Abstract：
:A pulse sequence is described for recording single-quantum (13)C-methyl relaxation dispersion profiles of (13)C-selectively labeled methyl groups in proteins that offers significant improvements in sensitivity relative to existing approaches where initial magnetization derives from (13)C polarization. Sensitivity gains in the new experiment are achieved by making use of polarization from (1)H spins and (1)H --> (13)C --> (1)H type magnetization transfers. Its utility has been established by applications involving three different protein systems ranging in molecular weight from 8 to 28 kDa, produced using a number of different selective labeling approaches. In all cases exchange parameters from both (13)C-->(1)H and (1)H --> (13)C --> (1)H classes of experiment are in good agreement, with gains in sensitivity of between 1.7 and 4-fold realized using the new scheme.
journal_name
J Biomol NMRjournal_title
Journal of biomolecular NMRauthors
Lundström P,Vallurupalli P,Religa TL,Dahlquist FW,Kay LEdoi
10.1007/s10858-007-9149-7subject
Has Abstractpub_date
2007-05-01 00:00:00pages
79-88issue
1eissn
0925-2738issn
1573-5001journal_volume
38pub_type
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