日期:2015-05-01 00:00:00
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journal_title:Journal of biomolecular NMR
pub_type: 杂志文章
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更新日期:2012-01-01 00:00:00
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journal_title:Journal of biomolecular NMR
pub_type: 杂志文章
doi:10.1023/a:1008307115641
更新日期:2000-04-01 00:00:00
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journal_title:Journal of biomolecular NMR
pub_type: 杂志文章,评审
doi:10.1007/BF00211749
更新日期:1995-04-01 00:00:00
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journal_title:Journal of biomolecular NMR
pub_type: 杂志文章
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更新日期:2000-11-01 00:00:00
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journal_title:Journal of biomolecular NMR
pub_type: 杂志文章
doi:10.1007/s10858-010-9435-7
更新日期:2010-09-01 00:00:00
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journal_title:Journal of biomolecular NMR
pub_type: 杂志文章
doi:10.1007/s10858-010-9414-z
更新日期:2010-06-01 00:00:00
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journal_title:Journal of biomolecular NMR
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更新日期:2011-04-01 00:00:00
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journal_title:Journal of biomolecular NMR
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更新日期:2013-03-01 00:00:00
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journal_title:Journal of biomolecular NMR
pub_type: 杂志文章
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更新日期:2012-09-01 00:00:00
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journal_title:Journal of biomolecular NMR
pub_type: 杂志文章
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更新日期:1999-06-01 00:00:00
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journal_title:Journal of biomolecular NMR
pub_type: 杂志文章
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更新日期:2001-03-01 00:00:00
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journal_title:Journal of biomolecular NMR
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journal_title:Journal of biomolecular NMR
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journal_title:Journal of biomolecular NMR
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