当前位置： SCI文献检索 > BONE期刊下所有文献 > The elementary fusion modalities of osteoclasts.

The elementary fusion modalities of osteoclasts.

Abstract：

:The last step of the osteoclast differentiation process is cell fusion. Most efforts to understand the fusion mechanism have focused on the identification of molecules involved in the fusion process. Surprisingly, the basic fusion modalities, which are well known for fusion of other cell types, are not known for the osteoclast. Here we show that osteoclast fusion partners are characterized by differences in mobility, nuclearity, and differentiation level. Our demonstration was based on time-laps videos of human osteoclast preparations from three donors where 656 fusion events were analyzed. Fusions between a mobile and an immobile partner were most frequent (62%), while fusion between two mobile (26%) or two immobile partners (12%) was less frequent (p<0.001). In general, the immobile fusion partner contained more nuclei than the mobile one (p<0.01). Furthermore, enrichment in nuclei of an osteoclast with three or more nuclei resulted from fusion with a mono-nucleated cell in 67% of the cases (p<0.001), while mono-nucleated cells fused with a multinucleated cell in 61% of the cases (p<0.05). This observation suggested that a more mature osteoclast prefers to fuse with a less mature pre-osteoclast. This hypothesis was supported by a nucleus-tracing approach in a co-culture of more and less differentiated pre-osteoclasts/osteoclasts. Furthermore, we found that osteoclast fusion proceeds through primarily two different types of cell contacts: phagocytic-cup and broad-contact-surfaces (>80% of all fusions). We conclude that osteoclasts most often gain nuclei by addition of one nucleus at a time, and that this nucleus is most often delivered by a moving cell to an immobile cell. These characteristics fit the in vivo observations where mono-nucleated precursors migrating from the bone marrow fuse with more mature osteoclasts sitting on the bone surface. They also fit the fusion modalities of other cell types.

journal_name

Bone

journal_title

Bone

authors

Søe K,Hobolt-Pedersen AS,Delaisse JM

doi

10.1016/j.bone.2014.12.010

subject

Has Abstract

pub_date

2015-04-01 00:00:00

pages

181-9

eissn

8756-3282

issn

1873-2763

pii

S8756-3282(14)00462-1

journal_volume

73

pub_type

杂志文章

相关文献

BONE文献大全
  • Intermittent versus continuous clodronate administration in postmenopausal women with low bone mass.

    abstract：:The aim of the study was to compare the effects on bone mass and turnover of continuous vs. intermittent clodronate administration on 120 postmenopausal women (average age 61 years) with low bone mass (femoral neck bone mineral density [BMD] of at least -1 SD or more, T-score), with another 30 women as a control group...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/s8756-3282(99)00273-2

    authors： Filipponi P,Cristallini S,Policani G,Schifini MF,Casciari C,Garinei P

    更新日期：2000-03-01 00:00:00

  • Animal model for medication-related osteonecrosis of the jaw with precedent metabolic bone disease.

    abstract：:Despite the fact that the medications used to treat abnormal bone conditions often induce osteonecrosis of the jaw (ONJ), previous attempts to establish an animal model for ONJ have shown insufficient consideration for this important prerequisite for the development of the disease. The purpose of this study was to est...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2015.08.012

    authors： Kim JW,Tatad JCI,Landayan MEA,Kim SJ,Kim MR

    更新日期：2015-12-01 00:00:00

  • Epigenetic mechanisms driving lineage commitment in mesenchymal stem cells.

    abstract：:The increasing application of approaches that allow tracing of individual cells over time, together with transcriptomic and epigenomic analyses is changing the way resident stromal stem cells (mesenchymal stem cells) are viewed. Rather than being a defined, homogeneous cell population as described following in vitro e...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2020.115309

    authors： Yianni V,Sharpe PT

    更新日期：2020-05-01 00:00:00

  • Ultrasound stimulates nitric oxide and prostaglandin E2 production by human osteoblasts.

    abstract：:We have previously shown that the therapeutic range of ultrasound heals osteoradionecrotic bone and induces bone formation in vitro. It is well established that nitric oxide (NO) and prostaglandins are crucial early mediators in mechanically induced bone formation. The therapeutic range of ultrasound may act in the sa...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/s8756-3282(02)00789-5

    authors： Reher P,Harris M,Whiteman M,Hai HK,Meghji S

    更新日期：2002-07-01 00:00:00

  • Denosumab in postmenopausal women with osteoporosis and diabetes: Subgroup analysis of FREEDOM and FREEDOM extension.

    abstract：PURPOSE:Diabetes and osteoporosis occur frequently in older adults and are both associated with increased fracture risk. Denosumab treatment reduced new vertebral, nonvertebral, and hip fractures over 3 years, with continued low fracture incidence for up to 10 years in postmenopausal women with osteoporosis. However, i...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2020.115268

    authors： Ferrari S,Eastell R,Napoli N,Schwartz A,Hofbauer LC,Chines A,Wang A,Pannacciulli N,Cummings SR

    更新日期：2020-05-01 00:00:00

  • Age- and gender-related differences in bone mineral status and biochemical markers of bone metabolism in Northern Chinese men and women.

    abstract：:The aim of this study was to provide insight into the bone mineral status and biochemical markers of bone metabolism in a Chinese population from Shenyang, in the north of China, where hip fracture incidence is low. A total of 194 healthy men and women, aged 25-35 years and 65-75 years, were studied. Bone mineral dens...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/s8756-3282(01)00676-7

    authors： Yan L,Prentice A,Zhou B,Zhang H,Wang X,Stirling DM,Laidlaw A,Han Y,Laskey A

    更新日期：2002-02-01 00:00:00

  • Homocysteine enhances apoptosis in human bone marrow stromal cells.

    abstract：INTRODUCTION:High plasma homocysteine (Hcy) levels have been associated with increased risk of fracture. Since Hcy has been shown to induce apoptosis in many cell types, including vascular endothelial cells, we hypothesized that Hcy would have a similar apoptotic effect on osteoblasts, leading to osteoporosis by reduci...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2006.03.004

    authors： Kim DJ,Koh JM,Lee O,Kim NJ,Lee YS,Kim YS,Park JY,Lee KU,Kim GS

    更新日期：2006-09-01 00:00:00

  • Prolonged bisphosphonate release after treatment in women with osteoporosis. Relationship with bone turnover.

    abstract：:Bisphosphonates (BP), especially alendronate and risedronate, are the drugs most commonly used for osteoporosis treatment, being incorporated into the skeleton where they inhibit bone resorption and are thereafter slowly released during bone turnover. However, there are few data on the release of BP in patients who ha...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2011.06.027

    authors： Peris P,Torra M,Olivares V,Reyes R,Monegal A,Martínez-Ferrer A,Guañabens N

    更新日期：2011-10-01 00:00:00

  • Body mass influences cortical bone mass independent of leptin signaling.

    abstract：:Obesity in humans is associated with increased bone mass. Leptin, a hormone produced by fat cells, functions as a sentinel of energy balance, and may mediate the putative positive effects of body mass on bone. We performed studies in male C57Bl/6 wild type (WT) and leptin-deficient ob/ob mice to determine whether body...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2008.10.058

    authors： Iwaniec UT,Dube MG,Boghossian S,Song H,Helferich WG,Turner RT,Kalra SP

    更新日期：2009-03-01 00:00:00

  • Sex-specific compromised bone healing in female rats might be associated with a decrease in mesenchymal stem cell quantity.

    abstract：INTRODUCTION:The clinically known importance of patient sex as a major risk factor for compromised bone healing is poorly reflected in animal models. Consequently, the underlying cellular mechanisms remain elusive. Because mesenchymal stem cells (MSCs) are postulated to regulate tissue regeneration and give rise to ess...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2009.08.005

    authors： Strube P,Mehta M,Baerenwaldt A,Trippens J,Wilson CJ,Ode A,Perka C,Duda GN,Kasper G

    更新日期：2009-12-01 00:00:00

  • Contributions of bone density and geometry to the strength of the human second metatarsal.

    abstract：:We investigated, at the whole bone level, the contribution of bone density and geometry to the fracture load of the second metatarsal, a bone that is prone to stress fracture. Dual-energy X-ray absorptiometry (DXA) was used to determine the areal bone mineral density (BMD), projected area of bone, and bone mineral con...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/s8756-3282(00)00370-7

    authors： Muehleman C,Lidtke R,Berzins A,Becker JH,Shott S,Sumner DR

    更新日期：2000-11-01 00:00:00

  • CSF-1 blockade impairs breast cancer osteoclastogenic potential in co-culture systems.

    abstract：:Metastatic bone disease has a major impact on the morbidity and mortality of breast cancer patients, and studies on bone metastasis biology have led to the development of the most widely used drugs for bone metastases treatment: zoledronate (Zol) and denosumab (Den). The aim of the present study was to assess the effe...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2014.06.017

    authors： Liverani C,Mercatali L,Spadazzi C,La Manna F,De Vita A,Riva N,Calpona S,Ricci M,Bongiovanni A,Gunelli E,Zanoni M,Fabbri F,Zoli W,Amadori D,Ibrahim T

    更新日期：2014-09-01 00:00:00

  • Comparison in localization between cystatin C and cathepsin K in osteoclasts and other cells in mouse tibia epiphysis by immunolight and immunoelectron microscopy.

    abstract：:We compared the distribution of a cysteine proteinase inhibitor, cystatin C, with that of cathepsin K in osteoclasts of the mouse tibia by immunolight and immunoelectron microscopy. Light microscopically, strong immunoreactivity for cystatin C was found extracellularly along the resorption lacuna and intracellularly i...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/s8756-3282(01)00466-5

    authors： Yamaza T,Tsuji Y,Goto T,Kido MA,Nishijima K,Moroi R,Akamine A,Tanaka T

    更新日期：2001-07-01 00:00:00

  • Longitudinal changes in bone mineral density during normal pregnancy.

    abstract：:Pregnancy is a common physiological event that could affect peak bone mass and the risk of developing osteoporosis later in life. There have been few longitudinal studies over a complete reproductive cycle of any size to show whether bone mineral density (BMD) changes. We have measured BMD by dual-energy X-ray absorpt...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/s8756-3282(03)00017-6

    authors： Kaur M,Pearson D,Godber I,Lawson N,Baker P,Hosking D

    更新日期：2003-04-01 00:00:00

  • Reference intervals of biochemical bone turnover markers for Saudi Arabian women: a cross-sectional study.

    abstract：:Biochemical bone turnover markers (BTMs) provide important information on the diagnosis, therapy and monitoring of metabolic bone diseases including osteoporosis. One goal of antiresorptive therapy in women is to decrease biochemical BTMs to the lower half of reference intervals for healthy pre-menopausal counterparts...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2010.07.017

    authors： Ardawi MS,Maimani AA,Bahksh TA,Rouzi AA,Qari MH,Raddadi RM

    更新日期：2010-10-01 00:00:00

  • The longitudinal effects of ovariectomy on the morphometric, densitometric and mechanical properties in the murine tibia: A comparison between two mouse strains.

    abstract：:Oestrogen deficiency-related bone loss in the ovariectomized (OVX) mouse is a common model for osteoporosis. However, a comprehensive in vivo assessment of intervention-related changes in multiple bone properties, and in multiple mouse strains, is required in order to identify an appropriate model for future evaluatio...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2019.06.024

    authors： Roberts BC,Giorgi M,Oliviero S,Wang N,Boudiffa M,Dall'Ara E

    更新日期：2019-10-01 00:00:00

  • Autologous blood coagulum containing rhBMP6 induces new bone formation to promote anterior lumbar interbody fusion (ALIF) and posterolateral lumbar fusion (PLF) of spine in sheep.

    abstract：:In the present study, we evaluated an autologous bone graft substitute (ABGS) composed of recombinant human BMP6 (rhBMP6) dispersed within autologous blood coagulum (ABC) used as a physiological carrier for new bone formation in spine fusion sheep models. The application of ABGS included cervical cage for use in the a...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2020.115448

    authors： Grgurevic L,Erjavec I,Gupta M,Pecin M,Bordukalo-Niksic T,Stokovic N,Vnuk D,Farkas V,Capak H,Milosevic M,Bubic Spoljar J,Peric M,Vuckovic M,Maticic D,Windhager R,Oppermann H,Sampath TK,Vukicevic S

    更新日期：2020-09-01 00:00:00

  • Use of an alpha-smooth muscle actin GFP reporter to identify an osteoprogenitor population.

    abstract：:Identification of a reliable marker of skeletal precursor cells within calcified and soft tissues remains a major challenge for the field. To address this, we used a transgenic model in which osteoblasts can be eliminated by pharmacological treatment. Following osteoblast ablation a dramatic increase in a population o...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2008.04.023

    authors： Kalajzic Z,Li H,Wang LP,Jiang X,Lamothe K,Adams DJ,Aguila HL,Rowe DW,Kalajzic I

    更新日期：2008-09-01 00:00:00

  • A reference standard for the description of osteoporosis.

    abstract：:In 1994, the World Health Organization published diagnostic criteria for osteoporosis. Since then, many new technologies have been developed for the measurement of bone mineral at multiple skeletal sites. The information provided by each assessment will describe the clinical characteristics, fracture risk and epidemio...

    journal_title：Bone

    pub_type： 杂志文章,评审

    doi：10.1016/j.bone.2007.11.001

    authors： Kanis JA,McCloskey EV,Johansson H,Oden A,Melton LJ 3rd,Khaltaev N

    更新日期：2008-03-01 00:00:00

  • Characterization of damage mechanisms associated with reference point indentation in human bone.

    abstract：:Measurement of bone mineral density (BMD) is the clinical gold standard in cases of compromised skeletal integrity, such as with osteoporosis. While BMD is a useful measurement to index skeletal health, it is also limited since it cannot directly assess any mechanical properties. The ability to directly assess mechani...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2015.01.019

    authors： Beutel BG,Kennedy OD

    更新日期：2015-06-01 00:00:00

  • Differences in the microarchitectural features of the lateral collapsed lesion between osteonecrosis and subchondral insufficiency fracture of the femoral head.

    abstract：BACKGROUND:Like osteonecrosis of the femoral head (ONFH), subchondral insufficiency fracture of the femoral head (SIF) causes femoral head collapse. However, little is known about the differences between the two diseases regarding the morphological features of the collapsed lesion. We tested the hypothesis that the mor...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2020.115585

    authors： Kawano K,Motomura G,Ikemura S,Yamaguchi R,Baba S,Xu M,Nakashima Y

    更新日期：2020-12-01 00:00:00

  • The short- and long-term effects of methotrexate on the rat skeleton.

    abstract：:Both clinical and laboratory studies have shown that the chemotherapy drug, Methotrexate (MTX), has adverse short-term effects on bone. There are no studies that demonstrate the long-term response of bone to MTX. The purpose of this study was to determine the short- and long-term effects of MTX on bone volume, turnove...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/8756-3282(94)00032-u

    authors： Wheeler DL,Vander Griend RA,Wronski TJ,Miller GJ,Keith EE,Graves JE

    更新日期：1995-02-01 00:00:00

  • Increased sensitivity of rheumatoid synoviocytes to Schnurri-3 expression in TNF-α and IL-17A induced osteoblastic differentiation.

    abstract：OBJECTIVE:To compare the effects of TNF-α and IL-17A on osteogenic differentiation of isolated fibroblast-like synoviocytes (FLS) from healthy donors, osteoarthritis (OA) and rheumatoid arthritis (RA) patients. METHODS:FLS were cultured in osteogenic medium, with and without TNF-α and/or IL-17A. Extracellular matrix m...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2016.04.008

    authors： Lavocat F,Osta B,Miossec P

    更新日期：2016-06-01 00:00:00

  • Gastrodin: an ancient Chinese herbal medicine as a source for anti-osteoporosis agents via reducing reactive oxygen species.

    abstract：:Increased levels of reactive oxygen species (ROS) are a crucial pathogenic factor of osteoporosis. Gastrodin, isolated from the traditional Chinese herbal agent Gastrodia elata, is a potent antioxidant. We hypothesized that gastrodin demonstrates protective effects against osteoporosis by partially reducing reactive o...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2014.12.059

    authors： Huang Q,Shi J,Gao B,Zhang HY,Fan J,Li XJ,Fan JZ,Han YH,Zhang JK,Yang L,Luo ZJ,Liu J

    更新日期：2015-04-01 00:00:00

  • Effect of zoledronic acid on incorporation of a bioceramic bone graft substitute.

    abstract：:Many osteoporotic fracture patients are candidates for concurrent treatment with bisphosphonates and bioceramic bone graft substitutes. Osteopromotive silica-based bioactive glasses are known to induce accelerated local bone turnover and adjunct antiresorptive agents, such as zoledronic acid, may affect the process. T...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2005.09.016

    authors： Välimäki VV,Moritz N,Yrjans JJ,Vuorio E,Aro HT

    更新日期：2006-03-01 00:00:00

  • Mineralization defects in cementum and craniofacial bone from loss of bone sialoprotein.

    abstract：:Bone sialoprotein (BSP) is a multifunctional extracellular matrix protein found in mineralized tissues, including bone, cartilage, tooth root cementum (both acellular and cellular types), and dentin. In order to define the role BSP plays in the process of biomineralization of these tissues, we analyzed cementogenesis,...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2015.05.007

    authors： Foster BL,Ao M,Willoughby C,Soenjaya Y,Holm E,Lukashova L,Tran AB,Wimer HF,Zerfas PM,Nociti FH Jr,Kantovitz KR,Quan BD,Sone ED,Goldberg HA,Somerman MJ

    更新日期：2015-09-01 00:00:00

  • Characterisation of cytosolic FK506 binding protein 12 and its role in modulating expression of Cbfa1 and osterix in ROS 17/2.8 cells.

    abstract：:FK506 is a commonly used immunosuppressant that mediates its action by exclusively interacting with the cytosolic immunophilin, FK506 binding protein 12 (FKBP12). Although FK506-induced acute osteoporosis is now well recognised, its precise mode of action in osteoblasts remains unclear. Therefore, in the present study...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2004.09.021

    authors： Varanasi SS,Datta HK

    更新日期：2005-02-01 00:00:00

  • Aerobic and resistance training improve alveolar bone quality and interferes with bone-remodeling during orthodontic tooth movement in mice.

    abstract：:The direct effects of physical activity on long bones are already recognized. However, little information is available regarding distant osseous sites, such as maxillary bone. We evaluated the influence of physical training on alveolar bone quality, with and without mechanically-induced load during orthodontic tooth m...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2020.115496

    authors： Pereira LJ,Macari S,Coimbra CC,Pereira TDSF,Barrioni BR,Gomez RS,Silva TA,Paiva SM

    更新日期：2020-09-01 00:00:00

  • A new method of comprehensive static histomorphometry applied on human lumbar vertebral cancellous bone.

    abstract：:The aim of the present study was to assess age-related changes in the human spine by use of established static histomorphometry, and to determine how these static histomorphometric measures are interrelated in human cancellous bone tissue. The material comprised normal human lumbar vertebral bodies (L-2) from 12 women...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/s8756-3282(00)00285-4

    authors： Thomsen JS,Ebbesen EN,Mosekilde L

    更新日期：2000-07-01 00:00:00

  • The effects of local insulin delivery on diabetic fracture healing.

    abstract：:Several studies have documented that diabetes impairs bone healing clinically and experimentally. Systemic insulin treatment has been shown to ameliorate impaired diabetic bone healing. However, these studies failed to distinguish between a direct and a systemic effect of insulin upon bone healing. A novel intramedull...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2005.04.039

    authors： Gandhi A,Beam HA,O'Connor JP,Parsons JR,Lin SS

    更新日期：2005-10-01 00:00:00