Narrow carbonyl resonances in proton-diluted proteins facilitate NMR assignments in the solid-state.

Abstract:

:HNCO/HNCACO type correlation experiments are an alternative for assignment of backbone resonances in extensively deuterated proteins in the solid-state, given the fact that line widths on the order of 14-17 Hz are achieved in the carbonyl dimension without the need of high power decoupling. The achieved resolution demonstrates that MAS solid-state NMR on extensively deuterated proteins is able to compete with solution-state NMR spectroscopy if proteins are investigated with correlation times tau(c) that exceed 25 ns.

journal_name

J Biomol NMR

authors

Linser R,Fink U,Reif B

doi

10.1007/s10858-010-9404-1

subject

Has Abstract

pub_date

2010-05-01 00:00:00

pages

1-6

issue

1

eissn

0925-2738

issn

1573-5001

journal_volume

47

pub_type

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