Abstract:
INTRODUCTION:Gene-modified cell vaccines are now considered to be the best way to achieve immunotherapy for a variety of cancers including prostate cancer (PCa). XAGE-1b is a member of the cancer/testis antigen family which has demonstrated strong immunogenicity. We investigated whether XAGE-1b is an ideal target for PCa immunotherapy. MATERIALS AND METHODS:The recombinant eukaryotic expression vector pDisplay-XAGE-1b was constructed. Then the recombinant vector was transfected into Myc-CaP cells and its immunogenicity in vitro was studied. After transfection, the Myc-CaP-XAGE-1b cells were injected into FVB mice subcutaneously. Tumor growth was periodically observed and the anti-tumor effect and mechanism in vivo were further studied. RESULTS:The recombinant vector was correctly constructed by DNA sequencing and restriction endonuclease digestion. Myc-CaP cells were successfully transfected with XAGE-1b gene by immunofluorescence staining and Western blot. The transfected cells exhibited increased IFN-γ secretion, decreased IL-6 secretion and enhanced killing activity. Tumor grew slower in XAGE-1b-modified FVB mice than in wild-type FVB mice. High dendritic cell expression and low myeloid-derived suppressor cell expression were observed in tumor tissues expressed with XAGE-1b. CONCLUSIONS:XAGE-1b gene transfection could significantly enhance the immunogenicity of Myc-CaP cells. Therefore, XAGE-1b may be an attractive target for antigen-specific immunotherapy in PCa.
journal_name
Urol Intjournal_title
Urologia internationalisauthors
Xie C,Wang Gdoi
10.1159/000363333subject
Has Abstractpub_date
2015-01-01 00:00:00pages
354-62issue
3eissn
0042-1138issn
1423-0399pii
000363333journal_volume
94pub_type
杂志文章abstract::A dermoid cyst is a particular form of a cystic teratoma. In the testis, it is very rare, with only a few cases reported in world literature. A 19-year-old patient come to our attention with a 7-year history of an intratesticular cystic mass. Sonography showed a large hypoechoic solid mass. The tumour markers alpha-fe...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000094824
更新日期:2006-01-01 00:00:00
abstract::Adrenal cysts are very rare lesions, usually asymptomatic or without characteristic symptoms. They are classified as pseudocysts, endothelial cysts, epithelial cysts or parasitic cysts. Although pseudocysts are reported to be the most common clinically recognized adrenal cysts in surgical series, endothelial cysts are...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000111726
更新日期:2008-01-01 00:00:00
abstract:OBJECTIVE:This study aims to examine the relationship between chronic prostatic inflammation and prostatic calculi, and clinical parameters of benign prostatic hyperplasia (BPH). MATERIALS AND METHODS:This study was based on 225 patients who underwent transurethral resection of the prostate for BPH. Chronic inflammati...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000342643
更新日期:2013-01-01 00:00:00
abstract::Ischemia, induced by atherosclerosis, is a common cause of disorders in the elderly. Bladder dysfunction in older people may be caused by detrusor ischemia. We compared blood flow to the bladder and detrusor function in vivo and in vitro in young (6-month-old) and aged (24-month-old) male Sprague-Dawley rats. In both ...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000030365
更新日期:1999-01-01 00:00:00
abstract:BACKGROUND:Fournier's gangrene (FG) is a very aggressive necrotizing fasciitis involving subcutaneous fat and skin of scrotal and perineal regions. Vacuum-assisted closure (VAC) is a well-known method used to treat complex wounds. The authors for the first time enhance a multimodal strategy to treat the FG using VAC, r...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000218532
更新日期:2009-01-01 00:00:00
abstract:OBJECTIVE:This present study was undertaken to determine the levels of malondialdehyde (MDA), nitric oxide (NO) and total antioxidant status (TAS) in testes of adolescent rats with experimental bilateral varicocele and to determine the effects of oxidative stress on testis produced by varicocele. METHODS:6-week-old, m...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000081596
更新日期:2004-01-01 00:00:00
abstract:OBJECTIVE:Laparoscopic nephroureterectomy (LNU) offers a superior morbidity profile compared with open nephroureterectomy (ONU) in treating upper urinary tract urothelial cell carcinoma. Evidence of oncological equivalence between LNU and ONU is limited. We compare operative and oncological outcomes for LNU and ONU usi...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000364833
更新日期:2015-01-01 00:00:00
abstract::Cyclic dysmenorrhea, unilateral abdominopelvic mass, acute urinary retention and renal agenesis ipsilateral to the pelvic mass in a young woman suggest uterus didelphys with unilaterally imperforate vagina. ...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000282018
更新日期:1987-01-01 00:00:00
abstract::The measurement of sodium and water loss after relieve of the obstruction shows that the obstructive nephropathy originates in three pathogenetic mechanisms. (1) Acute complete bilateral ureteric obstruction causes tubular atrophy, prevents both resorption and glomerular filtration, reduces the renal blood flow and in...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000280270
更新日期:1979-01-01 00:00:00
abstract::Loin pain haematuria (LPHS) is a rare and difficult-to-diagnose syndrome. Different therapeutic approaches have been used historically with little or no success. We report a case of LPHS in which bilateral renal autotransplantation led to pain relief, cessation of all medication and no recurrence beyond two years of f...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000430503
更新日期:2017-01-01 00:00:00
abstract::Of 15 patients with primary aldosteronism, 7 had idiopathic adrenal hyperplasia (IHA) and 8 had aldosterone-producing adenoma (APA). In order to determine any renal problems involved in the treatment, the renal clearance of these patients was analyzed and the results compared with those obtained from 12 patients with ...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000281962
更新日期:1987-01-01 00:00:00
abstract::Splenic metastases of solid tumors are exceptional. We report the first case of an isolated splenic metastasis from prostate carcinoma, 5 years after radical prostatectomy. The splenic tumor was revealed by a pain and progressive increase in the serum prostate-specific antigen (PSA) level. Histology of the spleen show...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000096942
更新日期:2007-01-01 00:00:00
abstract::Background/Aims/Objectives: We have investigated the clinical and physiological effects of Transfer Capacitive Resistive Energy (TCARE) therapy on men with Peyronie's disease (PD). METHODS:Ninety-six men with PD have been randomized in a 2:1 ratio to receive 3 sessions of TCARE therapy or sham therapy. Pain, penile c...
journal_title:Urologia internationalis
pub_type: 杂志文章,随机对照试验
doi:10.1159/000467396
更新日期:2017-01-01 00:00:00
abstract::Experiments were performed to explore the influence of noradrenaline and isoproterenol on the ureteral peristalsis in the canine pelviureteral system in vitro. The rhythmic changes of intrapelvic pressure no longer became manifest, and simultaneously recorded electromyograms revealed disappearance of peristalsis at si...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000280908
更新日期:1983-01-01 00:00:00
abstract::Presuming that complications associated with ureteral stenting vary in type and occurrence depending on the material and cross-section of the stent, six types of stents immersed in 48 different preparations of artificial urine for 1 month to observe surface changes with a scanning electron microscope. As a result, the...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000282206
更新日期:1991-01-01 00:00:00
abstract::A 63-year-old man underwent inguinal orchiectomy for painless enlargement of the left testis. The diagnosis of malignant gonadal stromal tumor was made on the basis of morphology and immunohistochemical studies. At computed tomography there was bulky retroperitoneal metastasis. The patient then underwent retroperitone...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000283061
更新日期:1997-01-01 00:00:00
abstract:OBJECTIVE:To evaluate the clinical and demographic findings of children with urolithiasis in eastern Turkey. METHODS:We retrospectively reviewed the medical records of 67 children with urolithiasis for clinical and laboratory data. RESULTS:Mean age at the time of diagnosis was 39.1 +/- 41.2 months. Complaints at admi...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000230027
更新日期:2009-01-01 00:00:00
abstract::We report a case of Klinefelter's syndrome associated with unilateral cryptorchidism and chordee without hypospadia. The literature is reviewed, and hormonal conditions of Klinefelter's syndrome during childhood are discussed. ...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000282369
更新日期:1992-01-01 00:00:00
abstract:INTRODUCTION:Silicone has been the standard prosthesis material for the last three decades but new materials are being searched because of the known disadvantages of silicone such as migration and high cost. We wanted to test in rats whether or not polymethylmethacrylate could be an alternative to silicone. MATERIALS ...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000075277
更新日期:2004-01-01 00:00:00
abstract:AIM:The aim of this study was to compare the postoperative analgesic effectiveness of the 2 block types. We also aimed to evaluate the effect of these block types on the postoperative complications and parental satisfaction. MATERIALS AND METHODS:This prospective observational study was conducted between April and Jul...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000509173
更新日期:2020-01-01 00:00:00
abstract::A review of the recent literature on the surgical management of anterior urethral stricture was performed. The literature was searched via PubMed using the search terms 'urethral stricture' and 'urethroplasty' from 1996 to 2009. The management of anterior urethral strictures is changing rapidly in the sense that the r...
journal_title:Urologia internationalis
pub_type: 杂志文章,评审
doi:10.1159/000319501
更新日期:2011-01-01 00:00:00
abstract::The effect of vinblastine sulfate on the acceptance and growth of a very malignant, human adenocarcinoma of the kidney (RCCI) on nude mice was investigated. Without any treatment, this tumor has an acceptance rate of 100%. Subcutaneously transplanted pieces of tumor, measuring 3 X 3 X 1 mm, commence to grow rapidly 1 ...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000280404
更新日期:1981-01-01 00:00:00
abstract::Chromosomal anomalies may be a reason for male infertility. To find out whether this is true mitotic chromosome studies were performed on 41 males with azoospermia and 61 males with oligozoospermia. A constitutional chromosomal abnormality was diagnosed in 14 (34.1%) azoospermic patients and in 2 (3.3%) oligozoospermi...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000030280
更新日期:1998-10-01 00:00:00
abstract::24 h urinary pyrophosphate excretion was studied in 20 normal healthy subjects and 75 idiopathic stone formers from north-western regions of India. The mean 24-hour urinary excretion of pyrophosphate was significantly low in stone formers (50.67 +/- 2.16 mumol/24 h) as compared to that of normal subjects (71.46 +/- 5....
journal_title:Urologia internationalis
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1159/000282364
更新日期:1992-01-01 00:00:00
abstract::Regenerative medicine, tissue engineering and biomedical research give hope to many patients who need bio-implants. Tissue engineering applications have already been developed based on bioreactors. Physiological ureter implants, however, do not still function sufficiently, as they represent tubular hollow structures w...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000368419
更新日期:2015-01-01 00:00:00
abstract::The sequential expressed prostatic secretion (EPS) levels of latamoxef (LMOX) were measured in 14 patients with acute bacterial prostatitis to evaluate the diffusion of this antibiotic from the plasma into the prostatic fluid. All patients received 2 g of LMOX on the 1st, 4th, and 7th days of hospitalization. The mean...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000282760
更新日期:1995-01-01 00:00:00
abstract:OBJECTIVE:The aim of this study is to compare vesical and renal calcium oxalate crystalluria in an attempt to correlate crystal formation with chemical composition and calcium oxalate saturation of renal urine. MATERIAL AND METHODS:Urine specimens were directly collected from the bladder and the kidney, of 11 stone fo...
journal_title:Urologia internationalis
pub_type: 临床试验,杂志文章
doi:10.1159/000030201
更新日期:1998-01-01 00:00:00
abstract::High concentrations of phosphate, oxalate, and calcium ions in urine may cause formation of mineral deposits, i.e., urolithiasis. This is prevented in healthy individuals by substances present in trace quantities. However, there is no recognizable difference between normals and stone formers in urinary substance conte...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000281098
更新日期:1985-01-01 00:00:00
abstract:INTRODUCTION:We investigated the relationship between posterior vault prolapse and overactive bladder (OAB) symptoms or detrusor overactivity (DO) and their changes after surgical repair. METHODS:Forty-three patients with vault prolapse and prevalent posterior compartment prolapse underwent pelvic organ prolapse repai...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000345944
更新日期:2013-01-01 00:00:00
abstract::Twenty males underwent transrectal ultrasonography before and after ejaculation to examine possible alterations that could influence interpretation of seminal vesicle ultrasonography. The preejaculation length of 35 mm was significantly (p < 0.05) decreased to 30 mm after ejaculation. The mean width of the seminal ves...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000282404
更新日期:1992-01-01 00:00:00