Abstract:
:HET(ex)-SOFAST NMR (Schanda et al. in J Biomol NMR 33:199-211, 2006) has been proposed some years ago as a fast and sensitive method for semi-quantitative measurement of site-specific amide-water hydrogen exchange effects along the backbone of proteins. Here we extend this concept to BEST readout sequences that provide a better resolution at the expense of some loss in sensitivity. We discuss the theoretical background and implementation of the experiment, and demonstrate its performance for an intrinsically disordered protein, 2 well folded globular proteins, and a transiently populated folding intermediate state. We also provide a critical evaluation of the level of accuracy that can be obtained when extracting quantitative exchange rates from HET(ex) NMR measurements.
journal_name
J Biomol NMRjournal_title
Journal of biomolecular NMRauthors
Rennella E,Solyom Z,Brutscher Bdoi
10.1007/s10858-014-9857-8subject
Has Abstractpub_date
2014-11-01 00:00:00pages
99-107issue
2-3eissn
0925-2738issn
1573-5001journal_volume
60pub_type
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