Abstract:
:The application of metabolic precursors for selective stable isotope labeling of aromatic residues in cell-based protein overexpression has already resulted in numerous NMR probes to study the structural and dynamic characteristics of proteins. With anthranilic acid, we present the structurally simplest precursor for exclusive tryptophan side chain labeling. A synthetic route to 13C, 2H isotopologues allows the installation of isolated 13C-1H spin systems in the indole ring of tryptophan, representing a versatile tool to investigate side chain motion using relaxation-based experiments without the loss of magnetization due to strong 1JCC and weaker 2JCH scalar couplings, as well as dipolar interactions with remote hydrogens. In this article, we want to introduce this novel precursor in the context of hitherto existing techniques of in vivo aromatic residue labeling.
journal_name
J Biomol NMRjournal_title
Journal of biomolecular NMRauthors
Schörghuber J,Geist L,Bisaccia M,Weber F,Konrat R,Lichtenecker RJdoi
10.1007/s10858-017-0129-2subject
Has Abstractpub_date
2017-09-01 00:00:00pages
13-22issue
1eissn
0925-2738issn
1573-5001pii
10.1007/s10858-017-0129-2journal_volume
69pub_type
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