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Identification of solvent-exposed regions of an FK-506 analog, ascomycin, bound to FKBP using a paramagnetic probe.

Abstract：

:The solvent-exposed regions of (U-13C)ascomycin when bound to its putative target protein, FKBP, have been identified based on the different proton longitudinal relaxation rates (R1 = 1/T1) measured in the absence and presence of the paramagnetic relaxation reagent, 4-hydroxy-2,2,6,6-tetramethyl-piperidinyl-1-oxy (HyTEMPO). The proton T1S of bound ascomycin were determined using a pulse sequence (T1-HMQC) which consists of a 180 degree proton pulse and a variable delay (tau) followed by a heteronuclear multiple quantum correlation (HMQC) experiment. The solvent-exposed regions of ascomycin determined by these experiments are compared to NOE data in which ascomycin/FKBP contacts were identified and to the X-ray structure of the FK-506/FKBP complex.

journal_name

J Biomol NMR

journal_title

Journal of biomolecular NMR

authors

Petros AM,Neri P,Fesik SW

doi

10.1007/BF02192797

subject

Has Abstract

pub_date

1992-01-01 00:00:00

pages

11-8

issue

1

eissn

0925-2738

issn

1573-5001

journal_volume

2

pub_type

杂志文章

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