Pilot study of a novel vacuum-assisted method for decellularization of tracheae for clinical tissue engineering applications.

abstract：:Cartilage defects heal imperfectly and osteoarthritic changes develop frequently as a result. Although the existence of specific behaviours of chondrocytes derived from various depth-related zones in vitro has been known for over 20 years, only a relatively small body of in vitro studies has been performed with zonal ...

journal_title：Journal of tissue engineering and regenerative medicine

authors： Schuurman W,Klein TJ,Dhert WJ,van Weeren PR,Hutmacher DW,Malda J

更新日期：2015-06-01 00:00:00

abstract：:Mesenchymal stem cells (MSCs) have been suggested as a potential cell source for tendon/ligament tissue engineering. Extrinsic cues, such as the chemical and physical properties of scaffolds, as well as external forces, play an important role in fibroblastic differentiation of these cells. In this study, we employed a...

journal_title：Journal of tissue engineering and regenerative medicine

authors： Qiu Y,Lei J,Koob TJ,Temenoff JS

更新日期：2016-12-01 00:00:00

abstract：:The aim of this study was to investigate the effects of a novel bioactive material (Biosilicate®) and low-level laser therapy (LLLT) on bone fracture consolidation in osteoporotic rats. Forty female Wistar rats were submitted to ovariectomy (OVX) to induce osteopenia. Eight weeks after surgery, the animals were random...

journal_title：Journal of tissue engineering and regenerative medicine

authors： Bossini PS,Rennó AC,Ribeiro DA,Fangel R,Peitl O,Zanotto ED,Parizotto NA

更新日期：2011-03-01 00:00:00

abstract：:Spinal cord injury (SCI) is a neurological disorder that arises from a primary acute mechanical lesion, followed by a pathophysiological cascade of events that leads to further spinal cord tissue damage. Several preclinical and clinical studies have highlighted the ability of stem cell therapy to improve long-term fun...

journal_title：Journal of tissue engineering and regenerative medicine

authors： Mammana S,Gugliandolo A,Cavalli E,Diomede F,Iori R,Zappacosta R,Bramanti P,Conti P,Fontana A,Pizzicannella J,Mazzon E

更新日期：2019-07-01 00:00:00

abstract：:Peripheral human nerves fail to regenerate across long tube implants (>2 cm), and tissue-engineered nerve grafts represent a promising treatment alternative. The present study aims to investigate the testosterone propionate (TP) repair effect of acellular nerve allograft (ANA) seeded with allogeneic bone marrow mesenc...

journal_title：Journal of tissue engineering and regenerative medicine

authors： Chen C,Tian Y,Wang J,Zhang X,Nan L,Dai P,Gao Y,Zheng S,Liu W,Zhang Y

更新日期：2019-09-01 00:00:00

abstract：:Spatiotemporal changes in the extracellular matrix (ECM) were studied within abdominal aortic aneurysms (AAAs) generated in rats via elastase infusion. At 7, 14 and 21 days post-induction, AAA tissues were divided into proximal, mid- and distal regions, based on their location relative to the renal arteries and the re...

journal_title：Journal of tissue engineering and regenerative medicine

authors： Deb PP,Ramamurthi A

更新日期：2017-01-01 00:00:00

abstract：:The advances achieved by cell-based therapies to treat autoimmune diseases such as rheumatoid arthritis (RA) and multiple sclerosis (MS), despite promising, are still insufficient for the current demands. RA and MS therapeutic approaches follow world guidelines to use disease modifying drugs and biological agents that...

journal_title：Journal of tissue engineering and regenerative medicine

authors： Rodrigues DB,Oliveira JM,Santos TC,Reis RL

更新日期：2018-03-01 00:00:00

abstract：:Rapid vascular remodelling of damaged dermal tissue is required to heal burn wounds. Thymosin β4 (Tβ4) is a growth factor that has been shown to promote angiogenesis and dermal wound repair. However, the underlying mechanisms based on Tβ4 function have not yet been fully investigated. In the present study, we investig...

journal_title：Journal of tissue engineering and regenerative medicine

authors： Kim S,Kwon J

更新日期：2017-04-01 00:00:00

abstract：:The combination of scaffolds and mesenchymal stromal cells (MSCs) is a promising approach in bone tissue engineering (BTE). Knowledge on the survival, outgrowth and bone-forming capacity of MSCs in vivo is limited. Bioluminescence imaging (BLI), histomorphometry and immunohistochemistry were combined to study the fate...

journal_title：Journal of tissue engineering and regenerative medicine

authors： Prins HJ,Fernandes H,Rozemuller H,van Blitterswijk C,de Boer J,Martens AC

更新日期：2016-03-01 00:00:00

abstract：:Electrospinning has recently gained widespread attention as a process capable of producing nanoscale fibres that mimic native extracellular matrix. In this study, we compared the osteogenic differentiation behaviour of human adipose stem cells (ASCs) on a 3D nanofibre matrix of type I rat tail collagen (RTC) and a 2D ...

journal_title：Journal of tissue engineering and regenerative medicine

authors： Sefcik LS,Neal RA,Kaszuba SN,Parker AM,Katz AJ,Ogle RC,Botchwey EA

更新日期：2008-06-01 00:00:00

abstract：:Recellularization of ex vivo-derived scaffolds remains a significant hurdle primarily due to the scaffolds subcellular pore size that restricts initial cell seeding to the scaffolds periphery and inhibits migration over time. With the aim to improve cell migration, repopulation, and graft mechanics, the effects of a f...

journal_title：Journal of tissue engineering and regenerative medicine

authors： Van de Walle AB,Moore MC,McFetridge PS

更新日期：2020-03-01 00:00:00

abstract：:The present study aimed to investigate the effect of structure (design and porosity) on the matrix stiffness and osteogenic activity of stem cells cultured on poly(ester-urethane) (PEU) scaffolds. Different three-dimensional (3D) forms of scaffold were prepared from lysine-based PEU using traditional salt-leaching and...

journal_title：Journal of tissue engineering and regenerative medicine

authors： Kiziltay A,Marcos-Fernandez A,San Roman J,Sousa RA,Reis RL,Hasirci V,Hasirci N

更新日期：2015-08-01 00:00:00

abstract：:This work reports the effects of phosphoserine addition on the biodegradability of calcium phosphate cements. The characteristics of a phosphoserine-modified calcium phosphate cement without collagen in a large animal model are presented here for the first time. Critical size bone defects in the proximal tibia of 10 s...

journal_title：Journal of tissue engineering and regenerative medicine

authors： Offer L,Veigel B,Pavlidis T,Heiss C,Gelinsky M,Reinstorf A,Wenisch S,Lips KS,Schnettler R

更新日期：2011-01-01 00:00:00

abstract：:Our recent clinical studies have demonstrated that autologous implantation of human cultured periosteal (hCP) sheets in combination with porous hydroxylapatite (HAp) particles at the site of periodontal bone defects strikingly facilitates tissue regeneration. To better understand how the hCP sheet functions at the imp...

journal_title：Journal of tissue engineering and regenerative medicine

authors： Kawase T,Okuda K,Kogami H,Nakayama H,Nagata M,Nakata K,Yoshie H

更新日期：2009-03-01 00:00:00

abstract：:Tissue engineering of skeletal muscle is an encouraging possibility for the treatment of muscle loss through the creation of functional muscle tissue in vitro from human stem cells. Currently, the preferred stem cells are primary, non-immunogenic satellite cells ( = myoblasts). The objective of this study was to deter...

journal_title：Journal of tissue engineering and regenerative medicine

authors： Stern-Straeter J,Bonaterra GA,Kassner SS,Zügel S,Hörmann K,Kinscherf R,Goessler UR

更新日期：2011-08-01 00:00:00

abstract：:Transplantation of mesenchymal stem cells (MSCs) has been proposed to exert beneficial effects on peripheral nerve regeneration after a peripheral nerve injury, but the functional recovery in the denervated limb is still limited. In this study, we used low-level laser therapy (LLLT) as an adjunct therapy for MSC trans...

journal_title：Journal of tissue engineering and regenerative medicine

authors： Yang CC,Wang J,Chen SC,Hsieh YL

更新日期：2016-02-01 00:00:00

abstract：BACKGROUND:According to experimental studies, mesh implants may reduce in size up to 50.8% during their integration into soft tissues. This results in impaired mobility of the anterior abdominal wall and hernia recurrences, as well as affects patients' quality of life. Due to unsatisfactory radiographic contrast of pol...

journal_title：Journal of tissue engineering and regenerative medicine

authors： Shemyatovsky KA,Azimov RH,Alekhin AI,Kazantsev AA,Alekhin AA

更新日期：2020-05-01 00:00:00

abstract：:Biomaterials capable of controlling the release of multiple growth factors (GFs) could potentially promote the integration of co-transplanted neural progenitor cells (NPCs) and stimulate the plasticity and regenerability of the lesioned spinal cord. As a first step towards the employment of such a vehicle for cell the...

journal_title：Journal of tissue engineering and regenerative medicine

authors： Cizkova D,Slovinska L,Grulova I,Salzet M,Cikos S,Kryukov O,Cohen S

更新日期：2015-08-01 00:00:00

abstract：:Nonvascularized fat grafting is a valuable technique for soft tissue reconstruction but poor survival of fat in the host environment remains a problem. A process known as cell-assisted transfer is used to enhance fat graft retention by adding stromal vascular fraction, an adipose-derived stem cell (ASC) rich content t...

journal_title：Journal of tissue engineering and regenerative medicine

authors： Tan SS,Zhan W,Poon CJ,Han X,Marre D,Boodhun S,Palmer JA,Mitchell GM,Morrison WA

更新日期：2018-02-01 00:00:00

abstract：:Poly(glycerol sebacate) (PGS) is a biodegradable elastomer that has generated great interest as a scaffold material due to its desirable mechanical properties. However, the use of PGS in tissue engineering is limited by difficulties in casting micro- and nanofibrous structures, due to high temperatures and vacuum requ...

journal_title：Journal of tissue engineering and regenerative medicine

authors： Sant S,Hwang CM,Lee SH,Khademhosseini A

更新日期：2011-04-01 00:00:00

abstract：:This study reports the manufacturing process of 3D interconnected macroporous tricalcium phosphate (TCP) scaffolds with controlled internal architecture by direct 3D printing (3DP), and high mechanical strength obtained by microwave sintering. TCP scaffolds with 27%, 35% and 41% designed macroporosity with pore sizes ...

journal_title：Journal of tissue engineering and regenerative medicine

authors： Tarafder S,Balla VK,Davies NM,Bandyopadhyay A,Bose S

更新日期：2013-08-01 00:00:00

abstract：:Tendon healing still represents a challenge for clinicians because it is slow and incomplete. The most injured is the Achilles tendon, and surgery is the therapeutic strategy often adopted because it provides a quicker functional recovery. Peritendinous adhesions are the main complication of surgery with hyperplasia a...

journal_title：Journal of tissue engineering and regenerative medicine

authors： Veronesi F,Giavaresi G,Bellini D,Casagranda V,Pressato D,Fini M

更新日期：2020-08-01 00:00:00

abstract：:Nanopatterned (330 nm wide channels) type I collagen films were prepared by solvent casting on poly(dimethyl siloxane) (PDMS) templates. These films were rolled into tubular constructs and crosslinked. Tubular constructs were incubated under cell culture conditions for 28 days and examined by stereomicroscopy and scan...

journal_title：Journal of tissue engineering and regenerative medicine

authors： Zorlutuna P,Hasirci N,Hasirci V

更新日期：2008-08-01 00:00:00

abstract：:Tissue engineering applications require an appropriate combination of a cell population, biochemical factors and scaffold materials. In this field, mesenchymal stem cells (MSCs) emerge as an attractive cell population, due to their ready availability and their potential to be differentiated into various mesodermal cel...

journal_title：Journal of tissue engineering and regenerative medicine

authors： Sart S,Errachid A,Schneider YJ,Agathos SN

更新日期：2013-07-01 00:00:00

abstract：:Enhancement of osteogenic capacity was achieved in a mineralized collagen composite, nano-hydroxyapatite/collagen (nHAC), by loading with synthetic peptides derived from BMP-2 residues 32-48 (P17-BMP-2). Rabbit marrow stromal cells (MSCs) were used in vitro to study cell biocompatibility, attachment and differentiatio...

journal_title：Journal of tissue engineering and regenerative medicine

authors： Zhang X,Guo WG,Cui H,Liu HY,Zhang Y,Müller WE,Cui FZ

更新日期：2016-02-01 00:00:00

abstract：:Bone marrow (BM) multipotent mesenchymal stromal cells (MSCs) present with multipotent differentiation potential and immunomodulatory properties. As an alternative to bone marrow, we have examined fetal membranes, amnion and chorion, of term human placenta as a potential source of multipotent MSCs. Here we show that a...

journal_title：Journal of tissue engineering and regenerative medicine

authors： Soncini M,Vertua E,Gibelli L,Zorzi F,Denegri M,Albertini A,Wengler GS,Parolini O

更新日期：2007-07-01 00:00:00

abstract：:Keratin is an important protein used for wound healing and tissue recovery. In this study, keratin was first extracted from raw materials and chemically modified to obtain stable keratin (m-keratin). The raw and m-keratin were examined by Raman spectroscopy. The molecular weight of the m-keratin was analysed by SDS-PA...

journal_title：Journal of tissue engineering and regenerative medicine

authors： Yuan J,Geng J,Xing Z,Shim KJ,Han I,Kim JC,Kang IK,Shen J

更新日期：2015-09-01 00:00:00

abstract：:Repairing the peripheral nerves following a segmental defect injury remains surgically challenging. Because of some disadvantages of nerve grafts, nerve regeneration, such as conduits combined with bone marrow-derived mesenchymal stem cells (BMSCs), may serve as an alternative. BMSCs expand under hypoxic conditions, d...

journal_title：Journal of tissue engineering and regenerative medicine

authors： Wang JP,Liao YT,Wu SH,Chiang ER,Hsu SH,Tseng TC,Hung SC

更新日期：2020-12-01 00:00:00

abstract：:In this study, a new foaming method, based on physical foaming combined with microwave-induced curing, is proposed in combination with a surface bioactivation to develop scaffold for bone tissue regeneration. In the first step of the process, a stable physical foaming was induced using a surfactant (Pluronic) as blowi...

journal_title：Journal of tissue engineering and regenerative medicine

authors： Demitri C,Giuri A,De Benedictis VM,Raucci MG,Giugliano D,Sannino A,Ambrosio L

更新日期：2017-01-01 00:00:00

abstract：:Human mesenchymal stem cells (hMSCs) are typically obtained for research or therapeutic applications by isolating and subculturing adherent cells from bone marrow on tissue-culture substrates using growth media. The purity and properties of the resulting populations can be affected greatly by the conditions under whic...

journal_title：Journal of tissue engineering and regenerative medicine

authors： Jung S,Sen A,Rosenberg L,Behie LA

更新日期：2012-05-01 00:00:00